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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002360
  • pan locus tag?: SAUPAN005923000
  • symbol: nreB
  • pan gene symbol?: nreB
  • synonym:
  • product: nitrate respiration regulation sensor histidine kinase NreB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002360
  • symbol: nreB
  • product: nitrate respiration regulation sensor histidine kinase NreB
  • replicon: chromosome
  • strand: -
  • coordinates: 2358647..2359681
  • length: 1035
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGATTAATGAGGACAGTATACAGTTAGATACTTTATTGAAAAAATATTATGAGCATTCA
    ATCGAAAAGATAGTTTTTGCTGATGATAACGGTAAAATTATTGCAATGAATGATGCTGCT
    AAAGATATATTATCTGAAGAAGATAATTACAGTGCTGTGGCGAATGCGATTTGTCACAGA
    TGTGAAGGGTATACAAATGCCTATGATGTACAATCATGTAAAGACTGTTTCTTAGAATCG
    ATGCAAGTACAAGCTACGAATTTCCAAGTATTTATGAAGACGAAAGATCAAAAAGTGATG
    CCATTTACAGCAACATATCAGTTGATTGATCAAGACAGGGGAATTCATGCATTTACGCTT
    CAAAATGTTTCATCACAAATTGAACAGCAAGAGAAATTGCATCAACAACGTATGATGCGT
    AAAACAATCTCAGCCCAAGAAAATGAAAGAAAGCGAATTTCTAGAGAGTTACATGATAGT
    GTGATTCAAGAAATGCTCAATGTAGATGTTCAATTGAGATTATTAAAATATCAAGAAGAT
    ACAACTAAGCTTTTGGAAGACGCAGAAAATATTGAATATATTGTCGCTAAATTAATTGAT
    GATATACGTAATATGTCGGTTGAATTAAGACCCGCTTCTTTGGATGATCTAGGCCTTGAA
    GCAGCTTTTAAATCATATTTTAAACAGTTTGAAGAAAATTATGGAATAAAAATCATATAT
    ACATCAAATATTAAAAATACTCGCTTTGATAGTGATATTGAAACGGTTGTTTATCGAGTA
    GTGCAAGAAGCTATTTTAAATGCGTTGAAATACGCTGATGTAAATGAAATAAATGTAGGT
    ATCCGTCAAACTGGGCGCCATTTGGTAGCAGAAGTGATAGATGCGGGAAATGGTTTTGAT
    CCAAGTTCTAAGCCTAAAGGTTCTGGACTCGGTTTATATGGAATGAATGAGCGAGCTGAG
    TTAGTCAGCGGTAGCGTCAATATTGAAACGAAAATTGGTGAGGGTACCAATGTTACATTG
    AACATTCCAATTTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1035

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002360
  • symbol: NreB
  • description: nitrate respiration regulation sensor histidine kinase NreB
  • length: 344
  • theoretical pI: 4.65774
  • theoretical MW: 39120
  • GRAVY: -0.409302

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 21.2)
    Signal transduction Regulatory functions Small molecule interactions PAS domain S-box protein (TIGR00229; HMM-score: 17.2)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    His_Kinase_A (CL0025) HisKA_3; Histidine kinase (PF07730; HMM-score: 54.5)
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 52.4)
    and 6 more
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 27.3)
    PAS_Fold (CL0183) PAS_9; PAS domain (PF13426; HMM-score: 19.8)
    Cache (CL0165) Cache_WalK; WalK sensor protein kinase Chache domain (PF23846; HMM-score: 16.7)
    Secretin_N0 (CL0751) FecR_C; FecR, C-terminal (PF16344; HMM-score: 14.4)
    UBA (CL0214) WIYLD; Ubiquitin-binding WIYLD domain (PF10440; HMM-score: 12.7)
    PAS_Fold (CL0183) PAS_8; PAS domain (PF13188; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8978
    • Cytoplasmic Membrane Score: 0.0768
    • Cell wall & surface Score: 0.0009
    • Extracellular Score: 0.0244
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007402
    • TAT(Tat/SPI): 0.000636
    • LIPO(Sec/SPII): 0.000999
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MINEDSIQLDTLLKKYYEHSIEKIVFADDNGKIIAMNDAAKDILSEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPFTATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDSVIQEMLNVDVQLRLLKYQEDTTKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLGLEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNEINVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGEGTNVTLNIPI

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC (activation) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    NreC(TF)important in Nitrate and nitrite respiration;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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