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⊟Summary[edit | edit source]
- pan ID?: SAUPAN004210000
- symbol?: —
- synonym:
- description?: O-methyltransferase
- O-methyltransferase
- O-methyltransferase family protein
- methyltransferase domain-containing protein
- O-methyltransferase, predicted
- caffeoyl-CoA O-methyltransferase
- methyltransferase domain protein
- putative O-methyltransferase
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4462742..4463380
- synteny block?: BlockID0032380
- occurrence?: in 100% of 34 strains
trmR : tRNA 5-hydroxyuridine(34) O-methyltransferase [1]
• A crystal structure is available : 3NTV
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. TrmR is an O-methyltransferase specific for 5-hydroxyuridines at tRNA position 34 generating 5-methoxyuridine ("mo5U" or "5"). TrmR is the second step of a two-step pathway resulting in sequential hydroxylation (TrhP1-TrhP2) and methoxylation (TrmR) steps targeting uridine(34). Staphylococcal tRNAs requiring this modification include: tRNAGUCC, tRNAVUAC, tRNAPUGG, tRNAAUGC, tRNAL3UAG, tRNATUGU, tRNASUGA.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1572
08BA02176:
C248_1656
11819-97:
MS7_1630
6850:
RSAU_001476
71193:
ST398NM01_1678
ECT-R 2:
ECTR2_1463
ED133:
SAOV_1612
ED98:
SAAV_1606
HO 5096 0412:
SAEMRSA15_15320
JH1:
SaurJH1_1705
JH9:
SaurJH9_1671
JKD6008:
SAA6008_01584
JKD6159:
SAA6159_01547
JSNZ:
JSNZ_001603
LGA251:
SARLGA251_15170
M013:
M013TW_1633
MRSA252:
SAR1693
MSHR1132:
SAMSHR1132_14510
MSSA476:
SAS1550
Mu3:
SAHV_1601
Mu50:
SAV1614
MW2:
MW1564
RF122:
SAB1485c
ST398:
SAPIG1678
T0131:
SAT0131_01711
TCH60:
HMPREF0772_11527
TW20:
SATW20_16090
USA300_TCH1516:
USA300HOU_1614
VC40:
SAVC_07320
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIKQLIRMNNVKNILEIGT
N315 MDDLNKKYLIDLHQHQNSSIEVLREFAEGNEVPIVDRLTLDLIKQLIRMNNVKNILEIGT
NCTC8325 MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIKQLIRMNNVKNILEIGT
Newman MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIKQLIRMNNVKNILEIGT
USA300_FPR3757 MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIKQLIRMNNVKNILEIGT
**************************** *******************************
COL AIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFENQVRIIEGNALEQFENVND
N315 AIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFENQVRIIEGNALEQFENVND
NCTC8325 AIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFENQVRIIEGNALEQFENVND
Newman AIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFENQVRIIEGNALEQFENVND
USA300_FPR3757 AIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFENQVRIIEGNALEQFENVND
************************************************************
COL KVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYHGFVSDIGIVRSRNVRQMVKK
N315 KVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYHGFVSDIGIVRSRNVRQMVKK
NCTC8325 KVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYHGFVSDIGIVRSRNVRQMVKK
Newman KVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYHGFVSDIGIVRSRNVRQMVKK
USA300_FPR3757 KVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYHGFVSDIGIVRSRNVRQMVKK
************************************************************
COL VQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE
N315 VQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE
NCTC8325 VQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE
Newman VQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE
USA300_FPR3757 VQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE
********************************
- ↑ Huijeong Ryu, Tyler L Grove, Steven C Almo, Jungwook Kim
Identification of a novel tRNA wobble uridine modifying activity in the biosynthesis of 5-methoxyuridine.
Nucleic Acids Res: 2018, 46(17);9160-9169
[PubMed:29982645] [WorldCat.org] [DOI] (I p)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)