PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1490 [new locus tag: NWMN_RS08390 ]
• pan locus tag^?: SAUPAN004174000
• symbol: comEC
• pan gene symbol^?: comEC
• synonym:
• product: DNA internalization-related competence protein ComEC/Rec2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1490 [new locus tag: NWMN_RS08390 ]
• symbol: comEC
• product: DNA internalization-related competence protein ComEC/Rec2
• replicon: chromosome
• strand: -
• coordinates: 1658232..1660433
• length: 2202
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332011 NCBI
• RefSeq: YP_001332524 NCBI
• BioCyc:
• MicrobesOnline: 3707042 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  TTGCTGTTTGTCGCGTTATCAATGATTGTAGGAGTGCTTTGGAATTCTAGCAAAGTGCTC
  TCTACATTTCTTTTCATTTTACTTTTGTATATTACTTATCGTAAAAATAAAATCGTTTAT
  GCCCCTATTTCTCTCTTTTTAATCATTTTCTCCGCATGGTATTTACATTATTCACAACAA
  GCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGTGCTCAA
  GTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTAAAAAAT
  GAAATATATCCTTTCTTTTTAACAGATAAAAAGAATTTTGATTTAAAGAAAATTGAAAGT
  CGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGACAATAAGTTTGTAACTCTTAAA
  TTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATTGAGAAA
  CATAAACAGTTTATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGATCGTATT
  ATGGCATTGATTACTGGTGACGTAAAAGAAGTTAATGAGCAATTTAAGGAACGTGTTAAA
  GAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGCATATAGCTGCAATTGTATTT
  TTAATTTACCAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGAATTACA
  ATCATTGTATTAGCTTTATTTGCTCAATACACAAATTATGCACCTAGTGCTGTAAGAGCT
  ATAATAATGACAACTCTTGTACTGGTTATTACTAAGCAAATTAAAATAAAGAGTATTCAG
  CTATTAGCATTTGCATTTATAATTATGTTTATTTTAAATCCACTAGTTGTTTATGATATT
  GGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTTTTACAG
  CAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCTTCATTT
  ATCGTTGCCATTCCAAGCTTTCATCAACTTCAATGGGTGGGATTTTTATCTAATTTGATT
  TTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTATTTTATTCTTTATTACAAGT
  CATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAATTTTCAT
  GACTGGTTACTAGACCTATTCACAAGAATCAAGCAATCACATTTTTCTGTTCCCAAGTTT
  AATGATTGGATATTTATAGTATTTATAATTTCTGTTTATTACATATTTTGGTTATTGGCT
  AAACGTAAATATATATTGGTTACGTTTTGGACTATAATTATTCTGACATTATTAATAACG
  TTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGACAGTATT
  TTATATGAAGGCGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTGATTGAT
  GATACTAAACAACCTAGTTATTCAATTTCTAAATATCATATTTTACCAACGCTAAATGAA
  AGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATCCACACAATGACCATATTGGT
  GAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGTGATATACAATAAGGGA
  TATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATTAAACTT
  ATGGATGTAAGACAAGTTAGTAGTTTTAAACTTGGAGATAGTAGTTTTCTATTTTTTGAT
  AGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGATTATTACTATGATTACATAT
  CAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTACTACTA
  AAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGACATCATGGGAGCAAGACA
  AGTAGTTCTAAAGAATTTATAGAGATGATTAAGCCTAAAATAAGTTTGATTTCTTCTGGG
  AAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTAAACGATTGCAAAGGATTCGC
  AGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGATAATTTA
  AAAGTTGATTCAAGCTCTTATGGAAATGCAAGTGGTTTATAG
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⊟Protein

⊟General

• locus tag: NWMN_1490 [new locus tag: NWMN_RS08390 ]
• symbol: ComEC
• description: DNA internalization-related competence protein ComEC/Rec2
• length: 733
• theoretical pI: 10.0286
• theoretical MW: 84839.2
• GRAVY: 0.263711

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes DNA transformation DNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM-score: 276.1)
  and 3 more
  Unknown function General ComEC/Rec2-related protein (TIGR00360; HMM-score: 75.8)
  Cellular processes Cellular processes Detoxification hydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 17.4)
  arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 15.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined Competence; Competence protein (PF03772; HMM-score: 178.4)
  and 3 more
  Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 80.3)
  Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 13.8)
  no clan defined DUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 11
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002313
  • TAT(Tat/SPI): 0.000575
  • LIPO(Sec/SPII): 0.045515
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: 151221702 NCBI
• RefSeq: YP_001332524 NCBI
• UniProt: A0A0H3KGS0 UniProt

⊟Protein sequence

• MLFVALSMIVGVLWNSSKVLSTFLFILLLYITYRKNKIVYAPISLFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEIYPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEKHKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKIKSIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFIAQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL

⊟Experimental data

• experimentally validated:
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: holA < comEC < comEB < comEA < NWMN_1493 < NWMN_1494 < NWMN_1495 < NWMN_1496 < NWMN_1497 < aroE < NWMN_1499 < NWMN_1500 < pfs

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications