PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

FunGene: 08-OCT-2024

⊟Summary

• organism: Staphylococcus aureus JSNZ
• locus tag: JSNZ_001580
• pan locus tag^?: SAUPAN004176000
• symbol: JSNZ_001580
• pan gene symbol^?: comEA
• synonym:
• product: helix-hairpin-helix domain-containing protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: JSNZ_001580
• symbol: JSNZ_001580
• product: helix-hairpin-helix domain-containing protein
• replicon: chromosome
• strand: -
• coordinates: 1618008..1618694
• length: 687
• essential: unknown other strains

⊟Accession numbers

• Gene ID:
• RefSeq:
• BioCyc:
• MicrobesOnline:

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  
  GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTA
  TATATTATAAGTGCTGTTGTTTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAA
  GATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTGCTCTGAAACAAAGCACTAGT
  GAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
  AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAA
  ATGACATCTAAGGATAGAGTAGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCA
  GATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATCAAAAAATGATTTTCATACCT
  CATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
  GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCT
  GTTCCTGGAGTAGGGCAAGCTAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGT
  GCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTTTTGGAAGTAAAACTTTTGAT
  AAACTGAAATCTTATTTCACGATATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  687
  

⊟Protein

⊟General

• locus tag: JSNZ_001580
• symbol: JSNZ_001580
• description: helix-hairpin-helix domain-containing protein
• length: 228
• theoretical pI: 9.68439
• theoretical MW: 25773.3
• GRAVY: -0.389474

⊟Function

• TIGRFAM:
  Cellular processes Cellular processes DNA transformation comEA protein (TIGR01259; HMM-score: 117.4)
  and 4 more
  Cellular processes Cellular processes DNA transformation competence protein ComEA helix-hairpin-helix repeat region (TIGR00426; HMM-score: 74.6)
  Unknown function Enzymes of unknown specificity putative DNA modification/repair radical SAM protein (TIGR03916; HMM-score: 20.6)
  Genetic information processing DNA metabolism DNA replication, recombination, and repair Holliday junction DNA helicase RuvA (TIGR00084; EC 3.6.4.12; HMM-score: 13.2)
  Metabolism Transport and binding proteins Nucleosides, purines and pyrimidines nucleoside Transporter (TIGR00939; HMM-score: 12.6)
• TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
• PFAM:
  HHH (CL0198) HHH_3; Helix-hairpin-helix motif (PF12836; HMM-score: 89.5)
  and 14 more
  HHH; Helix-hairpin-helix motif (PF00633; HMM-score: 47.7)
  HHH_5; Helix-hairpin-helix domain (PF14520; HMM-score: 33.4)
  T2SSK; Type II secretion system (T2SS), protein K (PF03934; HMM-score: 23.8)
  Ubiquitin (CL0072) SLBB; SLBB domain (PF10531; HMM-score: 21.5)
  HHH (CL0198) HHH_2; Helix-hairpin-helix motif (PF12826; HMM-score: 21.5)
  HHH_6; Helix-hairpin-helix motif (PF14579; HMM-score: 19.5)
  HHH_8; Helix-hairpin-helix domain (PF14716; HMM-score: 16.5)
  T2SSK_1st; Type II secretion system (T2SS), protein K (PF21687; HMM-score: 16.4)
  PsbU; Photosystem II 12 kDa extrinsic protein (PsbU) (PF06514; HMM-score: 15)
  H2TH (CL0303) H2TH_5; S13-like H2TH domain (PF22525; HMM-score: 14.9)
  HHH (CL0198) UPF0758_N; UPF0758 N-terminal (PF20582; HMM-score: 14.4)
  Aha1_BPI (CL0648) DUF1439; Protein of unknown function (DUF1439) (PF07273; HMM-score: 13.9)
  HHH (CL0198) RNA_pol_A_CTD; Bacterial RNA polymerase, alpha chain C terminal domain (PF03118; HMM-score: 13.2)
  PH (CL0266) FERM_C_FAK1; FAK1/PYK2, FERM domain C-lobe (PF21477; HMM-score: 12.8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.17
  • Cytoplasmic Membrane Score: 9.51
  • Cellwall Score: 0.16
  • Extracellular Score: 0.15
  • Internal Helix: 1
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0.0001
  • Cytoplasmic Membrane Score: 0.974
  • Cell wall & surface Score: 0.0018
  • Extracellular Score: 0.0241
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00159
  • TAT(Tat/SPI): 0.000223
  • LIPO(Sec/SPII): 0.001235
• predicted transmembrane helices (TMHMM): 1

⊟Accession numbers

• GI:
• RefSeq:
• UniProt:

⊟Protein sequence

• MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKNGNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• Operon-mapper  : holA < JSNZ_001578 < JSNZ_001579 < JSNZ_001580 < JSNZ_001581 < rsfS < yqeK < nadD < yhbY < aroE < yqeH < JSNZ_001588 < mtnN

⊟Regulation

• regulator: SigH* regulon

  SigH*

  (sigma factor)

  controlling competence and phage integrase genes; regulation predicted or transferred from N315 and NCTC 8325 in Wolfgramm et al. (https://doi.org/10.1101/2025.09.03.674026)   other strains

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications