PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2230 [new locus tag: SAUSA300_RS12315 ]
• pan locus tag^?: SAUPAN005740000
• symbol: modA
• pan gene symbol^?: modA
• synonym:
• product: molybdenum ABC transporter molybdenum-binding protein ModA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2230 [new locus tag: SAUSA300_RS12315 ]
• symbol: modA
• product: molybdenum ABC transporter molybdenum-binding protein ModA
• replicon: chromosome
• strand: -
• coordinates: 2396907..2397689
• length: 783
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915207 NCBI
• RefSeq: YP_494865 NCBI
• BioCyc: see SAUSA300_RS12315
• MicrobesOnline: 1293745 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  
  ATGAAATTGAAACGTTTATTTGCTGTTGTGATTGCAATGCTTTTAGTATTAGCTGGTTGC
  TCTAATTCTAACGATAATAATGAAAGTAAAAAAGATGACGCAGACAATGGTAAGAAACAA
  GAGATTCAAGTTGCAGCGGCAGCAAGTTTAACAGATGTAACCAAGAAATTAGCTTCAGAA
  TTTAAAAAAGAGCATAAAAATGCTGATATTAAATTTAACTATGGTGGATCAGGGGCATTA
  AGAAAACAAATTGAATCAGGCGCACCTGTTGACGTATTTATGTCTGCAAATACTAAAGAT
  GTAGATGCATTAAAAGACAAGAATAAAGCGCATGATACATATAAATATGCGAAAAATAGT
  CTAGTATTAATTGGTGATAAAGATTCAAATTACACTTCAGTAAAAGACTTAAAAGACAAT
  GATAAATTAGCATTAGGTGAAGTGAAAACTGTACCAGCAGGAAAATATGCGAAACAGTAT
  TTAGATAACAATAACTTATTTAAAGAAGTCGAAAGTAAAATCGTTTATGCTAAAGATGTA
  AAACAAGTATTAAATTATGTTGAAAAGGGTAATGCGAAACAAGGTTTTGTGTATAAAACT
  GACTTATATAAACAAAATAAAAAAATTGATACTGTAAAAGTAATTAAAGAAGTAGAACTT
  AAGAAGCCAATCACATACGAAGCTGGTGCTACATCAGATAGTAAATTAGCAAAAGAGTGG
  ATGGAATTCTTAAAATCAGATAAAGCTAAAGAAATACTAAAAGAATACCACTTTGCAGCA
  TAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  783
  

⊟Protein

⊟General

• locus tag: SAUSA300_2230 [new locus tag: SAUSA300_RS12315 ]
• symbol: ModA
• description: molybdenum ABC transporter molybdenum-binding protein ModA
• length: 260
• theoretical pI: 9.79443
• theoretical MW: 29051
• GRAVY: -0.637308

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 184.5)
  and 3 more
  Metabolism Transport and binding proteins Anions phosphate binding protein (TIGR02136; HMM-score: 35)
  tungstate ABC transporter binding protein WtpA (TIGR03730; HMM-score: 34.9)
  Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 15)
• TheSEED  :
  • Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
• PFAM:
  PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 141.2)
  and 5 more
  SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 53.3)
  PBP_like_2; PBP superfamily domain (PF12849; HMM-score: 25.3)
  no clan defined Pilus_CpaD; Pilus biogenesis CpaD protein (pilus_cpaD) (PF09476; HMM-score: 17.3)
  LppaM (CL0421) LPAM_1; Prokaryotic membrane lipoprotein lipid attachment site (PF08139; HMM-score: 13)
  no clan defined Tox-REase-7; Restriction endonuclease fold toxin 7 (PF15649; HMM-score: 7.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: -0.33
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: LVLAGCS
• SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
  • SP(Sec/SPI): 0.00027
  • TAT(Tat/SPI): 0.00004
  • LIPO(Sec/SPII): 0.999568
  • Cleavage Site: CS pos: 19-20. LAG-CS. Pr: 1.0000
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160588 NCBI
• RefSeq: YP_494865 NCBI
• UniProt: A0A0H2XGY9 UniProt

⊟Protein sequence

• MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQVAAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVDALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQYLDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEVELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: modC < modB < modA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications