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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS04835 [old locus tag: SAUSA300_0897 ]
- pan locus tag?: SAUPAN003163000
- symbol: SAUSA300_RS04835
- pan gene symbol?: trpS
- synonym:
- product: tryptophan--tRNA ligase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS04835 [old locus tag: SAUSA300_0897 ]
- symbol: SAUSA300_RS04835
- product: tryptophan--tRNA ligase
- replicon: chromosome
- strand: -
- coordinates: 985686..986675
- length: 990
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (985686..986675) NCBI
- BioCyc: SAUSA300_RS04835 BioCyc
- MicrobesOnline: see SAUSA300_0897
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGGAGACATTATTTTCAGGCATCCAACCTAGTGGAATTCCTACTATTGGAAATTATATT
GGCGCACTAAAACAATTTGTTGATGTGCAAAATGACTATGATTGTTATTTCTGTATCGTA
GATCAACATGCAATTACAATGCCACAAGATCGTTTAAAATTACGTAAACAGACCAGACAA
TTAGCAGCGATTTATTTAGCTTCTGGTATAGATCCAGACAAAGCAACATTGTTCATACAA
TCTGAAGTCCCTGCACACGTACAAGCAGGATGGATGTTAACTACGATTGCTTCTGTTGGA
GAATTAGAGCGTATGACGCAATACAAAGATAAAGCTCAGAAAGCAGTTGAAGGTATACCT
GCTGGTCTATTAACATATCCACCTTTAATGGCAGCTGATATTGTTCTTTACAATACTAAT
ATCGTTCCAGTTGGAGATGACCAAAAGCAGCATATCGAATTGACTCGTAACCTTGTAGAT
AGATTTAATAGTCGCTATAATGATGTGCTTGTGAAACCTGAAATTCGTATGCCTAAAGTT
GGTGGACGTGTCATGAGTTTACAAGACCCAACAAGAAAAATGAGTAAGAGTGATGATAAT
GCTAAAAACTTCATTTCATTATTAGACGAGCCGAATGTTGCAGCTAAAAAAATTAAAAGC
GCAGTAACTGATTCAGATGGTATTATTAAATTTGATCGTGACAACAAGCCAGGTATAACA
AATTTAATTTCAATATACGCTGGATTAACAGACATGCCAATTAAAGATATTGAGGCAAAA
TATGAGGGCGAAGGTTATGGTAAATTTAAAGGTGACCTTGCTGAAATAGTTAAAGCATTT
TTAGTAGAATTCCAAGAAAAATACGAAAGTTTCTATAACTCAGATAAACTTGATGATATT
TTAGATCAAGGTAGAGATAAAGCACACAAAGTTTCATTTAAAACTGTCAAAAAAATGGAA
AAAGCGATGGGTTTAGGACGTAAGAGATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS04835 [old locus tag: SAUSA300_0897 ]
- symbol: SAUSA300_RS04835
- description: tryptophan--tRNA ligase
- length: 329
- theoretical pI: 8.14756
- theoretical MW: 36909.2
- GRAVY: -0.387538
⊟Function[edit | edit source]
- reaction: EC 6.1.1.2? ExPASyTryptophan--tRNA ligase ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)
- TIGRFAM: Protein synthesis tRNA aminoacylation tryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 362.5)
- TheSEED: see SAUSA300_0897
- PFAM: HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 266.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 10
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007356
- TAT(Tat/SPI): 0.000216
- LIPO(Sec/SPII): 0.001212
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 446371079 NCBI
- RefSeq: WP_000448934 NCBI
- UniProt: see SAUSA300_0897
⊟Protein sequence[edit | edit source]
- METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHAITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGELERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVDRFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNAKNFISLLDEPNVAAKKIKSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEIVKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS03530 LysR family transcriptional regulator [1] (data from MRSA252) SAUSA300_RS04095 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [1] (data from MRSA252) SAUSA300_RS04885 NAD(+) kinase [1] (data from MRSA252) SAUSA300_RS05290 phosphocarrier protein HPr [1] (data from MRSA252) SAUSA300_RS07035 cold-shock protein CspA [1] (data from MRSA252) SAUSA300_RS08135 elongation factor P [1] (data from MRSA252) SAUSA300_RS11315 UDP-N-acetylglucosamine 1-carboxyvinyltransferase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)