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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1417 [new locus tag: SA_RS08000 ]
  • pan locus tag?: SAUPAN004175000
  • symbol: comEB
  • pan gene symbol?: comEB
  • synonym:
  • product: late competence operon required for DNA binding and uptake comEB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1417 [new locus tag: SA_RS08000 ]
  • symbol: comEB
  • product: late competence operon required for DNA binding and uptake comEB
  • replicon: chromosome
  • strand: -
  • coordinates: 1624543..1625004
  • length: 462
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGGAAAGAATCAAATGGGAAGAATATTTTATGGCACAAAGTCATTTGCTAGCATTACGT
    TCAACTTGTCAAAGATTATCTGTAGGTGCAACGATTGTTAAGGATAATCGTATTATTGCT
    GGTGGTTATAATGGCTCTGTAGCTGGCGAGGTGCATTGTATAGATGAAGGATGTTTAATT
    GAAGATGGACATTGTATCAGAACGATACATGCAGAAATGAATGCTTTATTACAATGTGCA
    AAACAAGGTGTATCTACTGAAGGTGCAACAATCTATGTTACTCATTTTCCATGCCTAAAT
    TGTACAAAGTCAATTATTCAAGCAGGTATAAAGCGTATCTACTATGCAGAAGATTATCAT
    AACCATGAATATGCAACTAAATTACTCAAACAATCTGGTATTGAATTTAAAAAAATTCCA
    TTTTCACCAGAATATGTTGCTAAATATCTGACTAAAGGTTAA
    60
    120
    180
    240
    300
    360
    420
    462

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1417 [new locus tag: SA_RS08000 ]
  • symbol: ComEB
  • description: late competence operon required for DNA binding and uptake comEB
  • length: 153
  • theoretical pI: 7.79403
  • theoretical MW: 17175.7
  • GRAVY: -0.164706

⊟Function[edit | edit source]

  • TIGRFAM:
    ComE operon protein 2 (TIGR02571; HMM-score: 292.6)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Riboflavin, FMN, and FAD riboflavin biosynthesis protein RibD (TIGR00326; EC 1.1.1.193,3.5.4.26; HMM-score: 38.1)
  • TheSEED  :
    • dCMP deaminase (EC 3.5.4.12)
    • Late competence protein ComEB
    DNA Metabolism DNA uptake, competence Late competence  Late competence protein ComEB
  • PFAM:
    CDA (CL0109) dCMP_cyt_deam_1; Cytidine and deoxycytidylate deaminase zinc-binding region (PF00383; HMM-score: 106)
    and 2 more
    MafB19-deam; MafB19-like deaminase (PF14437; HMM-score: 70.6)
    Bd3614-deam; Bd3614-like deaminase (PF14439; HMM-score: 14.5)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.090128
    • TAT(Tat/SPI): 0.00771
    • LIPO(Sec/SPII): 0.024472
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYNGSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCTKSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SigH* regulon
    SigH*(sigma factor)controlling competence and phage integrase genes;  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kazuya Morikawa, Yumiko Inose, Hideyuki Okamura, Atsushi Maruyama, Hideo Hayashi, Kunio Takeyasu, Toshiko Ohta
    A new staphylococcal sigma factor in the conserved gene cassette: functional significance and implication for the evolutionary processes.
    Genes Cells: 2003, 8(8);699-712
    [PubMed:12875655] [WorldCat.org] [DOI] (P p)

⊟Relevant publications[edit | edit source]

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