PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1492 [new locus tag: NWMN_RS08400 ]
• pan locus tag^?: SAUPAN004176000
• symbol: comEA
• pan gene symbol^?: comEA
• synonym:
• product: competence protein ComEA

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1492 [new locus tag: NWMN_RS08400 ]
• symbol: comEA
• product: competence protein ComEA
• replicon: chromosome
• strand: -
• coordinates: 1660991..1661668
• length: 678
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331834 NCBI
• RefSeq: YP_001332526 NCBI
• BioCyc:
• MicrobesOnline: 3707044 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTA
  AGTGCTGTTGTTTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTAT
  ACTTCAAGAAATTTTGAAAATAAAGATACTGCTCTGAAACAAAGCACTAGTGAAAATAGT
  AGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAAAATAAGGGT
  CCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCT
  AAGGATAGAGTAGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGT
  CAAATTAATTTGTCTGAAAAATTAACAGATCAAAAAATGATTTTCATACCTCATAAAGGA
  CAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAATGGGAACACA
  AATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGA
  GTTGGGCAAGCTAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAA
  GAAATTGACGATTTGAAAAAAGTAAAAGGTTTTGGAAGTAAAACTTTTGATAAACTGAAA
  TCTTATTTCACGACATAA
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⊟Protein

⊟General

• locus tag: NWMN_1492 [new locus tag: NWMN_RS08400 ]
• symbol: ComEA
• description: competence protein ComEA
• length: 225
• theoretical pI: 9.36125
• theoretical MW: 25537.9
• GRAVY: -0.521333

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 116.3)
  and 4 more
  Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 74.2)
  Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 20.7)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.3)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.7)
  and 11 more
  HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 46.2)
  HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 29.4)
  Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 26.9)
  HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 22.4)
  HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 19.9)
  T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 18.4)
  HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 16)
  RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 15)
  PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 14.8)
  Aha1_BPI (CL0648) DUF1439; Protein of unknown function (DUF1439) (PF07273; HMM-score: 13.8)
  O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helix: 1
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 4
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004614
  • TAT(Tat/SPI): 0.000377
  • LIPO(Sec/SPII): 0.002017
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI: 151221704 NCBI
• RefSeq: YP_001332526 NCBI
• UniProt: A0A0H3K8T8 UniProt

⊟Protein sequence

• MYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: holA < comEC < comEB < comEA < NWMN_1493 < NWMN_1494 < NWMN_1495 < NWMN_1496 < NWMN_1497 < aroE < NWMN_1499 < NWMN_1500 < pfs

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications