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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002363
  • pan locus tag?: SAUPAN005926000
  • symbol: narJ
  • pan gene symbol?: narJ
  • synonym:
  • product: nitrate reductase molybdenum cofactor assembly chaperone

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002363
  • symbol: narJ
  • product: nitrate reductase molybdenum cofactor assembly chaperone
  • replicon: chromosome
  • strand: -
  • coordinates: 2360847..2361422
  • length: 576
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    GTGATTAATTTCGATAATTTTAAAAAATATCAGGAAAGTTTTGGATATATGGCACAACAG
    TTATGTTTTCCAGAAAAATTAACTTTTCATCCAAAAACTTTTGAAGAAACAATTTCGAAA
    GATCATCCAGCATATGAAGATTTAGTAGCATTTAGAAATGTAATGATGACATTTTCATTA
    TCAGAAATCAAAGCGATTTATACAGATGCATTTGATTTTACGAAAAAAGCACCATTATAT
    ATGACGTATAACAAATTTGATACGCAAAAAGAACGTGGGCAAATGTTAGCTAAATTAAAG
    GTATTATATGAAATGTTTGGACTAGAAATGGTAGATAATGAACTTTCTGATTATCTACCG
    TTGATGCTTCAATTTTTGCAAGTGGCAGAATGGCGAAATGATCCAAGAGCTGAAGAGAAT
    ATTCAATTAATCATTATGATTATTGAGGATGGAACATATGTGATGGCGAATGCACTTGGT
    GAAGATAATAATCCATATGCCTATGTGATTCAAGCACTTAGAAAAACATTGAAATTATGT
    TTGACATCACCAAAAGGAGTGAAACATCATGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    576

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002363
  • symbol: NarJ
  • description: nitrate reductase molybdenum cofactor assembly chaperone
  • length: 191
  • theoretical pI: 5.32727
  • theoretical MW: 22375.8
  • GRAVY: -0.293194

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein folding and stabilization nitrate reductase molybdenum cofactor assembly chaperone (TIGR00684; HMM-score: 63.8)
    and 1 more
    DMSO reductase family type II enzyme chaperone (TIGR03482; HMM-score: 28.3)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined Nitrate_red_del; Nitrate reductase delta subunit (PF02613; HMM-score: 34.2)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9881
    • Cytoplasmic Membrane Score: 0.01
    • Cell wall & surface Score: 0
    • Extracellular Score: 0.0019
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003278
    • TAT(Tat/SPI): 0.000141
    • LIPO(Sec/SPII): 0.00077
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPAYEDLVAFRNVMMTFSLSEIKAIYTDAFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVLYEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALGEDNNPYAYVIQALRKTLKLCLTSPKGVKHHA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, NreC (activation) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [2]
    NreC(TF)important in Nitrate and nitrite respiration;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ 2.0 2.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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