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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2389 [new locus tag: SACOL_RS12535 ]
  • pan locus tag?: SAUPAN005922000
  • symbol: SACOL2389
  • pan gene symbol?: nreC
  • synonym:
  • product: transcriptional regulatory protein DegU

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2389 [new locus tag: SACOL_RS12535 ]
  • symbol: SACOL2389
  • product: transcriptional regulatory protein DegU
  • replicon: chromosome
  • strand: -
  • coordinates: 2448232..2448885
  • length: 654
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    TTGAAAATAGTCATTGCCGATGATCACGCTGTTGTCCGTACGGGGTTCTCTATGATTTTA
    AATTATCAAAATGATATGGAAGTTGTTGCAACGGCTGCAGATGGCGTCGAAGCTTACCAA
    AAAGTAATGGAATATAAACCTGATGTGTTACTAATGGATTTAAGTATGCCACCAGGTGAG
    TCAGGTCTTATCGCTACGAGTAAAATTGCTGACAGTTTTCCTGAAACTAAAATACTAATA
    TTAACAATGTTTGATGATGAGGAGTATTTGTTCCATGTGTTGCGTAATGGTGCGAAAGGT
    TACATATTGAAAAATGCACCTGATGAACAATTATTGTTAGCTATTCGAACTGTATATAAA
    GGTGAAACATATGTAGATATGAAACTTACAACATCTTTAGTGAATGAATTTGTATCTAAT
    TCAAATCAGGACACTGCAAACACAACAGATCCTTTTAAAATCTTATCAAAACGAGAACTA
    GAAATATTGCCACTTATTGCCAAAGGTTACGGGAATAAAGAAATTGCAGAGAAATTATTT
    GTATCTGTGAAAACAGTAGAAGCACATAAGACGCATATTATGACAAAGCTTGGCTTAAAG
    AGTAAACCTGAGCTAGTTGAATATGCATTGAAAAAGAAATTATTAGAGTTTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    654

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2389 [new locus tag: SACOL_RS12535 ]
  • symbol: SACOL2389
  • description: transcriptional regulatory protein DegU
  • length: 217
  • theoretical pI: 5.39357
  • theoretical MW: 24368.2
  • GRAVY: -0.0778802

⊟Function[edit | edit source]

  • ⊞TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 55)
    and 13 more
    LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 39)
    transcriptional regulator EpsA (TIGR03020; HMM-score: 37.5)
    Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)
    Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)
    Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
    Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
    Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
    Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 31.7)
    RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 24.5)
    proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 23.5)
    Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 21.7)
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 18.8)
    Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 11.2)
  • TheSEED  :
    • Oxygen-sensing two-component transcriptional response regulator NreC
  • ⊞PFAM:
    CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 86.8)
    HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 76.9)
    and 7 more
    Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 18.5)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 18.1)
    Sigma70_ECF; ECF sigma factor (PF07638; HMM-score: 13.8)
    Pilus (CL0327) Pilin_PilX; Minor type IV pilin, PilX (PF11530; HMM-score: 12.9)
    HTH (CL0123) HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 12.9)
    no clan defined DUF3013; Protein of unknown function (DUF3013) (PF11217; HMM-score: 12.5)
    NADP_Rossmann (CL0063) 3HCDH_RFF; 3-hydroxybutyryl-CoA dehydrogenase reduced Rossmann-fold domain (PF18321; HMM-score: 12.4)

⊟Structure, modifications & cofactors[edit | edit source]

⊟Localization[edit | edit source]

  • ⊞PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9735
    • Cytoplasmic Membrane Score: 0.0098
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.0162
  • ⊞LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.010951
    • TAT(Tat/SPI): 0.000349
    • LIPO(Sec/SPII): 0.000503
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKRELEILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • ⊞regulator: NreC* (activation) regulon

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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