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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2272 [new locus tag: SACOL_RS11945 ]
  • pan locus tag?: SAUPAN005740000
  • symbol: modA
  • pan gene symbol?: modA
  • synonym:
  • product: molybdenum ABC transporter molybdenum-binding protein ModA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2272 [new locus tag: SACOL_RS11945 ]
  • symbol: modA
  • product: molybdenum ABC transporter molybdenum-binding protein ModA
  • replicon: chromosome
  • strand: -
  • coordinates: 2333040..2333822
  • length: 783
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGAAATTGAAACGTTTATTTGCTGTTGTGATTGCAATGCTTTTAGTATTAGCTGGTTGC
    TCTAATTCTAACGATAATAATGAAAGTAAAAAAGATGACGCAGACAATGGTAAGAAACAA
    GAGATTCAAGTTGCAGCGGCAGCAAGTTTAACAGATGTAACCAAGAAATTAGCTTCAGAA
    TTTAAAAAAGAGCATAAAAATGCTGATATTAAATTTAACTATGGTGGATCAGGGGCATTA
    AGAAAACAAATTGAATCAGGCGCACCTGTTGACGTATTTATGTCTGCAAATACTAAAGAT
    GTAGATGCATTAAAAGACAAGAATAAAGCGCATGATACATATAAATATGCGAAAAATAGT
    CTAGTATTAATTGGTGATAAAGATTCAAATTACACTTCAGTAAAAGACTTAAAAGACAAT
    GATAAATTAGCATTAGGTGAAGTGAAAACTGTACCAGCAGGAAAATATGCGAAACAGTAT
    TTAGATAACAATAACTTATTTAAAGAAGTCGAAAGTAAAATCGTTTATGCTAAAGATGTA
    AAACAAGTATTAAATTATGTTGAAAAGGGTAATGCGAAACAAGGTTTTGTGTATAAAACT
    GACTTATATAAACAAAATAAAAAAATTGATACTGTAAAAGTAATTAAAGAAGTAGAACTT
    AAGAAGCCAATCACATACGAAGCTGGTGCTACATCAGATAGTAAATTAGCAAAAGAGTGG
    ATGGAATTCTTAAAATCAGATAAAGCTAAAGAAATACTAAAAGAATACCACTTTGCAGCA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    783

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2272 [new locus tag: SACOL_RS11945 ]
  • symbol: ModA
  • description: molybdenum ABC transporter molybdenum-binding protein ModA
  • length: 260
  • theoretical pI: 9.79443
  • theoretical MW: 29051
  • GRAVY: -0.637308

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Metabolism Transport and binding proteins Anions molybdate ABC transporter, periplasmic molybdate-binding protein (TIGR01256; HMM-score: 184.5)
    and 3 more
    Metabolism Transport and binding proteins Anions phosphate binding protein (TIGR02136; HMM-score: 35)
    tungstate ABC transporter binding protein WtpA (TIGR03730; HMM-score: 34.9)
    Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 15)
  • TheSEED  :
    • Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1)
  • ⊞PFAM:
    PBP (CL0177) SBP_bac_11; Bacterial extracellular solute-binding protein (PF13531; HMM-score: 174.3)
    and 8 more
    SBP_bac_1; Bacterial extracellular solute-binding protein (PF01547; HMM-score: 73.5)
    Phosphonate-bd; ABC transporter, phosphonate, periplasmic substrate-binding protein (PF12974; HMM-score: 28.1)
    PBP_like_2; PBP superfamily domain (PF12849; HMM-score: 21.8)
    no clan defined DUF7537; Family of unknown function (DUF7537) (PF24381; HMM-score: 18.3)
    Pilus_CpaD; Pilus biogenesis CpaD protein (pilus_cpaD) (PF09476; HMM-score: 16)
    DUF6491; Family of unknown function (DUF6491) (PF20101; HMM-score: 14.9)
    DUF1310; Protein of unknown function (DUF1310) (PF07006; HMM-score: 10.9)
    HOATZ-like; Cilia- and flagella-associated protein HOATZ-like (PF17664; HMM-score: 10.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 3.33
    • Cellwall Score: 3.33
    • Extracellular Score: 3.33
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.8428
    • Cell wall & surface Score: 0.0119
    • Extracellular Score: 0.1453
  • ⊞LocateP: Lipid anchored
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: -0.33
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: LVLAGCS
  • ⊞SignalP: Signal peptide LIPO(Sec/SPII) length 19 aa
    • SP(Sec/SPI): 0.00027
    • TAT(Tat/SPI): 0.00004
    • LIPO(Sec/SPII): 0.999568
    • Cleavage Site: CS pos: 19-20. LAG-CS. Pr: 1.0000
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKLKRLFAVVIAMLLVLAGCSNSNDNNESKKDDADNGKKQEIQVAAAASLTDVTKKLASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVDALKDKNKAHDTYKYAKNSLVLIGDKDSNYTSVKDLKDNDKLALGEVKTVPAGKYAKQYLDNNNLFKEVESKIVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEVELKKPITYEAGATSDSKLAKEWMEFLKSDKAKEILKEYHFAA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Lipoprotein [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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