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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2073 [new locus tag: SAUSA300_RS11415 ]
  • pan locus tag?: SAUPAN005410000
  • symbol: tdk
  • pan gene symbol?: tdk
  • synonym:
  • product: thymidine kinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2073 [new locus tag: SAUSA300_RS11415 ]
  • symbol: tdk
  • product: thymidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2234734..2235333
  • length: 600
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGTATGAAACTTACCATTCCGGTTGGATAGAATGTATTACAGGTAGTATGTTTAGCGGT
    AAATCCGAAGAACTAATAAGACGTCTAAGAAGGGGTATATACGCTAAACAAAAAGTCGTT
    GTATTTAAACCGGCGATTGATGATCGATATCATAAAGAAAAAGTGGTCTCTCATAATGGT
    AATGCCATTGAAGCGATTAATATTTCTAAAGCAAGTGAAATTATGACGCACGACTTAACT
    AATGTTGATGTTATAGGAATAGACGAAGTGCAATTTTTTGACGATGAAATTGTAAGTATA
    GTAGAAAAACTATCAGCAGATGGACACCGTGTTATTGTTGCTGGATTAGATATGGATTTT
    AGGGGCGAACCGTTCGAACCAATGCCTAAATTGATGGCTGTCAGTGAACAAGTAACGAAA
    CTTCAAGCAGTTTGTGCTGTTTGTGGTTCTTCATCTAGTAGAACGCAACGATTAATAAAT
    GGTAAACCAGCTAAAATCGATGATCCGATTATCTTAGTAGGTGCCAATGAAAGTTATGAG
    CCACGTTGTCGCGCGCACCATATCGTAGCACCTAGTGATAATAATAAGGAGGAATTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2073 [new locus tag: SAUSA300_RS11415 ]
  • symbol: Tdk
  • description: thymidine kinase
  • length: 199
  • theoretical pI: 5.89459
  • theoretical MW: 22214.2
  • GRAVY: -0.264322

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.1.21?  ExPASy
    Thymidine kinase ATP + thymidine = ADP + thymidine 5'-phosphate
  • TIGRFAM:
    Cellular processes Cellular processes Chemotaxis and motility flagellar motor switch protein FliG (TIGR00207; HMM-score: 14.4)
  • TheSEED  :
    • Thymidine kinase (EC 2.7.1.21)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Folate Biosynthesis  Thymidine kinase (EC 2.7.1.21)
    and 1 more
    Nucleosides and Nucleotides Pyrimidines pyrimidine conversions  Thymidine kinase (EC 2.7.1.21)
  • PFAM:
    P-loop_NTPase (CL0023) TK; Thymidine kinase (PF00265; HMM-score: 196.6)
    and 1 more
    AAA_19; AAA domain (PF13245; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004685
    • TAT(Tat/SPI): 0.000712
    • LIPO(Sec/SPII): 0.00219
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKPAIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVEKLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLINGKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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