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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1689 [new locus tag: SACOL_RS08615 ]
  • pan locus tag?: SAUPAN004239000
  • symbol: relA2
  • pan gene symbol?: relA
  • synonym:
  • product: GTP pyrophosphokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1689 [new locus tag: SACOL_RS08615 ]
  • symbol: relA2
  • product: GTP pyrophosphokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1718109..1720298
  • length: 2190
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAACAACGAATATCCATATAGTGCAGACGAAGTTCTTCACAAAGCAAAATCATATTTG
    TCAGCAGATGAATATGAGTATGTTTTAAAAAGCTATCATATTGCTTATGAAGCACATAAA
    GGTCAGTTCCGAAAAAACGGATTACCATACATTATGCATCCTATACAAGTTGCAGGTATT
    TTAACAGAAATGCGATTAGACGGACCGACGATTGTCGCAGGTTTTTTGCATGATGTAATT
    GAAGATACACCGTATACATTTGAAGATGTAAAAGAAATGTTCAATGAAGAAGTTGCTCGA
    ATTGTTGATGGTGTGACGAAGCTTAAAAAAGTAAAATACCGCTCAAAAGAAGAACAACAA
    GCTGAAAATCATCGCAAGTTATTTATTGCGATTGCCAAAGATGTACGCGTAATTTTGGTG
    AAATTAGCAGACAGATTACATAATATGCGTACCTTGAAAGCCATGCCGCGCGAAAAACAA
    ATTAGAATTTCTCGAGAAACATTAGAAATTTATGCACCATTAGCACATCGTCTTGGTATT
    AATACAATCAAATGGGAACTAGAAGATACGGCTCTTCGTTATATTGATAATGTGCAATAT
    TTTAGAATAGTCAATTTAATGAAGAAGAAACGTAGTGAACGTGAAGCGTATATCGAAACG
    GCTATTGATAGAATACGTACTGAAATGGACCGAATGAATATCGAAGGCGATATAAATGGT
    AGACCTAAACATATTTACAGTATTTATCGGAAAATGATGAAGCAGAAAAAACAATTTGAT
    CAAATTTTTGATTTGTTGGCGATACGTGTTATTGTCAATTCTATTAATGATTGTTATGCG
    ATACTTGGGTTGGTGCATACGTTATGGAAACCGATGCCAGGACGTTTTAAAGATTATATT
    GCAATGCCTAAACAAAATTTGTATCAGTCATTGCATACTACAGTAGTAGGCCCAAATGGA
    GACCCGCTCGAAATCCAAATACGAACGTTTGATATGCACGAAATTGCTGAGCATGGTGTT
    GCAGCACACTGGGCTTACAAAGAAGGTAAAAAAGTAAGTGAAAAAGATCAAACTTATCAA
    AATAAGTTAAATTGGTTAAAAGAATTAGCTGAAGCGGATCATACATCGTCTGACGCTCAA
    GAATTTATGGAAACCTTAAAATATGACTTACAGAGTGACAAAGTATACGCATTTACCCCA
    GCGAGTGATGTTATTGAGTTGCCATATGGTGCTGTGCCGATTGATTTTGCTTATGCGATT
    CACAGTGAAGTAGGTAATAAGATGATTGGTGCCAAGGTGAATGGCAAAATTGTACCAATT
    GACTATATTTTACAAACAGGCGATATTGTTGAAATACGTACTAGTAAACATTCATATGGA
    CCAAGTCGTGATTGGTTGAAAATTGTTAAATCGTCTAGTGCCAAAGGTAAAATTAAAAGT
    TTCTTCAAAAAACAAGATCGTTCATCTAATATTGAAAAAGGCCGAATGATGGTTGAAGCT
    GAAATAAAAGAGCAAGGATTTAGAGTCGAAGATATTTTGACAGAGAAAAATATTCAGGTT
    GTTAATGAAAAATATAACTTTGCAAATGAAGATGATTTATTCGCAGCTGTAGGATTTGGC
    GGCGTGACATCCTTACAGATTGTTAATAAATTAACTGAAAGACAACGTATTTTAGATAAA
    CAACGTGCTTTAAATGAAGCACAAGAAGTTACGAAATCATTGCCTATTAAAGACAACATC
    ATTACTGATAGTGGTGTCTATGTAGAAGGTTTAGAAAATGTACTTATCAAGTTGTCAAAA
    TGTTGTAATCCTATACCAGGTGATGATATTGTAGGTTATATCACCAAAGGTCACGGTATT
    AAAGTACATCGCACTGATTGCCCAAATATTAAGAACGAAACTGAACGACTAATTAATGTT
    GAATGGGTAAAATCAAAAGACGCAACTCAAAAATATCAGGTTGATTTAGAGGTAACTGCG
    TATGACCGAAATGGCTTGTTGAATGAAGTACTACAAGCTGTTAGCTCGACAGCCGGCAAT
    TTAATTAAAGTTTCAGGACGTTCAGATATTGATAAAAATGCAATAATAAATATTAGTGTC
    ATGGTGAAAAACGTGAATGATGTTTATCGTGTGGTAGAAAAGATCAAACAACTTGGTGAT
    GTTTATACAGTAACAAGAGTTTGGAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2190

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1689 [new locus tag: SACOL_RS08615 ]
  • symbol: RelA2
  • description: GTP pyrophosphokinase
  • length: 729
  • theoretical pI: 8.59224
  • theoretical MW: 83693.5
  • GRAVY: -0.438683

⊟Function[edit | edit source]

  • reaction:
    EC 2.7.6.5?  ExPASy
    GTP diphosphokinase ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate
  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions RelA/SpoT family protein (TIGR00691; HMM-score: 802.9)
    and 3 more
    TIGR04552 family protein (TIGR04552; HMM-score: 15.8)
    Unknown function General TIGR04562 family protein (TIGR04562; HMM-score: 14.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family (TIGR00042; EC 3.6.1.-; HMM-score: 13.1)
  • TheSEED  :
    • GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II
    • Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Stringent Response, (p)ppGpp metabolism  GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I
    and 1 more
    Regulation and Cell signaling Signal transduction in Eukaryotes Plastidial (p)ppGpp-mediated response in plants  GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I
  • PFAM:
    HD_PDEase (CL0237) HD_4; HD domain (PF13328; HMM-score: 160.3)
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 136.5)
    and 5 more
    Ubiquitin (CL0072) TGS; TGS domain (PF02824; HMM-score: 72.1)
    ACT (CL0070) ACT_4; ACT domain (PF13291; HMM-score: 67.3)
    HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 46.1)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 30.2)
    Ubiquitin (CL0072) Ub-Mut7C; Mut7-C ubiquitin (PF14451; HMM-score: 14.3)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005505
    • TAT(Tat/SPI): 0.000365
    • LIPO(Sec/SPII): 0.001436
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFRKNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIVDGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQIRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYIETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSINDCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHEIAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQSDKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIVEIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFRVEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNEAQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHRTDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLIKVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 80 [4]
  • interaction partners:
    SACOL0937(dltC)D-alanine--poly(phosphoribitol) ligase subunit 2  [5] (data from MRSA252)
    SACOL0593(fusA)elongation factor G  [5] (data from MRSA252)
    SACOL0033(mecA)penicillin-binding protein 2'  [5] (data from MRSA252)
    SACOL2220(rplO)50S ribosomal protein L15  [5] (data from MRSA252)
    SACOL2206(rpsI)30S ribosomal protein S9  [5] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 44.24 h [6]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  6. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

Tobias Geiger, Christiane Goerke, Michaela Fritz, Tina Schäfer, Knut Ohlsen, Manuel Liebeke, Michael Lalk, Christiane Wolz
Role of the (p)ppGpp synthase RSH, a RelA/SpoT homolog, in stringent response and virulence of Staphylococcus aureus.
Infect Immun: 2010, 78(5);1873-83
[PubMed:20212088] [WorldCat.org] [DOI] (I p)

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