From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002364
  • pan locus tag?: SAUPAN005927000
  • symbol: narH
  • pan gene symbol?: narH
  • synonym:
  • product: nitrate reductase subunit beta

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002364
  • symbol: narH
  • product: nitrate reductase subunit beta
  • replicon: chromosome
  • strand: -
  • coordinates: 2361415..2362974
  • length: 1560
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    TTGAAGATTAAAGCGCAAGTTGCGATGGTATTAAATTTAGATAAATGCATAGGATGCCAT
    ACGTGTAGTGTGACATGTAAAAACACTTGGACAAATCGTCCAGGTGCTGAGTACATGTGG
    TTCAACAACGTAGAAACGAAGCCAGGTGTAGGGTATCCGAAACGTTGGGAAGACCAAGAA
    CACTACAAAGGTGGTTGGGTACTAAATCGTAAAGGGAAACTTGAATTAAAATCTGGAAGT
    AGAATTTCAAAAATTGCTTTAGGTAAAATTTTTTATAACCCAGATATGCCATTAATTAAA
    GATTATTATGAGCCATGGAACTATAATTATGAACATTTAACAACTGCGAAATCAGGGAAG
    CATTCGCCAGTTGCTAGAGCGTATTCAGAAATTACGGGGGATAACATTGAAATTGAATGG
    GGACCTAACTGGGAAGATGACTTAGCAGGTGGTCATGTTACAGGTCCAAAAGATCCTAAC
    ATCCAAAAAATAGAAGAAGATATTAAATTCCAATTTGACGAAACTTTTATGATGTATTTA
    CCACGTTTATGTGAACATTGCTTGAATCCAAGTTGCGTTGCATCGTGTCCTTCAGGTGCT
    ATGTATAAACGTGATGAAGATGGCATTGTATTAGTTGATCAAGACGCATGTCGTGGATGG
    CGTTACTGTATGACAGGTTGTCCATATAAGAAAGTTTACTTCAATTGGAAAACGAACAAA
    GCTGAAAAATGTACTTTCTGTTTCCCGAGAATCGAGGCGGGTATGCCTACGGTATGCTCT
    GAAACTTGTACAGGACGTATGCGTTACTTAGGTGTTTTATTATATGATGCAGATCGAGTT
    CATGAAGCGGCTTCAGCAGTAGACGAGAAAGATTTATACGAAAAACAATTAGATATATTC
    TTAAATCCATTTGACGAAGAAGTCATTGCTCAAGCTGAAAAAGATGGTATTGGTTATGAT
    TGGATTGAAGCTGCACAAAACTCTCCAATTTATAAGTTAGCAATTGAATATAAACTTGCA
    TTTCCGTTACATCCTGAATTTAGAACGATGCCGATGGTTTGGTATTGTCCACCACTTAGC
    CCAATTATGAGCTATTTTGAAGGTAAAAATACAACTCAAAATCCAGATGCGATTTTCCCA
    GCCATTGAAGAAATGCGTTTACCTATTGAATACTTAGCTAATATTTTCACTGCAGGAGAT
    ACAGAGCCAGTAAAAGGAGCCCTACAACGTATGGCAATGATGAGAAGTTATATGAGATCT
    CAAGTTACTCAACAGCCATTTGACACGTCTCGATTAGAGCGATTAGGTATAACCGAGCGC
    CAAACTAAAGACATGTATCGTTTATTAGGACTAGCTAAATATGAAGATCGATTTGTTATT
    CCAACATCACACAAAGAAACCTATTTAGATACGTATCACGCACAAGGTAGTACAGGATAC
    AATTACGGCGGTGAGCATTTTGGAGATAACTGTGAAGGCTGTGGTGTTGCAGTAGGTTCA
    GGGAAAACTGGTCAAGAAATTTATAATGAGAATTTCTATGGAGGGATTTTCCGTGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002364
  • symbol: NarH
  • description: nitrate reductase subunit beta
  • length: 519
  • theoretical pI: 5.54155
  • theoretical MW: 59430.2
  • GRAVY: -0.536609

⊟Function[edit | edit source]

  • TIGRFAM:
    Metabolism Energy metabolism Anaerobic nitrate reductase, beta subunit (TIGR01660; EC 1.7.99.4; HMM-score: 841.3)
    and 21 more
    Metabolism Energy metabolism Anaerobic DMSO reductase family type II enzyme, iron-sulfur subunit (TIGR03478; HMM-score: 288.5)
    Metabolism Energy metabolism Electron transport DMSO reductase family type II enzyme, iron-sulfur subunit (TIGR03478; HMM-score: 288.5)
    dimethylsulfoxide reductase, chain B (TIGR02951; HMM-score: 107.1)
    Metabolism Energy metabolism Electron transport cytochrome c nitrite reductase, Fe-S protein (TIGR03149; EC 1.7.2.2; HMM-score: 86.9)
    Metabolism Energy metabolism Anaerobic formate dehydrogenase, beta subunit (TIGR01582; EC 1.2.1.2; HMM-score: 71.2)
    Metabolism Energy metabolism Electron transport formate dehydrogenase, beta subunit (TIGR01582; EC 1.2.1.2; HMM-score: 71.2)
    Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 23.5)
    [FeFe] hydrogenase, group B1/B3 (TIGR04105; EC 1.12.-.-; HMM-score: 22.6)
    2-oxoacid:acceptor oxidoreductase, delta subunit, pyruvate/2-ketoisovalerate family (TIGR02179; HMM-score: 16.2)
    Metabolism Central intermediary metabolism Nitrogen fixation ferredoxin III, nif-specific (TIGR02936; HMM-score: 14.9)
    methylamine methyltransferase corrinoid protein reductive activase (TIGR04270; HMM-score: 14.8)
    Metabolism Energy metabolism Amino acids and amines choline TMA-lyase-activating enzyme (TIGR04395; EC 1.97.-.-; HMM-score: 14.5)
    Genetic information processing Protein fate Protein modification and repair glycine radical enzyme activase, YjjW family (TIGR04041; EC 1.97.1.-; HMM-score: 13.6)
    glycyl-radical enzyme activating protein (TIGR02494; EC 1.97.1.-; HMM-score: 12.2)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, dissimilatory-type beta subunit (TIGR02066; EC 1.8.99.3; HMM-score: 10.9)
    coenzyme F420 hydrogenase, subunit gamma (TIGR03294; EC 1.12.98.1; HMM-score: 9.2)
    [FeFe] hydrogenase, group A (TIGR02512; EC 1.12.-.-; HMM-score: 7.5)
    Metabolism Energy metabolism Electron transport electron transport complex, RnfABCDGE type, B subunit (TIGR01944; HMM-score: 5.8)
    Metabolism Energy metabolism Methanogenesis putative methanogenesis marker 16 metalloprotein (TIGR03287; HMM-score: 4.3)
    Metabolism Central intermediary metabolism Sulfur metabolism sulfite reductase, subunit C (TIGR02912; EC 1.8.-.-; HMM-score: 4)
    Metabolism Energy metabolism Other benzoyl-CoA oxygenase/reductase, BoxA protein (TIGR03224; EC 1.14.12.21; HMM-score: 3.9)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    4Fe-4S (CL0344) Fer4_11; 4Fe-4S dicluster domain (PF13247; HMM-score: 125.1)
    no clan defined Nitr_red_bet_C; Respiratory nitrate reductase beta C-terminal (PF14711; HMM-score: 101.5)
    and 11 more
    4Fe-4S (CL0344) Fer4_10; 4Fe-4S dicluster domain (PF13237; HMM-score: 37.5)
    Fer4_7; 4Fe-4S dicluster domain (PF12838; HMM-score: 29.1)
    Fer4_2; 4Fe-4S binding domain (PF12797; HMM-score: 19.9)
    Fer4_9; 4Fe-4S dicluster domain (PF13187; HMM-score: 17.7)
    Fer4_6; 4Fe-4S binding domain (PF12837; HMM-score: 17.1)
    Fer4_13; 4Fe-4S single cluster domain of Ferredoxin I (PF13370; HMM-score: 16.4)
    Fer4; 4Fe-4S binding domain (PF00037; HMM-score: 15)
    SUKH (CL0526) SUKH_5; SMI1-KNR4 cell-wall (PF14567; HMM-score: 12.6)
    4Fe-4S (CL0344) Fer4_8; 4Fe-4S dicluster domain (PF13183; HMM-score: 11.5)
    Fer4_21; 4Fe-4S dicluster domain (PF14697; HMM-score: 10.6)
    Fer4_17; 4Fe-4S dicluster domain (PF13534; HMM-score: 9.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.1452
    • Cytoplasmic Membrane Score: 0.5008
    • Cell wall & surface Score: 0.0002
    • Extracellular Score: 0.3538
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.193248
    • TAT(Tat/SPI): 0.002364
    • LIPO(Sec/SPII): 0.257463
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNVETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDYYEPWNYNYEHLTTAKSGKHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPNIQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACRGWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYDADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGIGYDWIEAAQNSPIYKLAIEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTTQNPDAIFPAIEEMRLPIEYLANIFTAGDTEPVKGALQRMAMMRSYMRSQVTQQPFDTSRLERLGITERQTKDMYRLLGLAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIYNENFYGGIFRD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC (activation) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    NreC(TF)important in Nitrate and nitrite respiration;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_002364&oldid=108005"