PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1491 [new locus tag: NWMN_RS08395 ]
• pan locus tag^?: SAUPAN004175000
• symbol: comEB
• pan gene symbol^?: comEB
• synonym:
• product: competence protein ComEB required for DNA binding and uptake

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1491 [new locus tag: NWMN_RS08395 ]
• symbol: comEB
• product: competence protein ComEB required for DNA binding and uptake
• replicon: chromosome
• strand: -
• coordinates: 1660438..1660899
• length: 462
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5331835 NCBI
• RefSeq: YP_001332525 NCBI
• BioCyc:
• MicrobesOnline: 3707043 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  
  TTGGAAAGAATCAAATGGGAAGAATATTTTATGGCACAAAGTCATTTGCTAGCATTACGT
  TCAACTTGTCAAAGATTATCTGTAGGTGCAACGATTGTTAAGGATAATCGTATTATTGCT
  GGTGGTTATAATGGCTCTGTAGCTGGCGAGGTGCATTGTATAGATGAAGGATGTTTAATT
  GAAGATGGACATTGTATCAGAACGATACATGCAGAAATGAATGCTTTATTACAATGTGCA
  AAACAAGGTGTATCTACTGAAGGTGCAACAATCTATGTTACTCATTTTCCATGCCTAAAT
  TGTACAAAGTCAATTATTCAAGCAGGTATAAAGCGTATCTACTATGCAGAAGATTATCAT
  AACCATGAATATGCAACTAAATTACTCAAACAATCTGGTATTGAATTTAAAAAAATTCCA
  TTTTCACCAGAATATGTTGCTAAATATCTGACTAAAGGTTAA
  60
  120
  180
  240
  300
  360
  420
  462
  

⊟Protein

⊟General

• locus tag: NWMN_1491 [new locus tag: NWMN_RS08395 ]
• symbol: ComEB
• description: competence protein ComEB required for DNA binding and uptake
• length: 153
• theoretical pI: 7.79403
• theoretical MW: 17175.7
• GRAVY: -0.164706

⊟Function

• TIGRFAM:
  ComE operon protein 2 (TIGR02571; HMM-score: 292.6)
  and 1 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin biosynthesis protein RibD (TIGR00326; EC 1.1.1.193,3.5.4.26; HMM-score: 38.1)
• TheSEED  :
  • dCMP deaminase (EC 3.5.4.12)
  • Late competence protein ComEB
  DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEB
• PFAM:
  CDA (CL0109) dCMP_cyt_deam_1; Cytidine and deoxycytidylate deaminase zinc-binding region (PF00383; HMM-score: 99.5)
  and 11 more
  MafB19-deam; MafB19-like deaminase (PF14437; HMM-score: 71.7)
  Bd3614-deam; Bd3614-like deaminase (PF14439; HMM-score: 23.4)
  SNAD4; Secreted Novel AID/APOBEC-like Deaminase 4 (PF18750; HMM-score: 18.6)
  APOBEC2; APOBEC2 (PF18772; HMM-score: 17.8)
  APOBEC_N; APOBEC-like N-terminal domain (PF08210; HMM-score: 16.3)
  APOBEC4_like; APOBEC4-like -AID/APOBEC-deaminase (PF18774; HMM-score: 14.8)
  NAD2; Novel AID APOBEC clade 2 (PF18782; HMM-score: 14.5)
  NAD1; Novel AID APOBEC clade 1 (PF18778; HMM-score: 13.9)
  OTT_1508_deam; OTT_1508-like deaminase (PF14441; HMM-score: 13.3)
  APOBEC3; APOBEC3 (PF18771; HMM-score: 13.1)
  no clan defined DUF3880; DUF based on E. rectale Gene description (DUF3880) (PF12996; HMM-score: 13)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9605
  • Cytoplasmic Membrane Score: 0.0015
  • Cell wall & surface Score: 0.0002
  • Extracellular Score: 0.0378
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.090128
  • TAT(Tat/SPI): 0.00771
  • LIPO(Sec/SPII): 0.024472
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221703 NCBI
• RefSeq: YP_001332525 NCBI
• UniProt: A0A0H3K8F5 UniProt

⊟Protein sequence

• MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYNGSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCTKSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: holA < comEC < comEB < comEA < NWMN_1493 < NWMN_1494 < NWMN_1495 < NWMN_1496 < NWMN_1497 < aroE < NWMN_1499 < NWMN_1500 < pfs

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications