PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2229 [new locus tag: SAUSA300_RS12310 ]
• pan locus tag^?: SAUPAN005739000
• symbol: modB
• pan gene symbol^?: modB
• synonym:
• product: molybdenum ABC transporter permease ModB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2229 [new locus tag: SAUSA300_RS12310 ]
• symbol: modB
• product: molybdenum ABC transporter permease ModB
• replicon: chromosome
• strand: -
• coordinates: 2396222..2396893
• length: 672
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914656 NCBI
• RefSeq: YP_494864 NCBI
• BioCyc: see SAUSA300_RS12310
• MicrobesOnline: 1293744 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCCTGACTTAACACCTTTTTGGATATCAATACGAGTTGCTGTAATCAGTACGATTATT
  GTAACGGTTTTAGGTATTTTTATATCTAAATGGTTGTATCGTCGTAAGGGTTCGTGGGTT
  AAATTATTGGAAAGTTTATTGATATTACCTATTGTTTTGCCGCCAACGGTATTAGGTTTT
  ATTCTATTAATCATCTTCTCGCCAAGAGGACCAATCGGTCAATTCTTTGCGAATGTACTA
  CATTTACCTGTAGTGTTCACTTTGACAGGTGCTGTGATAGCATCTGTCATTGTTAGTTTT
  CCACTAATGTATCAACATACTGTGCAAGGCTTCAGAGGTATAGACACGAAAATGATTAAT
  ACAGCTAGAACGATGGGAGCAAGTGAAACGAAAATTTTCCTCAAATTAATTTTACCATTA
  GCTAAACGCTCTATTTTAGCAGGTATAATGATGAGTTTTGCTCGTGCATTAGGTGAGTTT
  GGTGCTACATTAATGGTTGCAGGATATATTCCAAATAAAACGAATACACTACCTTTAGAA
  ATATACTTCTTAGTGGAACAAGGTAGAGAAAATGAAGCGTGGTTATGGGTATTAGTGCTA
  GTCGCATTCTCTATTGTGGTTATATCTACAATTAATTTATTGAATAAAGATAAATATAAG
  GAGGTCGACTAG
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⊟Protein

⊟General

• locus tag: SAUSA300_2229 [new locus tag: SAUSA300_RS12310 ]
• symbol: ModB
• description: molybdenum ABC transporter permease ModB
• length: 223
• theoretical pI: 10.4601
• theoretical MW: 24876.8
• GRAVY: 0.797758

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions molybdate ABC transporter, permease protein (TIGR02141; HMM-score: 261.2)
  and 11 more
  NifC-like ABC-type porter (TIGR01581; HMM-score: 155.6)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein (TIGR00969; HMM-score: 136.7)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 123.5)
  Metabolism Transport and binding proteins Anions sulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 108.6)
  Metabolism Transport and binding proteins Amino acids, peptides and amines putative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 64.9)
  2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 61.5)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 50.7)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 50)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 49.3)
  Metabolism Transport and binding proteins Other thiamine/thiamine pyrophosphate ABC transporter, permease protein (TIGR01253; HMM-score: 29.2)
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 12.5)
• TheSEED  :
  • Molybdenum transport system permease protein ModB (TC 3.A.1.8.1)
  Cofactors, Vitamins, Prosthetic Groups, Pigments Folate and pterines Molybdenum cofactor biosynthesis  Molybdenum transport system permease protein ModB (TC 3.A.1.8.1)
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 68.3)
  and 2 more
  no clan defined SieB; Super-infection exclusion protein B (PF14163; HMM-score: 9.7)
  VSA (CL0656) Stevor; Subtelomeric Variable Open Reading frame (PF17410; HMM-score: 8.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001515
  • TAT(Tat/SPI): 0.000156
  • LIPO(Sec/SPII): 0.005015
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 87161820 NCBI
• RefSeq: YP_494864 NCBI
• UniProt: A0A0H2XHG9 UniProt

⊟Protein sequence

• MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKLLESLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPLMYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEFGATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDKYKEVD

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: modC < modB < modA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications