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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1962 [new locus tag: NWMN_RS11325 ]
  • pan locus tag?: SAUPAN005306000
  • symbol: ilvC
  • pan gene symbol?: ilvC
  • synonym:
  • product: ketol-acid reductoisomerase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1962 [new locus tag: NWMN_RS11325 ]
  • symbol: ilvC
  • product: ketol-acid reductoisomerase
  • replicon: chromosome
  • strand: +
  • coordinates: 2173328..2174332
  • length: 1005
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGACAACAGTTTATTATGATCAAGATGTAAAAACGGACGCTTTACAAGGCAAAAAAATT
    GCAGTAGTAGGTTATGGATCACAAGGTCACGCGCATGCACAAAACTTAAAAGACAATGGA
    TATGATGTAGTCATCGGCATTCGCCCAGGTCGTTCTTTTGACAAAGCTAAAGAAGATGGA
    TTTGATGTGTTCCCTGTTGCAGAAGCAGTTAAGCAAGCTGATGTAATTATGGTGCTATTA
    CCTGATGAAATTCAAGGTGATGTATACAAAAACGAAATTGAACCAAATTTAGAAAAACAT
    AATGCGCTTGCATTTGCTCATGGCTTTAACATTCATTTTGGTGTTATTCAACCACCAGCT
    GATGTTGATGTATTTTTAGTAGCTCCTAAAGGACCGGGTCATTTAGTTAGACGTACATTT
    GTTGAAGGTTCTGCTGTACCATCACTATTTGGTATTCAACAAGGCGCTTCAGGTCAAGCA
    CGTAATATTGCTTTAAGTTATGCAAAAGGTATTGGTGCAACTCGTGCAGGTGTTATTGAA
    ACAACATTTAAAGAAGAAACTGAGACAGATTTATTTGGTGAACAAGCAGTACTTTGCGGT
    GGTGTATCGAAATTAATTCAAAGTGGCTTTGAAACATTAGTAGAAGCGGGTTATCAACCA
    GAATTAGCTTATTTTGAAGTATTACATGAAATGAAATTAATCGTTGATTTGATGTATGAA
    GGCGGTATGGAAAATGTACGTTACTCAATTTCAAATACTGCTGAATTTGGTGACTATGTT
    TCAGGACCACGTGTTATCACACCAGATGTTAAAGAAAATATGAAAGCTGTATTAACTGAT
    ATCCAAAATGGTAACTTCAGTAATCGCTTTATCGAAGACAATAAAAATGGATTCAAAGAA
    TTTTATAAATTACGCGAAGAACAACATGGTCATCAAATTGAAAAAGTTGGTCGTGAATTA
    CGCGAAATGATGCCTTTTATTAAATCTAAAAGCATTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

⊟Protein[edit | edit source]

Protein Data Bank: 5W3K
Protein Data Bank: 6AQJ

⊟General[edit | edit source]

  • locus tag: NWMN_1962 [new locus tag: NWMN_RS11325 ]
  • symbol: IlvC
  • description: ketol-acid reductoisomerase
  • length: 334
  • theoretical pI: 5.04028
  • theoretical MW: 36955.6
  • GRAVY: -0.29491

⊟Function[edit | edit source]

  • reaction:
    EC 1.1.1.86?  ExPASy
    Ketol-acid reductoisomerase (NADP+) (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family ketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 436.7)
    and 7 more
    Metabolism Energy metabolism Amino acids and amines adenosylhomocysteinase (TIGR00936; EC 3.3.1.1; HMM-score: 31.9)
    2-hydroxy-3-oxopropionate reductase (TIGR01505; EC 1.1.1.60; HMM-score: 17.5)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 15.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A 2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 15.3)
    Metabolism Energy metabolism Amino acids and amines 3-hydroxyisobutyrate dehydrogenase (TIGR01692; EC 1.1.1.31; HMM-score: 14.5)
    Metabolism Energy metabolism Pentose phosphate pathway 6-phosphogluconate dehydrogenase (decarboxylating) (TIGR00872; EC 1.1.1.44; HMM-score: 12.7)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 12.4)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    NADP_Rossmann (CL0063) IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 250)
    6PGD_C (CL0106) IlvC; Acetohydroxy acid isomeroreductase, catalytic domain (PF01450; HMM-score: 218.6)
    and 12 more
    NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 31.6)
    F420_oxidored; NADP oxidoreductase coenzyme F420-dependent (PF03807; HMM-score: 30.6)
    AdoHcyase_NAD; S-adenosyl-L-homocysteine hydrolase, NAD binding domain (PF00670; HMM-score: 27.6)
    NAD_binding_2; NAD binding domain of 6-phosphogluconate dehydrogenase (PF03446; HMM-score: 27.5)
    NAD_Gly3P_dh_N; NAD-dependent glycerol-3-phosphate dehydrogenase N-terminus (PF01210; HMM-score: 18.3)
    CoA_binding; CoA binding domain (PF02629; HMM-score: 16.5)
    ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 15.6)
    CoA_binding_2; CoA binding domain (PF13380; HMM-score: 13.5)
    Chelatase (CL0043) Oxidored_nitro; Nitrogenase component 1 type Oxidoreductase (PF00148; HMM-score: 12.7)
    NADP_Rossmann (CL0063) Mur_ligase; Mur ligase family, catalytic domain (PF01225; HMM-score: 12.7)
    Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 12.7)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.059023
    • TAT(Tat/SPI): 0.00266
    • LIPO(Sec/SPII): 0.005356
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVVIGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNALAFAHGFNIHFGVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQGASGQARNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGYQPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKAVLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
    Direct targets of CodY in Staphylococcus aureus.
    J Bacteriol: 2010, 192(11);2861-77
    [PubMed:20363936] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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