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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL2390 [new locus tag: SACOL_RS12540 ]
- pan locus tag?: SAUPAN005923000
- symbol: SACOL2390
- pan gene symbol?: nreB
- synonym:
- product: sensory box histidine kinase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL2390 [new locus tag: SACOL_RS12540 ]
- symbol: SACOL2390
- product: sensory box histidine kinase
- replicon: chromosome
- strand: -
- coordinates: 2448908..2449942
- length: 1035
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236207 NCBI
- RefSeq: YP_187193 NCBI
- BioCyc: see SACOL_RS12540
- MicrobesOnline: 913870 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1021ATGATTAATGAGGACAGTATACAGTTAGATACTTTATTGAAAAAATATTATGAGCATTCA
ATCGAAAAGATAGTTTTTGCTGATGATAACGGTAAAATTATTGCAATGAATGATGCTGCT
AAAGATATATTATCTGAAGAAGATAATTACAGTGCTGTGGCGAATGCGATTTGTCACAGA
TGTGAAGGGTATACAAATGCCTATGATGTACAATCATGTAAAGACTGTTTCTTAGAATCG
ATGCAAGTACAAGCTACGAATTTCCAAGTATTTATGAAGACGAAAGATCAAAAAGTGATG
CCATTTACAGCAACATATCAGTTGATTGATCAAGACAGGGGAATTCATGCATTTACGCTT
CAAAATGTTTCATCACAAATTGAACAGCAAGAGAAATTGCATCAACAACGTATGATGCGT
AAAACAATCTCAGCCCAAGAAAATGAAAGAAAGCGAATTTCTAGAGAGTTACATGATAGT
GTGATTCAAGAAATGCTCAATGTAGATGTTCAATTGAGATTATTAAAATATCAAGAAGAT
ACAACTAAGCTTTTGGAAGACGCAGAAAATATTGAATATATTGTCGCTAAATTAATTGAT
GATATACGTAATATGTCGGTTGAATTAAGACCCGCTTCTTTGGATGATCTAGGCCTTGAA
GCAGCTTTTAAATCATATTTTAAACAGTTTGAAGAAAATTATGGAATAAAAATCATATAT
ACATCAAATATTAAAAATACTCGCTTTGATAGTGATATTGAAACGGTTGTTTATCGAGTA
GTGCAAGAAGCTATTTTAAATGCGTTGAAATACGCTGATGTAAATGAAATAAATGTAGGT
ATCCGTCAAACTGGGCGCCATTTGGTAGCAGAAGTGATAGATGCGGGAAATGGTTTTGAT
CCAAGTTCTAAGCCTAAAGGTTCTGGACTCGGTTTATATGGAATGAATGAGCGAGCTGAG
TTAGTCAGCGGTAGCGTCAATATTGAAACGAAAATTGGTGAGGGTACCAATGTTACATTG
AACATTCCAATTTGA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL2390 [new locus tag: SACOL_RS12540 ]
- symbol: SACOL2390
- description: sensory box histidine kinase
- length: 344
- theoretical pI: 4.65774
- theoretical MW: 39120
- GRAVY: -0.409302
⊟Function[edit | edit source]
- reaction: EC 2.7.13.3? ExPASyHistidine kinase ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
- TIGRFAM: Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 17.2)
- TheSEED :
- Oxygen-sensing two-component system sensor histidine kinase NreB
- PFAM: His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 53.4)and 5 moreHATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 38.7)HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 23.6)PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 22)UBA (CL0214) WIYLD; Ubiquitin-binding WIYLD domain (PF10440; HMM-score: 13.6)no clan defined DUF4974; Domain of unknown function (DUF4974) (PF16344; HMM-score: 12.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors: [4Fe-4S] cluster
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 1.05
- Cytoplasmic Membrane Score: 8.78
- Cellwall Score: 0.08
- Extracellular Score: 0.09
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007402
- TAT(Tat/SPI): 0.000636
- LIPO(Sec/SPII): 0.000999
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDILSEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPFTATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDSVIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLGLEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNEINVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGEGTNVTLNIPI
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: NreC* (activation) regulon
NreC* (TF) important in Nitrate and nitrite respiration; RegPrecise transcription unit transferred from N315 data RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p)