PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2291 [new locus tag: NWMN_RS13180 ]
• pan locus tag^?: SAUPAN005922000
• symbol: NWMN_2291
• pan gene symbol^?: nreC
• synonym:
• product: transcriptional regulator DegU family protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2291 [new locus tag: NWMN_RS13180 ]
• symbol: NWMN_2291
• product: transcriptional regulator DegU family protein
• replicon: chromosome
• strand: -
• coordinates: 2516730..2517383
• length: 654
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331405 NCBI
• RefSeq: YP_001333325 NCBI
• BioCyc:
• MicrobesOnline: 3707893 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGAAAATAGTCATTGCCGATGATCACGCTGTTGTCCGTACGGGGTTCTCTATGATTTTA
  AATTATCAAAATGATATGGAAGTTGTTGCAACGGCTGCAGATGGCGTCGAAGCTTACCAA
  AAAGTAATGGAATATAAACCTGATGTGTTACTAATGGATTTAAGTATGCCACCAGGTGAG
  TCAGGTCTTATCGCTACGAGTAAAATTGCTGACAGTTTTCCTGAAACTAAAATACTAATA
  TTAACAATGTTTGATGATGAGGAGTATTTGTTCCATGTGTTGCGTAATGGTGCGAAAGGT
  TACATATTGAAAAATGCACCTGATGAACAATTATTGTTAGCTATTCGAACTGTATATAAA
  GGTGAAACATATGTAGATATGAAACTTACAACATCTTTAGTGAATGAATTTGTATCTAAT
  TCAAATCAGGACACTGCAAACACAACAGATCCTTTTAAAATCTTATCAAAACGAGAACTA
  GAAATATTGCCACTTATTGCCAAAGGTTACGGGAATAAAGAAATTGCAGAGAAATTATTT
  GTATCTGTGAAAACAGTAGAAGCACATAAGACGCATATTATGACAAAGCTTGGCTTAAAG
  AGTAAACCTGAGCTAGTTGAATATGCATTGAAAAAGAAATTATTAGAGTTTTAG
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⊟Protein

⊟General

• locus tag: NWMN_2291 [new locus tag: NWMN_RS13180 ]
• symbol: NWMN_2291
• description: transcriptional regulator DegU family protein
• length: 217
• theoretical pI: 5.39357
• theoretical MW: 24368.2
• GRAVY: -0.0778802

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes Sporulation and germination sporulation transcription factor Spo0A (TIGR02875; HMM-score: 55)
  and 13 more
  LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 39)
  transcriptional regulator EpsA (TIGR03020; HMM-score: 37.5)
  Signal transduction Regulatory functions DNA interactions phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)
  Signal transduction Signal transduction Two-component systems phosphate regulon transcriptional regulatory protein PhoB (TIGR02154; HMM-score: 35.8)
  Metabolism Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
  Signal transduction Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
  Signal transduction Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 35.6)
  Signal transduction Regulatory functions DNA interactions PEP-CTERM-box response regulator transcription factor (TIGR02915; HMM-score: 31.7)
  RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 24.5)
  proteobacterial dedicated sortase system response regulator (TIGR03787; HMM-score: 23.5)
  Signal transduction Regulatory functions DNA interactions heavy metal response regulator (TIGR01387; HMM-score: 21.7)
  RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 18.8)
  Signal transduction Signal transduction Two-component systems TMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 11.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 88.1)
  HTH (CL0123) GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 78.1)
  and 6 more
  Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 19.4)
  HTH_23; Homeodomain-like domain (PF13384; HMM-score: 16.5)
  DUF2089; Protein of unknown function (DUF2089) (PF09862; HMM-score: 14.3)
  Sigma70_ECF; ECF sigma factor (PF07638; HMM-score: 14)
  Pilus (CL0327) Pilin_PilX; Minor type IV pilin, PilX (PF11530; HMM-score: 12.9)
  HTH (CL0123) HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 11.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: NreB (sensor histidine kinase) sensing nitrate and nitrite
• genes regulated by NreC*, TF important in Nitrate and nitrite respirationRegPrecise

  activation

  narK*

  nreC* < nreB* < nreA* < narI < narJ < narH < narG

  cysG < nirD < nirB < nirR

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010951
  • TAT(Tat/SPI): 0.000349
  • LIPO(Sec/SPII): 0.000503
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222503 NCBI
• RefSeq: YP_001333325 NCBI
• UniProt: A6QJN1 UniProt

⊟Protein sequence

• MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVMEYKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYILKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTTDPFKILSKRELEILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2291 < NWMN_2292 < NWMN_2293 < narI < narJ < narH < narG

⊟Regulation

• regulator: NreC* (activation) regulon

  NreC*

  (TF)

  important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications