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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

organism: Staphylococcus aureus JSNZ
locus tag: JSNZ_001586
pan locus tag^?: SAUPAN004182000
symbol: aroE
pan gene symbol^?: aroE
synonym:
product: shikimate dehydrogenase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

type: CDS
locus tag: JSNZ_001586
symbol: aroE
product: shikimate dehydrogenase
replicon: chromosome
strand: -
coordinates: 1621247..1622053
length: 807
essential: unknown other strains

⊟Accession numbers[edit | edit source]

Gene ID:
RefSeq:
BioCyc:
MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

1
61
121
181
241
301
361
421
481
541
601
661
721
781
ATGAAATTTGCAGTTATCGGAAATCCTATTTCACATTCCTTGTCGCCCGTTATGCATAGA
GCAAATTTTAATTCTTTAGGATTAGATGATACTTATGAAGCTTTAAATATTCCAATTGAA
GATTTTCATTTAATTAAAGAAATTATTTCGAAAAAAGAATTAGATGGCTTTAATATCACA
ATTCCTCATAAAGAACGTATCATACCGTATTTAGATCATGTTGATGAACAAGCGATTAAT
GCAGGTGCAGTTAACACTGTTTTGATAAAAGATGACAAGTGGATAGGGTATAATACAGAT
GGTATTGGTTATGTTAAAGGATTGCACAGCGTTTATCCAGATTTAGAAAATGCATACATT
TTAATTTTGGGCGCAGGTGGTGCAAGTAAAGGTATTGCTTATGAATTAGCAAAATTTGTA
AAGCCCAAATTAACTGTTGCGAATAGAACGATGGCTCGTTTTGAATCTTGGAATTTAAAT
ATAAACCAAATTTCATTAGCAGATGCTGAAAAGTATTTAGCTGAATTCGATATCGTTATT
AATACAACACCAGCGGGTATGGCTGGAAATAACGAAAGTATTATTAATTTAAAGCATCTT
TCTCCCAATACTTTAATGAGTGATATTGTTTATATACCGTATAAAACACCTATTTTAGAG
GAAGCAGAGCGCAAGGGAAACCATATTTATAATGGCTTAGATATGTTTGTTTACCAAGGT
GCGGAAAGCTTTAAAATTTGGACTAATAAAGATGCTGATATTAATTCTATGAAAACAGCA
GTTTTACAACAATTAAAAGGAGAATAA
60
120
180
240
300
360
420
480
540
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660
720
780
807

⊟Protein[edit | edit source]

⊟General[edit | edit source]

locus tag: JSNZ_001586
symbol: AroE
description: shikimate dehydrogenase
length: 268
theoretical pI: 5.58603
theoretical MW: 29897
GRAVY: -0.148881

⊟Function[edit | edit source]

reaction:
EC 1.1.1.25? ExPASy
Shikimate dehydrogenase Shikimate + NADP⁺ = 3-dehydroshikimate + NADPH
TIGRFAM:
Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate dehydrogenase (TIGR00507; EC 1.1.1.25; HMM-score: 262.4)
and 3 more
Metabolism Amino acid biosynthesis Aromatic amino acid family shikimate-5-dehydrogenase (TIGR01809; EC 1.1.1.25; HMM-score: 127.9)
Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamyl-tRNA reductase (TIGR01035; EC 1.2.1.70; HMM-score: 33.2)
Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, A subunit (TIGR02853; HMM-score: 20.6)
TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
PFAM:
AA_dh_N (CL0603) Shikimate_dh_N; Shikimate dehydrogenase substrate binding domain (PF08501; HMM-score: 105.7)
and 6 more
no clan defined SDH_C; Shikimate 5'-dehydrogenase C-terminal domain (PF18317; HMM-score: 47.8)
NADP_Rossmann (CL0063) Shikimate_DH; Shikimate / quinate 5-dehydrogenase (PF01488; HMM-score: 39.6)
Sacchrp_dh_NADP; Saccharopine dehydrogenase NADP binding domain (PF03435; HMM-score: 20.4)
2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 13.6)
ApbA; Ketopantoate reductase PanE/ApbA (PF02558; HMM-score: 13.3)
no clan defined DUF2682; Protein of unknown function (DUF2682) (PF10909; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

domains:
modifications:
cofactors:
effectors:

⊟Localization[edit | edit source]

PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9951
- Cytoplasmic Membrane Score: 0.0003
- Cell wall & surface Score: 0.0002
- Extracellular Score: 0.0044
LocateP:
SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.007519
- TAT(Tat/SPI): 0.000501
- LIPO(Sec/SPII): 0.001045
predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

GI:
RefSeq:
UniProt:

⊟Protein sequence[edit | edit source]

MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHLIKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDDKWIGYNTDGIGYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLNINQISLADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPILEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE

⊟Experimental data[edit | edit source]

experimentally validated: data available for COL, NCTC8325
protein localization: data available for COL
quantitative data / protein copy number per cell: data available for COL
interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

Operon-mapper ^[1] : holA < JSNZ_001578 < JSNZ_001579 < JSNZ_001580 < JSNZ_001581 < rsfS < yqeK < nadD < yhbY < aroE < yqeH < JSNZ_001588 < mtnN

⊟Regulation[edit | edit source]

regulator:

⊟Transcription pattern[edit | edit source]

S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

Aureolib: data available for COL

⊟Protein stability[edit | edit source]

half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
Bioinformatics: 2018, 34(23);4118-4120
[PubMed:29931111] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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