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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_02357
- pan locus tag?: SAUPAN005407000
- symbol: SAOUHSC_02357
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
- Gene ID: 3919400 NCBI
- RefSeq: YP_500833 NCBI
- BioCyc: G1I0R-2226 BioCyc
- MicrobesOnline: 1290794 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1081GTGCATAGCTTTTTGCTTTATATTATGATAAATAAGAAAGGCGTGATTAAGTTGGATACT
AAAATTTGGGATGTTAGAGAATATAATGAAGATTTACAGCAATATCCTAAAATTAATGAA
ATAAAAGACATTGTTTTAAACGGTGGTTTAATAGGTTTACCAACTGAAACAGTTTATGGA
CTTGCAGCAAATGCGACAGATGAAGAAGCTGTAGCTAAAATATATGAAGCTAAAGGCCGT
CCATCTGACAATCCGCTTATTGTTCATATACACAGTAAAGGTCAATTAAAAGATTTTACA
TATACTTTGGATCCACGCGTAGAAAAGTTAATGCAGGCATTCTGGCCGGGCCCTATTTCG
TTTATATTGCCGTTAAAGCTAGGCTATCTATGTCGAAAAGTTTCTGGAGGTTTATCATCA
GTTGCTGTTAGAATGCCAAGCCATTCTGTAGGTAGACAATTATTACAAATCATAAATGAA
CCTCTAGCTGCTCCAAGTGCTAATTTAAGTGGTAGACCTTCACCAACAACTTTCAATCAT
GTATATCAAGATTTGAATGGCCGTATCGATGGTATTGTTCAAGCTGAACAAAGTGAAGAA
GGATTAGAAAGTACGGTTTTAGATTGCACATCTTTTCCTTATAAAATTGCAAGACCTGGT
TCTATAACAGCAGCAATGATTACAGAAATACTTCCGAATAGTATCGCCCATGCTGATTAT
AATGATACTGAACAGCCAATTGCACCAGGTATGAAGTATAAGCATTACTCACCCAATACA
CCACTTACAATTATTACAGATATTGAGAGCAAAATTGGAAATGACGGTAAAGATTGGTCT
TCTATAGCTTTTATTGTGCCGAGTAATAAGGTGGCGTTTATACCAAGTGAAGCGCAATTC
ATTCAATTATGTCAGGATGACAATGATGTTAAACAAGCAAGTCATAATCTTTATGATGTG
TTACATTCACTTGATGAAAATGAAAATATTTCAGCGGCGTATATATACGGCTTTGAGCTG
AATGATAATACAGAAGCAATTATGAATCGCATGTTAAAAGCTGCAGGTAATCACATTATT
AAAGGATGTGAACTATGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_02357
- symbol: SAOUHSC_02357
- description: hypothetical protein
- length: 365
- theoretical pI: 5.23175
- theoretical MW: 40444.8
- GRAVY: -0.22137
⊟Function[edit | edit source]
- reaction: EC 2.7.7.87? ExPASyL-threonylcarbamoyladenylate synthase L-threonine + ATP + HCO3(-) = L-threonylcarbamoyladenylate + diphosphate + H2O
- TIGRFAM: Protein synthesis tRNA and rRNA base modification tRNA threonylcarbamoyl adenosine modification protein, Sua5/YciO/YrdC/YwlC family (TIGR00057; HMM-score: 194.3)and 1 moreProtein fate Protein modification and repair carbamoyltransferase HypF (TIGR00143; HMM-score: 27.2)
- TheSEED :
- TsaC protein (YrdC-Sua5 domains) required for threonylcarbamoyladenosine t(6)A37 modification in tRNA
RNA Metabolism RNA processing and modification YrdC-YciO TsaC protein (YrdC-Sua5 domains) required for threonylcarbamoyladenosine t(6)A37 modification in tRNAand 1 more - PFAM: no clan defined Sua5_yciO_yrdC; Telomere recombination (PF01300; HMM-score: 179.2)and 1 moreGT-B (CL0113) SUA5; Putative GTP-binding controlling metal-binding (PF03481; HMM-score: 76.3)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.004616
- TAT(Tat/SPI): 0.000177
- LIPO(Sec/SPII): 0.000577
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MHSFLLYIMINKKGVIKLDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGLAANATDEEAVAKIYEAKGRPSDNPLIVHIHSKGQLKDFTYTLDPRVEKLMQAFWPGPISFILPLKLGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTTFNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITEILPNSIAHADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAFIPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRMLKAAGNHIIKGCEL
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [2] [3]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_02356 < SAOUHSC_02357 < SAOUHSC_02358 < prfA < SAOUHSC_02360predicted SigA promoter [4] : atpC < SAOUHSC_02341 < SAOUHSC_02343 < SAOUHSC_02345 < SAOUHSC_02346 < SAOUHSC_02347 < S904 < SAOUHSC_02349 < SAOUHSC_02350 < SAOUHSC_02351 < SAOUHSC_02352 < upp < glyA < SAOUHSC_02355 < S905 < SAOUHSC_02356 < SAOUHSC_02357 < SAOUHSC_02358 < S906 < prfA < SAOUHSC_02360
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [4]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 4.0 4.1 4.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)