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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus USA300_FPR3757
- locus tag: SAUSA300_RS11440 [old locus tag: SAUSA300_2078 ]
- pan locus tag?: SAUPAN005416000
- symbol: SAUSA300_RS11440
- pan gene symbol?: murA2
- synonym:
- product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAUSA300_RS11440 [old locus tag: SAUSA300_2078 ]
- symbol: SAUSA300_RS11440
- product: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
- replicon: chromosome
- strand: -
- coordinates: 2239704..2240963
- length: 1260
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Location: NC_007793 (2239704..2240963) NCBI
- BioCyc: SAUSA300_RS11440 BioCyc
- MicrobesOnline: see SAUSA300_2078
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1201ATGGCTCAAGAGGTAATAAAAATAAGAGGTGGACGCACACTAAATGGGGAAGTTAATATC
AGTGGCGCGAAAAACAGTGCTGTAGCTATCATTCCTGCAACATTATTAGCTCAAGGACAT
GTGAAATTAGAAGGGTTACCGCAAATCTCTGATGTTAAAACTTTAGTGAGTTTATTAGAA
GATTTAAATATTAAGGCATCACTAAATGGTACGGAATTAGAAGTTGATACAACTGAAATA
CAAAATGCTGCATTACCAAATAATAAAGTTGAATCATTACGTGCTTCATACTATATGATG
GGTGCCATGTTAGGTAGATTTAAAAAATGTGTGATTGGTTTACCGGGAGGATGTCCACTT
GGGCCACGTCCAATTGATCAACATATTAAAGGATTTAAAGCTTTAGGTGCTGAAATTGAT
GAATCAAGTACGACATCAATGAAAATCGAAGCTAAAGAGTTAAAAGGTGCACATATTTTT
CTAGATATGGTTAGCGTAGGTGCAACAATTAACATTATGCTAGCTGCAGTTTATGCAACA
GGACAAACTGTAATTGAAAATGCTGCTAAAGAACCGGAAGTAGTTGATGTTGCTAACTTC
TTAACAAGTATGGGAGCTAATATTAAGGGAGCGGGTACATCAACAATTAAAATCAATGGC
GTCAAAGAATTACATGGTTCTGAGTATCAAGTTATACCTGATAGAATTGAAGCAGGCACA
TATATGTGTATCGCTGCAGCATGTGGAGAAAATGTTATATTAAATAATATTGTTCCGAAA
CATGTAGAAACATTAACTGCTAAATTTAGTGAATTAGGTGTGAATGTTGATGTAAGAGAC
GAAAGAATTCGCATCAATAATAATGCGCCATATCAATTTGTTGATATTAAAACACTAGTA
TATCCAGGTTTTGCTACTGATTTGCAACAGCCTATTACACCATTATTATTTATGGCAAAT
GGTCCTTCATTTGTAACTGATACGATTTATCCGGAGCGTTTTAAACATGTTGAAGAATTA
AAGCGCATGGGTGCAAATATTGAAGTTGACGAAGGCACAGCAACGATAAAACCATCAACA
TTACATGGTGCTGAAGTTTATGCTAGCGATTTAAGAGCAGGAGCTTGTTTAATTATTGCT
GGTTTAATTGCTGAGGGTGTAACTACAATTTACAATGTTAAACATATTTATAGAGGTTAT
ACAGATATTGTAGAACACTTAAAAGCTTTAGGTGCAGATATTTGGACGGAAACTGTATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAUSA300_RS11440 [old locus tag: SAUSA300_2078 ]
- symbol: SAUSA300_RS11440
- description: UDP-N-acetylglucosamine 1-carboxyvinyltransferase
- length: 419
- theoretical pI: 5.46141
- theoretical MW: 45074.7
- GRAVY: 0.072315
⊟Function[edit | edit source]
- reaction: EC 2.5.1.7? ExPASyUDP-N-acetylglucosamine 1-carboxyvinyltransferase Phosphoenolpyruvate + UDP-N-acetyl-alpha-D-glucosamine = phosphate + UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine
- TIGRFAM: Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan UDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 526.9)and 1 moreAmino acid biosynthesis Aromatic amino acid family 3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 87.8)
- TheSEED: see SAUSA300_2078
- PFAM: EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 300.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.018228
- TAT(Tat/SPI): 0.001295
- LIPO(Sec/SPII): 0.001594
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
- GI: 445968747 NCBI
- RefSeq: WP_000046602 NCBI
- UniProt: see SAUSA300_2078
⊟Protein sequence[edit | edit source]
- MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLEGLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGAMLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIFLDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKINGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNVDVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERFKHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYNVKHIYRGYTDIVEHLKALGADIWTETV
⊟Experimental data[edit | edit source]
- experimentally validated: data available for COL, NCTC8325
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
SAUSA300_RS05855 cell division protein FtsZ [1] (data from MRSA252) SAUSA300_RS06135 50S ribosomal protein L19 [1] (data from MRSA252) SAUSA300_RS06220 30S ribosomal protein S2 [1] (data from MRSA252) SAUSA300_RS11805 Asp23/Gls24 family envelope stress response protein [1] (data from MRSA252) SAUSA300_RS12120 30S ribosomal protein S3 [1] (data from MRSA252) SAUSA300_RS12125 50S ribosomal protein L22 [1] (data from MRSA252) SAUSA300_RS12150 50S ribosomal protein L3 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)