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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1653 [new locus tag: SACOL_RS08430 ]
  • pan locus tag?: SAUPAN004183000
  • symbol: SACOL1653
  • pan gene symbol?: —
  • synonym:
  • product: GTP-binding protein YqeH

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1653 [new locus tag: SACOL_RS08430 ]
  • symbol: SACOL1653
  • product: GTP-binding protein YqeH
  • replicon: chromosome
  • strand: -
  • coordinates: 1682211..1683311
  • length: 1101
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGTCTGACATTTTAAAATGTATCGGTTGTGGTGCGCCACTTCAATCTGAAGATAAAAAT
    AAACCTGGTTTTGTACCAGAGCATAATATGTTTCGTGATGACGTGATTTGCAGACGTTGT
    TTCCGCTTGAAAAATTATAACGAAGTTCAAGATGTAGGATTAGAAAGTGAAGACTTTTTA
    AAATTATTATCAGGACTTGCGGATAAAAAGGGTATTGTCGTCAATGTCGTGGATGTATTT
    GACTTTGAAGGATCATTTATTAATGCAGTTAAACGTATTGTCGGAAACAAAAAAATCATT
    TTAGCAGCAAATAAATTGGATTTATTGCCGAAACAAATTAATAAACGTCGAGTTAAAGAA
    TGGTTAAAACGAACAGCAAGAAAATATGGTTTGGAAGCTGACGATGTCGTATTAATTTCA
    GCTGAAAAAGGCTGGGGCATAGACGACTTATTATCATCAATTGCGAATATTCGAGAAAAT
    GAAGATGTGTATATTGTAGGGACAACGAATGTTGGGAAATCTACATTGATTAATAAACTG
    ATTGAAGCTAGTGTTGGTGAAAAAGATGTAGTAACAACTTCAAGATTCCCTGGAACAACT
    TTAGATATGATAGATATTCCTTTAGATGAAACATCATTTATGTATGATACACCAGGTATT
    ATTCAAGATCACCAAATGACGCATTTAGTTAGTGAAAAAGAATTGAAAATTATTATGCCT
    AAGAAAGAAATAAAACAACGCGTATATCAATTAAATGAGGCGCAGACATTATTCTTCGGC
    GGTCTAGCGCGCATAGATTATGTATCAGGTGGTAAACGTCCGTTAGTTTGTTTCTTTTCT
    AATGACTTGAATATACATCGTACTAAAACGGAGAAGGCTAATGATTTATGGCGTAATCAA
    CTTGGCGATTTATTAACGCCACCTGGAAATCCACAAAATTTTGATCTTAATGAGGTAAAG
    GCTGTTAGACTTGAAACAGGCAAAGAGAAACGCGATGTTATGATCTCTGGTCTAGGCTTT
    ATAACTATAGGACCAGGGGCTAAAGTAATCGTTCGTGTTCCTAAAAATGTTGATGTTGTA
    TTAAGAAATTCTATTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1101

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1653 [new locus tag: SACOL_RS08430 ]
  • symbol: SACOL1653
  • description: GTP-binding protein YqeH
  • length: 366
  • theoretical pI: 8.44888
  • theoretical MW: 41086.3
  • GRAVY: -0.22459

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein synthesis Other ribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 486.9)
    and 19 more
    Genetic information processing Protein synthesis Other ribosome-associated GTPase EngA (TIGR03594; HMM-score: 60.6)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 52.5)
    Genetic information processing Protein synthesis Other ribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 40)
    Unknown function General GTP-binding protein HflX (TIGR03156; HMM-score: 37.5)
    Unknown function General small GTP-binding protein domain (TIGR00231; HMM-score: 37.4)
    Genetic information processing Protein synthesis Other GTP-binding protein Era (TIGR00436; HMM-score: 33.2)
    Genetic information processing Protein synthesis Other Obg family GTPase CgtA (TIGR02729; HMM-score: 32.9)
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 30.5)
    Genetic information processing Protein synthesis Translation factors ribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 28.5)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 28.2)
    Metabolism Transport and binding proteins Cations and iron carrying compounds ferrous iron transport protein B (TIGR00437; HMM-score: 22.9)
    Genetic information processing Protein synthesis Translation factors selenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 21.9)
    Unknown function General elongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 18.5)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 18.4)
    Metabolism Energy metabolism Amino acids and amines ethanolamine utilization protein, EutP (TIGR02528; HMM-score: 15.3)
    Genetic information processing Protein synthesis Translation factors translation initiation factor IF-2 (TIGR00487; HMM-score: 14.2)
    Unknown function General GTP-binding protein YchF (TIGR00092; HMM-score: 13.9)
    Metabolism Transport and binding proteins Unknown substrate energy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 11.7)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCb (TIGR03925; HMM-score: 11)
  • TheSEED  :
    • GTP-binding protein YqeH, required for biogenesis of 30S ribosome subunit
    Protein Metabolism Protein biosynthesis Universal GTPases  GTP-binding protein YqeH, required for biogenesis of 30S ribosome subunit
  • PFAM:
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 54.2)
    and 18 more
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 39.8)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 34.6)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 27.3)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 21.9)
    Ras; Ras family (PF00071; HMM-score: 19.6)
    Dynamin_N; Dynamin family (PF00350; HMM-score: 18.8)
    ABC_tran; ABC transporter (PF00005; HMM-score: 17.2)
    no clan defined MMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 17)
    P-loop_NTPase (CL0023) ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.3)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 14.4)
    ArgK; ArgK protein (PF03308; HMM-score: 13.4)
    AAA_18; AAA domain (PF13238; HMM-score: 12.8)
    PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 12.4)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.3)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 12.2)
    no clan defined Swi5; Swi5 (PF07061; HMM-score: 12.2)
    P-loop_NTPase (CL0023) FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 12.1)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 8.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005254
    • TAT(Tat/SPI): 0.000227
    • LIPO(Sec/SPII): 0.000589
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLKNYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILAANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIRENEDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTPGIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLVCFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVMISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 86 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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