PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_2292 [new locus tag: NWMN_RS13185 ]
• pan locus tag^?: SAUPAN005923000
• symbol: NWMN_2292
• pan gene symbol^?: nreB
• synonym:
• product: sensor histidine kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_2292 [new locus tag: NWMN_RS13185 ]
• symbol: NWMN_2292
• product: sensor histidine kinase
• replicon: chromosome
• strand: -
• coordinates: 2517406..2518440
• length: 1035
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331406 NCBI
• RefSeq: YP_001333326 NCBI
• BioCyc:
• MicrobesOnline: 3707894 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  1021
  
  ATGATTAATGAGGACAGTATACAGTTAGATACTTTATTGAAAAAATATTATGAGCATTCA
  ATCGAAAAGATAGTTTTTGCTGATGATAACGGTAAAATTATTGCAATGAATGATGCTGCT
  AAAGATATATTATCTGAAGAAGATAATTACAGTGCTGTGGCGAATGCGATTTGTCACAGA
  TGTGAAGGGTATACAAATGCCTATGATGTACAATCATGTAAAGACTGTTTCTTAGAATCG
  ATGCAAGTACAAGCTACGAATTTCCAAGTATTTATGAAGACGAAAGATCAAAAAGTGATG
  CCATTTACAGCAACATATCAGTTGATTGATCAAGACAGGGGAATTCATGCATTTACGCTT
  CAAAATGTTTCATCACAAATTGAACAGCAAGAGAAATTGCATCAACAACGTATGATGCGT
  AAAACAATCTCAGCCCAAGAAAATGAAAGAAAGCGAATTTCTAGAGAGTTACATGATAGT
  GTGATTCAAGAAATGCTCAATGTAGATGTTCAATTGAGATTATTAAAATATCAAGAAGAT
  ACAACTAAGCTTTTGGAAGACGCAGAAAATATTGAATATATTGTCGCTAAATTAATTGAT
  GATATACGTAATATGTCGGTTGAATTAAGACCCGCTTCTTTGGATGATCTAGGCCTTGAA
  GCAGCTTTTAAATCATATTTTAAACAGTTTGAAGAAAATTATGGAATAAAAATCATATAT
  ACATCAAATATTAAAAATACTCGCTTTGATAGTGATATTGAAACGGTTGTTTATCGAGTA
  GTGCAAGAAGCTATTTTAAATGCGTTGAAATACGCTGATGTAAATGAAATAAATGTAGGT
  ATCCGTCAAACTGGGCGCCATTTGGTAGCAGAAGTGATAGATGCGGGAAATGGTTTTGAT
  CCAAGTTCTAAGCCTAAAGGTTCTGGACTCGGTTTATATGGAATGAATGAGCGAGCTGAG
  TTAGTCAGCGGTAGCGTCAATATTGAAACGAAAATTGGTGAGGGTACCAATGTTACATTG
  AACATTCCAATTTGA
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⊟Protein

⊟General

• locus tag: NWMN_2292 [new locus tag: NWMN_RS13185 ]
• symbol: NWMN_2292
• description: sensor histidine kinase
• length: 344
• theoretical pI: 4.65774
• theoretical MW: 39120
• GRAVY: -0.409302

⊟Function

• reaction:
  EC 2.7.13.3?  ExPASy
  Histidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
• TIGRFAM:
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
  Signal transduction Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 17.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 53.4)
  and 5 more
  HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 38.7)
  HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 23.6)
  PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 22)
  UBA (CL0214) WIYLD; Ubiquitin-binding WIYLD domain (PF10440; HMM-score: 13.6)
  no clan defined DUF4974; Domain of unknown function (DUF4974) (PF16344; HMM-score: 12.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: [4Fe-4S] cluster
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 1.05
  • Cytoplasmic Membrane Score: 8.78
  • Cellwall Score: 0.08
  • Extracellular Score: 0.09
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.007402
  • TAT(Tat/SPI): 0.000636
  • LIPO(Sec/SPII): 0.000999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151222504 NCBI
• RefSeq: YP_001333326 NCBI
• UniProt: A6QJN2 UniProt

⊟Protein sequence

• MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDILSEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPFTATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDSVIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLGLEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNEINVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGEGTNVTLNIPI

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: NWMN_2291 < NWMN_2292 < NWMN_2293 < narI < narJ < narH < narG

⊟Regulation

• regulator: NreC* (activation) regulon

  NreC*

  (TF)

  important in Nitrate and nitrite respiration; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications