From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02549
  • pan locus tag?: SAUPAN005740000
  • symbol: SAOUHSC_02549
  • pan gene symbol?: modA
  • synonym:
  • product: molybdenum ABC transporter substrate-binding protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02549
  • symbol: SAOUHSC_02549
  • product: molybdenum ABC transporter substrate-binding protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2343695..2344477
  • length: 783
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTGAAACGTTTATTTGCTGTTGTGATTGCAATGCTTTTAGTATTAGCTGGTTGC
    TCTAATTCTAACGATAATAATGAAAGTAAAAAAGATGACGCAGACAATGGTAAGAAACAA
    GAGATTCAAGTTGCAGCGGCAGCAAGTTTAACAGATGTAACCAAGAAATTAGCTTCAGAA
    TTTAAAAAAGAGCATAAAAATGCTGATATTAAATTTAACTATGGTGGATCAGGGGCATTA
    AGAAAACAAATTGAATCAGGCGCACCTGTTGACGTATTTATGTCTGCAAATACTAAAGAT
    GTAGATGCATTAAAAGACAAGAATAAAGCGCATGATACATATAAATATGCGAAAAATAGT
    CTAGTATTAATTGGTGATAAAGATTCAAATTACACTTCAGTAAAAGACTTAAAAGACAAT
    GATAAATTAGCATTAGGTGAAGTGAAAACTGTACCAGCAGGAAAATATGCGAAACAGTAT
    TTAGATAACAATAACTTATTTAAAGAAGTCGAAAGTAAAATCGTTTATGCTAAAGATGTA
    AAACAAGTATTAAATTATGTTGAAAAGGGTAATGCGAAACAAGGTTTTGTGTATAAAACT
    GACTTATATAAACAAAATAAAAAAATTGATACTGTAAAAGTAATTAAAGAAGTAGAACTT
    AAGAAGCCAATCACATACGAAGCTGGTGCTACATCAGATAGTAAATTAGCAAAAGAGTGG
    ATGGAATTCTTAAAATCAGATAAAGCTAAAGAAATACTAAAAGAATACCACTTTGCAGCA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    783

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02549
  • symbol: SAOUHSC_02549
  • description: molybdenum ABC transporter substrate-binding protein
  • length: 260
  • theoretical pI: 9.79443
  • theoretical MW: 29051
  • GRAVY: -0.637308

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 184.5)
    and 3 more
    Metabolism Transport and binding proteins Anions phosphate binding protein (TIGR02136; HMM-score: 35)
    tungstate ABC transporter binding protein WtpA (TIGR03730; HMM-score: 34.9)
    Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 15)
  • TheSEED  :
    • Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
  • PFAM:
    PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 141.2)
    and 5 more
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 53.3)
    PBP_like_2; PBP superfamily domain (PF12849; HMM-score: 25.3)
    no clan defined Pilus_CpaD; Pilus biogenesis CpaD protein (pilus_cpaD) (PF09476; HMM-score: 17.3)
    LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 13)
    no clan defined Tox-REase-7; Restriction endonuclease fold toxin 7 (PF15649; HMM-score: 7.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LVLAGCS
  • SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.00027
    • TAT(Tat/SPI): 0.00004
    • LIPO(Sec/SPII): 0.999568
    • Cleavage Site: CS pos: 19-20. LAG-CS. Pr: 1.0000
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQVAAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVDALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQYLDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEVELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_02549&oldid=66359"