PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2070 [new locus tag: SAUSA300_RS11400 ]
• pan locus tag^?: SAUPAN005407000
• symbol: SAUSA300_2070
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2070 [new locus tag: SAUSA300_RS11400 ]
• symbol: SAUSA300_2070
• product: hypothetical protein
• replicon: chromosome
• strand: -
• coordinates: 2231704..2232750
• length: 1047
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914286 NCBI
• RefSeq: YP_494716 NCBI
• BioCyc: see SAUSA300_RS11400
• MicrobesOnline: 1293585 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  1021
  
  TTGGATACTAAAATTTGGGATGTTAGAGAATATAATGAAGATTTACAGCAATATCCTAAA
  ATTAATGAAATAAAAGACATTGTTTTAAACGGTGGTTTAATAGGTTTACCAACTGAAACA
  GTTTATGGACTTGCAGCAAATGCGACAGATGAAGAAGCTGTAGCTAAAATATATGAAGCT
  AAAGGCCGTCCATCTGACAATCCGCTTATTGTTCATATACACAGTAAAGGTCAATTAAAA
  GATTTTACATATACTTTGGATCCACGCGTAGAAAAGTTAATGCAGGCATTCTGGCCGGGC
  CCTATTTCGTTTATATTGCCGTTAAAGCTAGGCTATCTATGTCGAAAAGTTTCTGGAGGT
  TTATCATCAGTTGCTGTTAGAATGCCAAGCCATTCTGTAGGTAGACAATTATTACAAATC
  ATAAATGAACCTCTAGCTGCTCCAAGTGCTAATTTAAGTGGTAGACCTTCACCAACAACT
  TTCAATCATGTATATCAAGATTTGAATGGCCGTATCGATGGTATTGTTCAAGCTGAACAA
  AGTGAAGAAGGATTAGAAAGTACGGTTTTAGATTGCACATCTTTTCCTTATAAAATTGCA
  AGACCTGGTTCTATAACAGCAGCAATGATTACAGAAATACTTCCGAATAGTATCGCCCAT
  GCTGATTATAATGATACTGAACAGCCAATTGCACCAGGTATGAAGTATAAGCATTACTCA
  CCCAATACACCACTTACAATTATTACAGATATTGAGAGCAAAATTGGAAATGACGGTAAA
  GATTGGTCTTCTATAGCTTTTATTGTGCCGAGTAATAAGGTGGCGTTTATACCAAGTGAA
  GCGCAATTCATTCAATTATGTCAGGATGACAATGATGTTAAACAAGCAAGTCATAATCTT
  TATGATGTGTTACATTCACTTGATGAAAATGAAAATATTTCAGCGGCGTATATATACGGC
  TTTGAGCTGAATGATAATACAGAAGCAATTATGAATCGCATGTTAAAAGCTGCAGGTAAT
  CACATTATTAAAGGATGTGAACTATGA
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  1047
  

⊟Protein

⊟General

• locus tag: SAUSA300_2070 [new locus tag: SAUSA300_RS11400 ]
• symbol: SAUSA300_2070
• description: hypothetical protein
• length: 348
• theoretical pI: 4.80011
• theoretical MW: 38445.3
• GRAVY: -0.269253

⊟Function

• reaction:
  EC 2.7.7.87?  ExPASy
  L-threonylcarbamoyladenylate synthase L-threonine + ATP + HCO₃(-) = L-threonylcarbamoyladenylate + diphosphate + H₂O
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family (TIGR00057; HMM-score: 194.5)
  and 1 more
  Genetic information processing Protein fate Protein modification and repair carbamoyltransferase HypF (TIGR00143; HMM-score: 27.4)
• TheSEED  :
  • TsaC protein (YrdC-Sua5 domains) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  RNA Metabolism RNA processing and modification YrdC-YciO  TsaC protein (YrdC-Sua5 domains) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
  and 1 more
  RNA Metabolism RNA processing and modification YrdC-YciO-Sua5 and associated protein families  TsaC protein (YrdC-Sua5 domains) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
• PFAM:
  no clan defined Sua5_yciO_yrdC; Telomere recombination (PF01300; HMM-score: 179.4)
  and 1 more
  GT-B (CL0113) SUA5; Putative GTP-binding controlling metal-binding (PF03481; HMM-score: 76.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.041162
  • TAT(Tat/SPI): 0.011825
  • LIPO(Sec/SPII): 0.000612
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87162029 NCBI
• RefSeq: YP_494716 NCBI
• UniProt: A0A0H2XJW1 UniProt

⊟Protein sequence

• MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGLAANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPISFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTTFNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSIAHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAFIPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRMLKAAGNHIIKGCEL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_2069 < SAUSA300_2070 < SAUSA300_2071 < prfA < tdk

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications