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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02282
  • pan locus tag?: SAUPAN005304000
  • symbol: SAOUHSC_02282
  • pan gene symbol?: ilvB
  • synonym:
  • product: acetolactate synthase large subunit

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02282
  • symbol: SAOUHSC_02282
  • product: acetolactate synthase large subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 2113522..2115291
  • length: 1770
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCTAAAACTCAACATGAAGTAAACCAAAATATTGACCCTTTAAAAATGGCTGAATCA
    CTTGAACCTGAACAACTAAATGAAAAAACTTTAAATGATATGCGTTCAGGATCAGAAGTG
    CTAGTAGAAGCTCTACTTAAAGAAAATGTGGATTATTTATTCGGTTATCCTGGTGGTGCC
    GTACTACCTTTATATGACACGTTTTATGATGGTAAAATCAAACATATTTTAGCAAGACAC
    GAACAAGGTGCTGTTCATGCTGCAGAAGGTTATGCACGTGTATCTGGTAAAACTGGCGTC
    GTTGTAGTTACAAGCGGTCCAGGTGCAACTAATGTAATGACAGGTATTACGGATGCACAT
    TGCGACTCTTTACCTCTAGTTGTATTCACTGGACAAGTTGCTACACCAGGCATTGGTAAA
    GATGCATTCCAAGAAGCGGATATTCTATCTATGACTTCACCAATTACAAAACAAAATTAT
    CAAGTGAAACGTGTTGAAGATATCCCTAAAATCGTACACGAAGCTTTCCATGTAGCTAAT
    TCTGGACGCAAAGGTCCTGTAGTGATTGATTTTCCAAAAGATATGGGTGTTTTAGCTACA
    AATGTGGATTTATGCGACGAAATCAATATTCCAGGTTATGAAGTTGTTACAGAACCAGAA
    AATAAAGACATTGACACTTTCATCTCACTTTTAAAAGAAGCGAAAAAGCCTGTCGTATTA
    GCCGGCGCAGGTATTAATCAATCAAAATCAAATCAATTATTAACACAGTTTGTTAATAAA
    CATCAAATCCCAACAGTTACTACTTTACTAGGTCTAGGTGCTGTACCTTATGAGGATACA
    CTATTTTTAGGTATGGGAGGAATGCATGGTTCTTATGCTAGTAACATGGCATTAACTGAG
    TGTGATTTACTCATTAATTTAGGTAGCCGCTTCGATGATAGATTAGCAAGCAAACCTGAT
    GCCTTTGCACCTAACGCCAAAATTGTACATGTAGATATTGATCCTTCAGAAATCAATAAA
    GTTATTCATGTAGATTTAGGTATTATTGCAGACTGTAAAAGATTTTTAGAATGTTTAAAT
    GATAAAAATGTTGAGACTATAGAACACAGTGACTGGGTTAAACATTGTCAAAATAATAAG
    CAGAAACACCCATTTAAACTTGGTGAAGAAGATCAAGTATTTTGTAAGCCACAACAAACA
    ATCGAATATATCGGCAAAATTACAAATGGTGAAGCAATTGTTACTACAGACGTGGGACAA
    CATCAAATGTGGGCAGCTCAATTTTATCCATTTAAAAATCACGGACAATGGGTTACAAGC
    GGTGGTTTAGGAACAATGGGATTCGGTATTCCTTCGTCAATTGGTGCCAAATTAGCTAAT
    CCTGATAAAACAGTCGTATGTTTCGTCGGTGACGGTGGTTTCCAAATGACAAACCAAGAA
    ATGGCACTTTTACCCGAATATGGTTTAGATGTCAAAATCGTACTAATCAATAATGGAACA
    TTAGGTATGGTTAAACAATGGCAAGATAAGTTCTTTAATCAACGCTTCTCACACTCAGTA
    TTTAATGGTCAACCTGATTTTATGAAAATGGCAGAAGCATATGGCGTCAAAGGTTTCTTA
    ATCGATAAGCCAGAACAACTGGAAGAACAATTAGATGCAGCGTTTGCTTATCAAGGACCA
    GCTTTAATTGAGGTTCGTATTTCCCCTACTGAAGCTGTAACCCCAATGGTTCCGAGTGGC
    AAATCAAATCATGAAATGGAGGGCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1770

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02282
  • symbol: SAOUHSC_02282
  • description: acetolactate synthase large subunit
  • length: 589
  • theoretical pI: 5.02481
  • theoretical MW: 64491.1
  • GRAVY: -0.201528

⊟Function[edit | edit source]

  • reaction:
    EC 2.2.1.6?  ExPASy
    Acetolactate synthase 2 pyruvate = 2-acetolactate + CO2
  • TIGRFAM:
    Metabolism Amino acid biosynthesis Pyruvate family acetolactate synthase, large subunit, biosynthetic type (TIGR00118; EC 2.2.1.6; HMM-score: 744.1)
    and 13 more
    glyoxylate carboligase (TIGR01504; EC 4.1.1.47; HMM-score: 322.9)
    Metabolism Energy metabolism Fermentation acetolactate synthase, catabolic (TIGR02418; EC 2.2.1.6; HMM-score: 305)
    Metabolism Central intermediary metabolism Other sulfoacetaldehyde acetyltransferase (TIGR03457; EC 2.3.3.15; HMM-score: 283.9)
    Metabolism Energy metabolism Aerobic pyruvate oxidase (TIGR02720; EC 1.2.3.3; HMM-score: 279.2)
    Metabolism Energy metabolism Sugars 3,5/4-trihydroxycyclohexa-1,2-dione hydrolase (TIGR04377; EC 3.7.1.-; HMM-score: 219.3)
    Cellular processes Cellular processes Detoxification oxalyl-CoA decarboxylase (TIGR03254; EC 4.1.1.8; HMM-score: 188.8)
    indolepyruvate/phenylpyruvate decarboxylase (TIGR03394; EC 4.1.1.43,4.1.1.74; HMM-score: 122.4)
    Metabolism Central intermediary metabolism Other indolepyruvate decarboxylase (TIGR03393; EC 4.1.1.74; HMM-score: 81.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (TIGR00173; EC 2.2.1.9; HMM-score: 58.3)
    phosphonopyruvate decarboxylase (TIGR03297; EC 4.1.1.82; HMM-score: 39.7)
    sulfopyruvate decarboxylase, beta subunit (TIGR03846; EC 4.1.1.79; HMM-score: 30.9)
    2-oxoacid:acceptor oxidoreductase, beta subunit, pyruvate/2-ketoisovalerate family (TIGR02177; HMM-score: 16)
    Metabolism Energy metabolism Chemoautotrophy CO dehydrogenase/acetyl-CoA synthase complex, epsilon subunit (TIGR00315; EC 1.2.99.2; HMM-score: 12.1)
  • TheSEED  :
    • Acetolactate synthase large subunit (EC 2.2.1.6)
    Amino Acids and Derivatives Branched-chain amino acids Branched-Chain Amino Acid Biosynthesis  Acetolactate synthase large subunit (EC 2.2.1.6)
    and 1 more
    Carbohydrates Fermentation Acetoin, butanediol metabolism  Acetolactate synthase large subunit (EC 2.2.1.6)
  • PFAM:
    THDP-binding (CL0254) TPP_enzyme_N; Thiamine pyrophosphate enzyme, N-terminal TPP binding domain (PF02776; HMM-score: 195.2)
    TPP_enzyme_C; Thiamine pyrophosphate enzyme, C-terminal TPP binding domain (PF02775; HMM-score: 176.7)
    and 3 more
    FAD_DHS (CL0085) TPP_enzyme_M; Thiamine pyrophosphate enzyme, central domain (PF00205; HMM-score: 145.1)
    THDP-binding (CL0254) POR_N; Pyruvate flavodoxin/ferredoxin oxidoreductase, thiamine diP-bdg (PF01855; HMM-score: 14.8)
    XFP_N; XFP N-terminal domain (PF09364; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Mg2+, thiamine diphosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005517
    • TAT(Tat/SPI): 0.00026
    • LIPO(Sec/SPII): 0.000345
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEALLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVVVTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNYQVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTEPENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVPYEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDIDPSEINKVIHVDLGIIADCKRFLECLNDKNVETIEHSDWVKHCQNNKQKHPFKLGEEDQVFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGIPSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQDKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVRISPTEAVTPMVPSGKSNHEMEGL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY* (repression) regulon
    CodY*(TF)important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)
  4. ↑ Charlotte D Majerczyk, Paul M Dunman, Thanh T Luong, Chia Y Lee, Marat R Sadykov, Greg A Somerville, Kip Bodi, Abraham L Sonenshein
    Direct targets of CodY in Staphylococcus aureus.
    J Bacteriol: 2010, 192(11);2861-77
    [PubMed:20363936] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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