PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL1646 [new locus tag: SACOL_RS08395 ]
• pan locus tag^?: SAUPAN004176000
• symbol: comEA
• pan gene symbol^?: comEA
• synonym:
• product: comE operon protein 1-like protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL1646 [new locus tag: SACOL_RS08395 ]
• symbol: comEA
• product: comE operon protein 1-like protein
• replicon: chromosome
• strand: -
• coordinates: 1678152..1678838
• length: 687
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3238458 NCBI
• RefSeq: YP_186486 NCBI
• BioCyc: see SACOL_RS08395
• MicrobesOnline: 913095 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTA
  TATATTCTAAGTGCTGTTGTTTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAA
  GATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTGCTCTGAAACAAAGCACTAGT
  GAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
  AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAA
  ATGACATCTAAGGATAGAGTAGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCA
  GATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATCAAAAAATGATTTTCATACCT
  CATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
  GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCT
  GTTCCTGGAGTTGGGCAAGCTAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGT
  GCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTTTTGGAAGTAAAACTTTTGAT
  AAACTGAAATCTTATTTCACGACATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  687
  

⊟Protein

⊟General

• locus tag: SACOL1646 [new locus tag: SACOL_RS08395 ]
• symbol: ComEA
• description: comE operon protein 1-like protein
• length: 228
• theoretical pI: 9.36125
• theoretical MW: 25863.3
• GRAVY: -0.462719

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 116.3)
  and 4 more
  Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 74.2)
  Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 20.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.3)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.4)
• TheSEED  :
  • Late competence protein ComEA, DNA receptor
  DNA Metabolism DNA uptake, competence Gram Positive Competence  Late competence protein ComEA, DNA receptor
  and 1 more
  DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEA, DNA receptor
• PFAM:
  HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.7)
  and 11 more
  HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 46.1)
  HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 29.3)
  Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 26.9)
  HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 22.4)
  HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 19.8)
  T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 18.3)
  HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 16)
  RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 14.9)
  PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 14.8)
  O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 14.6)
  Aha1_BPI (CL0648) DUF1439; Protein of unknown function (DUF1439) (PF07273; HMM-score: 13.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001648
  • TAT(Tat/SPI): 0.000233
  • LIPO(Sec/SPII): 0.001342
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 57651982 NCBI
• RefSeq: YP_186486 NCBI
• UniProt: A0A0H2WZ91 UniProt

⊟Protein sequence

• MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: comEA < SACOL1647 < SACOL1648 < SACOL1649 < nadD < SACOL1651 < aroE < SACOL1653 < SACOL1654 < mtn

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications