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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004208000
- symbol?: —
- synonym:
- description?: collagenase-like protease
- collagenase-like protease
- U32 family peptidase
- peptidase, U32 family
- peptidase U32 family protein
- peptidase U32
- peptidase, U32 family large subunit (C1)
- peptidase family U32 family protein
- protease
- proteinase
- putative peptidase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4460536..4461804
- synteny block?: BlockID0032360
- occurrence?: in 100% of 34 strains
trhP1 (yrrO) : tRNA uridine(34) hydroxylation protein P1 [1]
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. TrhP1 is a prephanate-dependent hydroxylation protein that, in combination with TrhP2, hydroxylates uridines at tRNA position 34 generating 5-hydroxyuridine ("ho5U") via a poorly-defined mechanism. Staphylococci may alternatively hydroxylate tRNA uridine(34) using an oxygen-dependent enzyme under aerobic conditions. TrhP1-TrhP2 is the first step of a two-step pathway resulting in sequential hydroxylation (TrhP1-TrhP2) and methoxylation (TrmR) steps targeting uridine(34). Staphylococcal tRNAs requiring this modification include: tRNAGUCC, tRNAVUAC, tRNAPUGG, tRNAAUGC, tRNAL3UAG, tRNATUGU, tRNASUGA.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1570
08BA02176:
C248_1654
11819-97:
MS7_1628
6850:
RSAU_001474
71193:
ST398NM01_1676
ECT-R 2:
ECTR2_1461
ED133:
SAOV_1610
ED98:
SAAV_1604
HO 5096 0412:
SAEMRSA15_15300
JH1:
SaurJH1_1703
JH9:
SaurJH9_1669
JKD6008:
SAA6008_01582
JKD6159:
SAA6159_01545
JSNZ:
JSNZ_001601
LGA251:
SARLGA251_15150
M013:
M013TW_1631
MRSA252:
SAR1691
MSHR1132:
SAMSHR1132_14490
MSSA476:
SAS1548
Mu3:
SAHV_1599
Mu50:
SAV1612
MW2:
MW1562
RF122:
SAB1483c
ST398:
SAPIG1676
T0131:
SAT0131_01709 (fus)
TCH60:
HMPREF0772_11529
TW20:
SATW20_16070
USA300_TCH1516:
USA300HOU_1612
VC40:
SAVC_07310
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLGGQEYGLRSNADNFTME
N315 MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLGGQEYGLRSNADNFTME
NCTC8325 MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLGGQEYGLRSNADNFTME
Newman MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLGGQEYGLRSNADNFTME
USA300_FPR3757 MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLGGQEYGLRSNADNFTME
************************************************************
COL EIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKTGATGIIVADPLIIETCKE
N315 EIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKTGATGIIVADPLIIETCKE
NCTC8325 EIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKTGATGIIVADPLIIETCKE
Newman EIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKTGATGIIVADPLIIETCKE
USA300_FPR3757 EIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKTGATGIIVADPLIIETCKE
************************************************************
COL VAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGAMEMREMKEKVDIEIEAFIHG
N315 VAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGAMEMREMKEKVDIEIEAFIHG
NCTC8325 VAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGAMEMREMKEKVDIEIEAFIHG
Newman VAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGAMEMREMKEKVDIEIEAFIHG
USA300_FPR3757 VAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGAMEMREMKEKVDIEIEAFIHG
************************************************************
COL AMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLEVDDNGELDVFYNQGEVTPFAMS
N315 AMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLEVDDNGELDVFYNQGEVTPFAMS
NCTC8325 AMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLEVDDNGELDVFYNQGEVTPFAMS
Newman AMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLEVDDNGELDVFYNQGEVTPFAMS
USA300_FPR3757 AMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLEVDDNGELDVFYNQGEVTPFAMS
************************************************************
COL PKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATVVSVYRKVIDAYAADPDNFKINPEW
N315 PKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATVVSVYRKVIDAYAADPDNFKINPEW
NCTC8325 PKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATVVSVYRKVIDAYAADPDNFKINPEW
Newman PKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATVVSVYRKVIDAYAADPDNFKINPEW
USA300_FPR3757 PKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATVVSVYRKVIDAYAADPDNFKINPEW
************************************************************
COL LIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSKKSSFDFCGLVLDYNEDTKIATIQQRN
N315 LIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSKKSPFDFCGLVLDYNEDTKIATIQQRN
NCTC8325 LIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSKKSPFDFCGLVLDYNEDTKIATIQQRN
Newman LIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSKKSPFDFCGLVLDYNEDTKIATIQQRN
USA300_FPR3757 LIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSKKSPFDFCGLVLDYNEDTKIATIQQRN
************************************.***********************
COL NFKPGQEIEFFGPEIETFTQVVEAIYDEEGNSLDAARHPLQIVQIKVDRPIYPNNMMRKE
N315 NFKPGQEIEFFGPEIETFTQVVEAIYDEEGNSLDAARHPLQIVQIKVDRPIYPNNMMRKE
NCTC8325 NFKPGQEIEFFGPEIETFTQVVEAIYDEEGNSLDAARHPLQIVQIKVDRPIYPNNMMRKE
Newman NFKPGQEIEFFGPEIETFTQVVEAIYDEEGNSLDAARHPLQIVQIKVDRPIYPNNMMRKE
USA300_FPR3757 NFKPGQEIEFFGPEIETFTQVVEAIYDEEGNSLDAARHPLQIVQIKVDRPIYPNNMMRKE
************************************************************
COL IG
N315 IG
NCTC8325 IG
Newman IG
USA300_FPR3757 IG
**
- ↑ Yusuke Sakai, Satoshi Kimura, Tsutomu Suzuki
Dual pathways of tRNA hydroxylation ensure efficient translation by expanding decoding capability.
Nat Commun: 2019, 10(1);2858
[PubMed:31253794] [WorldCat.org] [DOI] (I e)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)Charles T Lauhon
Identification and Characterization of Genes Required for 5-Hydroxyuridine Synthesis in Bacillus subtilis and Escherichia coli tRNA.
J Bacteriol: 2019, 201(20);
[PubMed:31358606] [WorldCat.org] [DOI] (I e)