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⊟Summary[edit | edit source]
- pan ID?: SAUPAN006446000
- symbol?: —
- synonym:
- description?: sulfurtransferase
- sulfurtransferase
- rhodanese-related sulfurtransferase
- rhodanese-like domain protein
- protein_2622
- putative rhodanese-related sulfurtransferase
- Rhodanese domain protein
- rhodanese domain sulfurtransferase
- rhodanese family protein
- rhodanese-like domain-containing protein
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 6490516..6491472
- synteny block?: BlockID0051530
- occurrence?: in 100% of 34 strains
trhO (ybfQ) : aerobic tRNA uridine(34) hydroxylation protein TrhO [1]
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. TrhO is an oxygen-dependent hydroxylation protein that hydroxylates uridines at tRNA position 34 generating 5-hydroxyuridine ("ho5U"). Staphylococci may alternatively hydroxylate tRNA uridine(34) using an prephenate-dependent enzyme pair (TrhP1-TrhP2) under anaerobic conditions. TrhO is the first step of a two-step pathway resulting in sequential hydroxylation (TrhP1-TrhP2 or TrhO) and methoxylation (TrmR) steps targeting uridine(34). Staphylococcal tRNAs requiring this modification include: tRNAGUCC, tRNAVUAC, tRNAPUGG, tRNAAUGC, tRNAL3UAG, tRNATUGU, tRNASUGA.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2629
08BA02176:
C248_2753
11819-97:
MS7_2695
6850:
RSAU_002534
71193:
ST398NM01_2735
ECT-R 2:
ECTR2_2542
ED133:
SAOV_2735
ED98:
SAAV_2763
HO 5096 0412:
SAEMRSA15_25900
JH1:
SaurJH1_2770
JH9:
SaurJH9_2713
JKD6008:
SAA6008_02748
JKD6159:
SAA6159_02586
JSNZ:
JSNZ_002678
LGA251:
SARLGA251_24570
M013:
M013TW_2672
MRSA252:
SAR2771
MSHR1132:
SAMSHR1132_25180
MSSA476:
SAS2575
Mu3:
SAHV_2673
Mu50:
SAV2689
MW2:
MW2609
RF122:
SAB2565
ST398:
SAPIG2735
T0131:
SAT0131_02968
TCH60:
HMPREF0772_10497
TW20:
SATW20_28260
USA300_TCH1516:
USA300HOU_2691
VC40:
SAVC_12320
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGINGTLSGTKEETEQYMA
N315 MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGINGTLSGTKEETEQYMA
NCTC8325 MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGINGTLSGTKEETEQYMA
Newman MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGINGTLSGTKEETEQYMA
USA300_FPR3757 MNYQVLLYYKYMTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGINGTLSGTKEETEQYMA
*********** ************************************************
COL HMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDDVDPRHTTGQYLSPVEFRK
N315 HMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDDVDPRHTTGQYLSPVEFRK
NCTC8325 HMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDDVDPRHTTGQYLSPVEFRK
Newman HMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDDVDPRHTTGQYLSPVEFRK
USA300_FPR3757 HMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDDVDPRHTTGQYLSPVEFRK
************************************************************
COL ALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDWIKENKALFADKKVVTYCTGG
N315 ALEDDDTVIIDARNDYEFDLGHFRGAIRPDITRFRDLPDWIKENKALFTDKKVVTYCTGG
NCTC8325 ALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDWIKENKALFADKKVVTYCTGG
Newman ALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDWIKENKALFADKKVVTYCTGG
USA300_FPR3757 ALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDWIKENKALFADKKVVTYCTGG
*****************************:******************:***********
COL IRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKSEYWDGKMYVFDDRISVDINQVEKT
N315 IRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGQYWDGKMYVFDDRISVDINQVEKT
NCTC8325 IRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEYWDGKMYVFDDRISVDINQVEKT
Newman IRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEYWDGKMYVFDDRISVDINQVEKT
USA300_FPR3757 IRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEYWDGKMYVFDDRISVDINQVEKT
***********************************.:***********************
COL IIGKDWFDGKPCERYINCANPECNKQILVSEENETKYLGACSYECAKHERNRYVQANNIS
N315 IIGKDWFDGKPCERYINCANPECNKQILVSEENETKYLGACSYECAKHERNRYVQANNIS
NCTC8325 IIGKDWFDGKPCERYINCANPECNKQILVSEENETKYLGACSYECAKHERNRYVQANNIS
Newman IIGKDWFDGKPCERYINCANPECNKQILVSEENETKYLGACSYECAKHERNRYVQANNIS
USA300_FPR3757 IIGKDWFDGKPCERYINCANPECNKQILVSEENETKYLGACSYECAKHERNRYVQANNIS
************************************************************
COL DNEWQQRLTNFDDLHQHA
N315 DNEWQQRLTNFDDLHQHA
NCTC8325 DNEWQQRLTNFDDLHQHA
Newman DNEWQQRLTNFDDLHQHA
USA300_FPR3757 DNEWQQRLTNFDDLHQHA
******************
- ↑ Yusuke Sakai, Satoshi Kimura, Tsutomu Suzuki
Dual pathways of tRNA hydroxylation ensure efficient translation by expanding decoding capability.
Nat Commun: 2019, 10(1);2858
[PubMed:31253794] [WorldCat.org] [DOI] (I e)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)Charles T Lauhon
Identification and Characterization of Genes Required for 5-Hydroxyuridine Synthesis in Bacillus subtilis and Escherichia coli tRNA.
J Bacteriol: 2019, 201(20);
[PubMed:31358606] [WorldCat.org] [DOI] (I e)