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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1687 [new locus tag: SACOL_RS08605 ]
  • pan locus tag?: SAUPAN004237000
  • symbol: SACOL1687
  • pan gene symbol?: lytH
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1687 [new locus tag: SACOL_RS08605 ]
  • symbol: SACOL1687
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: -
  • coordinates: 1716773..1717648
  • length: 876
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGAAAAAAATAGAGGCATGGTTATCTAAAAAGGGTCTTAAAAATAAACGTACTCTAATA
    GTAGTGATTGCCTTTGTCTTATTTATCATCTTTTTATTTTTATTGCTGAATAGCAATAGT
    GAAGATAGTGGGAACATCACGATAACTGAAAATGCTGAATTACGTACAGGTCCAAACGCT
    GCGTATCCAGTCATATATAAAGTTGAAAAAGGTGACCATTTTAAAAAGATTGGTAAAGTA
    GGTAAATGGATTGAAGTTGAAGATACATCCAGTAATGAAAAAGGTTGGATAGCTGGATGG
    CACACAAATTTAGATATTGTCGCGGATAATACGAAGGAGAAAAATCCTTTGCAAGGTAAA
    ACAATAGTGCTTGATCCTGGTCATGGAGGTAGTGACCAGGGTGCTTCAAGCAATACTAAA
    TATAAAAGTTTAGAAAAAGATTATACGTTGAAAACAGCAAAAGAATTGCAGCGTACTTTA
    GAAAAAGAAGGCGCAACTGTTAAGATGACAAGAACAGACGATACATATGTTTCACTAGAA
    AATCGTGATATCAAAGGCGATGCCTATTTGAGTATACATAATGATGCGTTAGAATCATCT
    AATGCAAATGGAATGACAGTTTATTGGTATCATGATAATCAAAGAGCTTTAGCAGATACG
    TTAGACGCTACGATTCAGAAGAAAGGTCTACTTTCTAATCGCGGTTCAAGACAAGAAAAT
    TATCAAGTGTTAAGACAAACAAAAGTTCCTGCTGTTTTATTAGAATTAGGTTATATTAGT
    AACCCAACTGATGAAACGATGATTAAAGATCAATTACATAGACAAATTTTAGAACAAGCA
    ATTGTTGATGGCCTTAAAATTTATTTTTCTGCGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    876

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1687 [new locus tag: SACOL_RS08605 ]
  • symbol: SACOL1687
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 291
  • theoretical pI: 8.61491
  • theoretical MW: 32693.9
  • GRAVY: -0.505842

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.1.-?  ExPASy
  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 115.1)
    Cellular processes Cellular processes Sporulation and germination N-acetylmuramoyl-L-alanine amidase CwlD (TIGR02883; EC 3.5.1.28; HMM-score: 115.1)
    and 1 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking preprotein translocase, SecE subunit (TIGR00964; HMM-score: 14.7)
  • TheSEED  :
    • LytH protein involved in methicillin resistance
    • N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) domain
  • PFAM:
    Peptidase_MH (CL0035) Amidase_3; N-acetylmuramoyl-L-alanine amidase (PF01520; HMM-score: 152.3)
    and 5 more
    SH3 (CL0010) SH3_3; Bacterial SH3 domain (PF08239; HMM-score: 31)
    SH3_4; Bacterial SH3 domain (PF06347; HMM-score: 20)
    no clan defined DUF4969; Domain of unknown function (DUF4969) (PF16339; HMM-score: 13.3)
    O-anti_assembly (CL0499) O-antigen_lig; O-antigen ligase like membrane protein (PF13425; HMM-score: 8.4)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 7.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 8.28
    • Cellwall Score: 1.71
    • Extracellular Score: 0.01
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.408033
    • TAT(Tat/SPI): 0.00118
    • LIPO(Sec/SPII): 0.083029
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSGNITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHTNLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTLEKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALADTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQILEQAIVDGLKIYFSA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Annette Dreisbach, Kristina Hempel, Girbe Buist, Michael Hecker, Dörte Becher, Jan Maarten van Dijl
    Profiling the surfacome of Staphylococcus aureus.
    Proteomics: 2010, 10(17);3082-96
    [PubMed:20662103] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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