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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02284
  • pan locus tag?: SAUPAN005306000
  • symbol: SAOUHSC_02284
  • pan gene symbol?: ilvC
  • synonym:
  • product: ketol-acid reductoisomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02284
  • symbol: SAOUHSC_02284
  • product: ketol-acid reductoisomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 2115682..2116686
  • length: 1005
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAACAGTTTATTATGATCAAGATGTAAAAACGGACGCTTTACAAGGCAAAAAAATT
    GCAGTAGTAGGTTATGGATCACAAGGTCACGCGCATGCACAAAACTTAAAAGACAATGGA
    TATGATGTAGTCATCGGCATTCGCCCAGGTCGTTCTTTTGACAAAGCTAAAGAAGATGGA
    TTTGATGTGTTCCCTGTTGCAGAAGCAGTTAAGCAAGCTGATGTAATTATGGTGCTATTA
    CCTGATGAAATTCAAGGTGATGTATACAAAAACGAAATTGAACCAAATTTAGAAAAACAT
    AATGCGCTTGCATTTGCTCATGGCTTTAACATTCATTTTGGTGTTATTCAACCACCAGCT
    GATGTTGATGTATTTTTAGTAGCTCCTAAAGGACCGGGTCATTTAGTTAGACGTACATTT
    GTTGAAGGTTCTGCTGTACCATCACTATTTGGTATTCAACAAGGCGCTTCAGGTCAAGCA
    CGTAATATTGCTTTAAGTTATGCAAAAGGTATTGGTGCAACTCGTGCAGGTGTTATTGAA
    ACAACATTTAAAGAAGAAACTGAGACAGATTTATTTGGTGAACAAGCAGTACTTTGCGGT
    GGTGTATCGAAATTAATTCAAAGTGGCTTTGAAACATTAGTAGAAGCGGGTTATCAACCA
    GAATTAGCTTATTTTGAAGTATTACATGAAATGAAATTAATCGTTGATTTGATGTATGAA
    GGCGGTATGGAAAATGTACGTTACTCAATTTCAAATACTGCTGAATTTGGTGACTATGTT
    TCAGGACCACGTGTTATCACACCAGATGTTAAAGAAAATATGAAAGCTGTATTAACTGAT
    ATCCAAAATGGTAACTTCAGTAATCGCTTTATCGAAGACAATAAAAATGGATTCAAAGAA
    TTTTATAAATTACGCGAAGAACAACATGGTCATCAAATTGAAAAAGTTGGTCGTGAATTA
    CGCGAAATGATGCCTTTTATTAAATCTAAAAGCATTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

Protein Data Bank: 5W3K
Protein Data Bank: 6AQJ

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02284
  • symbol: SAOUHSC_02284
  • description: ketol-acid reductoisomerase
  • length: 334
  • theoretical pI: 5.04028
  • theoretical MW: 36955.6
  • GRAVY: -0.29491

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.86?  ExPASy
    Ketol-acid reductoisomerase (NADP+) (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 436.7)
    and 7 more
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 31.9)
    2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 17.5)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 15.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 15.3)
    Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 14.5)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 12.7)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 12.4)
  • TheSEED  :
    • Ketol-acid reductoisomerase (EC 1.1.1.86)
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  Ketol-acid reductoisomerase (EC 1.1.1.86)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Ketol-acid reductoisomerase (EC 1.1.1.86)
  • PFAM:
    NADP_Rossmann (CL0063) IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 250)
    6PGD_C (CL0106) IlvC; Acetohydroxy acid isomeroreductase, catalytic domain (PF01450; HMM-score: 218.6)
    and 12 more
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 31.6)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 30.6)
    AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 27.6)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 27.5)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 18.3)
    CoA_binding; CoA binding domain (PF02629; HMM-score: 16.5)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 15.6)
    CoA_binding_2; CoA binding domain (PF13380; HMM-score: 13.5)
    Chelatase (CL0043) Oxidored_nitro; Nitrogenase component 1 type Oxidoreductase (PF00148; HMM-score: 12.7)
    NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 12.7)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 12.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.059023
    • TAT(Tat/SPI): 0.00266
    • LIPO(Sec/SPII): 0.005356
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
    Direct targets of CodY in Staphylococcus aureus.
    J Bacteriol: 2010, 192(11);2861-77
    [PubMed:20363936] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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