PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1415 [new locus tag: SA_RS07990 ]
• pan locus tag^?: SAUPAN004173000
• symbol: holA
• pan gene symbol^?: holA
• synonym:
• product: DNA polymerase III subunit delta

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1415 [new locus tag: SA_RS07990 ]
• symbol: holA
• product: DNA polymerase III subunit delta
• replicon: chromosome
• strand: -
• coordinates: 1621306..1622280
• length: 975
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124257 NCBI
• RefSeq: NP_374700 NCBI
• BioCyc: see SA_RS07990
• MicrobesOnline: 103726 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAGCGACAATATTGTAGCTATTTATGGAGATGTGCCTGAATTGGTTGAAAAACAAAGT
  GCAGAAATCATATCACAATTTTTGAAAAGTGATAGAGATGACTTTAACTTTGTGAAATAT
  AATTTATACGAAAAAGAGATTGCACCAATTGTTGAAGAAACATTAACATTGCCTTTCTTT
  TCAGATAAAAAAGCAATTTTGGTTAAAAATGCATATATATTTACAGGTGAAAAAGCGCCA
  AAAGATATGGCTCATAATGTAGATCAATTAATAGAATTTATTGAAAAATATGATGGCGAA
  AATTTGATTGTCTTTGAGATATATCAAAATAAACTTGATGAAAGAAAAAAGTTAACTAAA
  ACTCTAAAAAAGCATGCAAGGCTTAAAAAAATAGAGCAAATGTCTGAAGAAGAAATAAAA
  AAATGGATTCAAAGTAAATTAAATGAGAATTTCAAAGATATCAAAAGAGATGCATTAGAT
  TTATTTATTGAGTTGACAGGTATTAACTTTAATATTGTCTCACAAGAGATAGAAAAGTTG
  ATTTTATTTTTAGGCGATAGACCAACAATTAATAAGCAGGATGTTAACCAAATTATTAAT
  AGAAGTTTAGAGCAAAATGTATTTTTACTGACTGAATACATTCAGAAAAGAAAGAAAGAA
  CAAGCAATTCATTTAGTAAAAGATTTAATAACTATGAAAGAAGAACCAATTAAATTACTT
  GCACTAATTACAAGTAATTACCGATTATTTTATCAATGTAAGATTCTGAGTCAAAAAGGA
  TATAGTGGACAGCAAATTGCTAAAACAATAGGAGTGCATCCATACAGAGTAAAATTAGCG
  TTAGGACAAGTAAGACATTATCAACTTGATGAATTATTGAATATTGTAGATGCTTGTGCG
  GAAACTGATTATAAACTTAAATCATCATATATGGATAAACAGTTAATACTGGAATTATTC
  ATTCTATCTTTATAA
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⊟Protein

⊟General

• locus tag: SA1415 [new locus tag: SA_RS07990 ]
• symbol: HolA
• description: DNA polymerase III subunit delta
• length: 324
• theoretical pI: 7.06335
• theoretical MW: 37948.8
• GRAVY: -0.311111

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, delta subunit (TIGR01128; EC 2.7.7.7; HMM-score: 296.2)
  and 1 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA polymerase III, subunit gamma and tau (TIGR02397; EC 2.7.7.7; HMM-score: 14.9)
• TheSEED  :
  • DNA polymerase III delta subunit, clamp loader part A
  DNA Metabolism DNA replication DNA-replication  DNA polymerase III delta subunit (EC 2.7.7.7)
• PFAM:
  P-loop_NTPase (CL0023) DNA_pol3_delta; DNA polymerase III, delta subunit (PF06144; HMM-score: 170.2)
  and 7 more
  no clan defined DUF2018; Domain of unknown function (DUF2018) (PF09442; HMM-score: 17.2)
  DUF3781; Protein of unknown function (DUF3781) (PF12636; HMM-score: 15.6)
  DisA-linker; DisA bacterial checkpoint controller linker region (PF10635; HMM-score: 14.5)
  HTH (CL0123) HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 12.3)
  no clan defined DUF3819; Domain of unknown function (DUF3819) (PF12842; HMM-score: 11.9)
  P-loop_NTPase (CL0023) Sigma54_activ_2; Sigma-54 interaction domain (PF14532; HMM-score: 9.3)
  no clan defined DUF4163; Domain of unknown function (DUF4163) (PF13739; HMM-score: 8.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.001644
  • TAT(Tat/SPI): 0.000165
  • LIPO(Sec/SPII): 0.0003
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927167 NCBI
• RefSeq: NP_374700 NCBI
• UniProt: A0A0H3JQ91 UniProt

⊟Protein sequence

• MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYEKEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENLIVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALDLFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRKKEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYRVKLALGQVRHYQLDELLNIVDACAETDYKLKSSYMDKQLILELFILSL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: holA < SA1416 < comEB < SA1418

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications