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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1536 [new locus tag: NWMN_RS08620 ]
  • pan locus tag?: SAUPAN004239000
  • symbol: relA
  • pan gene symbol?: relA
  • synonym:
  • product: GTP pyrophosphokinase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1536 [new locus tag: NWMN_RS08620 ]
  • symbol: relA
  • product: GTP pyrophosphokinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1700948..1703158
  • length: 2211
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGAATGGGGTGTATCATATAATGAACAACGAATATCCATATAGTGCAGACGAAGTTCTT
    CACAAAGCAAAATCATATTTGTCAGCAGATGAATATGAGTATGTTTTAAAAAGCTATCAT
    ATTGCTTATGAAGCACATAAAGGTCAGTTCCGAAAAAACGGATTACCATACATTATGCAT
    CCTATACAAGTTGCAGGTATTTTAACAGAAATGCGATTAGACGGACCGACGATTGTCGCA
    GGTTTTTTGCATGATGTAATTGAAGATACACCGTATACATTTGAAGATGTAAAAGAAATG
    TTCAATGAAGAAGTTGCTCGAATTGTTGATGGTGTGACGAAGCTTAAAAAAGTAAAATAC
    CGCTCAAAAGAAGAACAACAAGCTGAAAATCATCGCAAGTTATTTATTGCGATTGCCAAA
    GATGTACGCGTAATTTTGGTGAAATTAGCAGACAGATTACATAATATGCGTACCTTGAAA
    GCCATGCCGCGCGAAAAACAAATTAGAATTTCTCGAGAAACATTAGAAATTTATGCACCA
    TTAGCACATCGTCTTGGTATTAATACAATCAAATGGGAACTAGAAGATACGGCTCTTCGT
    TATATTGATAATGTGCAATATTTTAGAATAGTCAATTTAATGAAGAAGAAACGTAGTGAA
    CGTGAAGCGTATATCGAAACGGCTATTGATAGAATACGTACTGAAATGGACCGAATGAAT
    ATCGAAGGCGATATAAATGGTAGACCTAAACATATTTACAGTATTTATCGGAAAATGATG
    AAGCAGAAAAAACAATTTGATCAAATTTTTGATTTGTTGGCGATACGTGTTATTGTCAAT
    TCTATTAATGATTGTTATGCGATACTTGGGTTGGTGCATACGTTATGGAAACCGATGCCA
    GGACGTTTTAAAGATTATATTGCAATGCCTAAACAAAATTTGTATCAGTCATTGCATACT
    ACAGTAGTAGGCCCAAATGGAGACCCGCTCGAAATCCAAATACGAACGTTTGATATGCAC
    GAAATTGCTGAGCATGGTGTTGCAGCACACTGGGCTTACAAAGAAGGTAAAAAAGTAAGT
    GAAAAAGATCAAACTTATCAAAATAAGTTAAATTGGTTAAAAGAATTAGCTGAAGCGGAT
    CATACATCGTCTGACGCTCAAGAATTTATGGAAACCTTAAAATATGACTTACAGAGTGAC
    AAAGTATACGCATTTACCCCAGCGAGTGATGTTATTGAGTTGCCATATGGTGCTGTGCCG
    ATTGATTTTGCTTATGCGATTCACAGTGAAGTAGGTAATAAGATGATTGGTGCCAAGGTG
    AATGGCAAAATTGTACCAATTGACTATATTTTACAAACAGGCGATATTGTTGAAATACGT
    ACTAGTAAACATTCATATGGACCAAGTCGTGATTGGTTGAAAATTGTTAAATCGTCTAGT
    GCCAAAGGTAAAATTAAAAGTTTCTTCAAAAAACAAGATCGTTCATCTAATATTGAAAAA
    GGCCGAATGATGGTTGAAGCTGAAATAAAAGAGCAAGGATTTAGAGTCGAAGATATTTTG
    ACAGAGAAAAATATTCAGGTTGTTAATGAAAAATATAACTTTGCAAATGAAGATGATTTA
    TTCGCAGCTGTAGGATTTGGCGGCGTGACATCCTTACAGATTGTTAATAAATTAACTGAA
    AGACAACGTATTTTAGATAAACAACGTGCTTTAAATGAAGCACAAGAAGTTACGAAATCA
    TTGCCTATTAAAGACAACATCATTACTGATAGTGGTGTCTATGTAGAAGGTTTAGAAAAT
    GTACTTATCAAGTTGTCAAAATGTTGTAATCCTATACCAGGTGATGATATTGTAGGTTAT
    ATCACCAAAGGTCACGGTATTAAAGTACATCGCACTGATTGCCCAAATATTAAGAACGAA
    ACTGAACGACTAATTAATGTTGAATGGGTAAAATCAAAAGACGCAACTCAAAAATATCAG
    GTTGATTTAGAGGTAACTGCGTATGACCGAAATGGCTTGTTGAATGAAGTACTACAAGCT
    GTTAGCTCGACAGCCGGCAATTTAATTAAAGTTTCAGGACGTTCAGATATTGATAAAAAT
    GCAATAATAAATATTAGTGTCATGGTGAAAAACGTGAATGATGTTTATCGTGTGGTAGAA
    AAGATCAAACAACTTGGTGATGTTTATACAGTAACAAGAGTTTGGAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2211

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1536 [new locus tag: NWMN_RS08620 ]
  • symbol: RelA
  • description: GTP pyrophosphokinase
  • length: 736
  • theoretical pI: 8.58887
  • theoretical MW: 84508.5
  • GRAVY: -0.431522

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Adaptations to atypical conditions RelA/SpoT family protein (TIGR00691; HMM-score: 802.8)
    and 3 more
    TIGR04552 family protein (TIGR04552; HMM-score: 15.8)
    Unknown function General TIGR04562 family protein (TIGR04562; HMM-score: 14.9)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair non-canonical purine NTP pyrophosphatase, RdgB/HAM1 family (TIGR00042; EC 3.6.1.-; HMM-score: 13.1)
  • TheSEED  :
    • GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II
    • Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)
    Regulation and Cell signaling Regulation and Cell signaling - no subcategory Stringent Response, (p)ppGpp metabolism  GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I
    and 1 more
    Regulation and Cell signaling Signal transduction in Eukaryotes Plastidial (p)ppGpp-mediated response in plants  GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I
  • PFAM:
    HD_PDEase (CL0237) HD_4; HD domain (PF13328; HMM-score: 160.4)
    NTP_transf (CL0260) RelA_SpoT; Region found in RelA / SpoT proteins (PF04607; HMM-score: 156)
    and 7 more
    Ubiquitin (CL0072) TGS; TGS domain (PF02824; HMM-score: 77.4)
    ACT (CL0070) ACT_4; ACT domain (PF13291; HMM-score: 63.2)
    HD_PDEase (CL0237) HD; HD domain (PF01966; HMM-score: 54.4)
    no clan defined RelA_AH_RIS; RelA/SpoT, AH and RIS domains (PF19296; HMM-score: 36.1)
    ACT (CL0070) ACT; ACT domain (PF01842; HMM-score: 32.3)
    Ubiquitin (CL0072) Ub-Mut7C; Mut7-C ubiquitin (PF14451; HMM-score: 13.8)
    no clan defined DUF5890; Family of unknown function (DUF5890) (PF19240; HMM-score: 10.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7968
    • Cytoplasmic Membrane Score: 0.0269
    • Cell wall & surface Score: 0.0004
    • Extracellular Score: 0.1759
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.007678
    • TAT(Tat/SPI): 0.000263
    • LIPO(Sec/SPII): 0.001032
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNGVYHIMNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFRKNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIVDGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQIRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYIETAIDRIRTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSINDCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHEIAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQSDKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIVEIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFRVEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNEAQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHRTDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLIKVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_0805(dltC)D-alanine--poly(phosphoribitol) ligase subunit 2  [1] (data from MRSA252)
    NWMN_0509(fus)elongation factor G  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_2119(rpsI)30S ribosomal protein S9  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 1.2 1.3 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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