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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1509 [new locus tag: SA_RS08500 ]
  • pan locus tag?: SAUPAN004308000
  • symbol: nrdR
  • pan gene symbol?: nrdR
  • synonym:
  • product: transcriptional regulator NrdR

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1509 [new locus tag: SA_RS08500 ]
  • symbol: nrdR
  • product: transcriptional regulator NrdR
  • replicon: chromosome
  • strand: -
  • coordinates: 1718694..1719164
  • length: 471
  • essential: yes DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAAATGCCCGAAATGTAATTCTACACAATCTAAAGTTGTAGATTCAAGGCATGCCGAT
    GAATTAAATGCCATTCGAAGACGAAGAGAATGTGAAAATTGTGGAACACGTTTCACTACA
    TTTGAACATATCGAAGTTAGTCAGCTTATAGTTGTGAAAAAAGATGGCACAAGAGAGCAG
    TTTTCAAGAGAAAAAATACTTAATGGACTTGTGCGTTCTTGTGAGAAACGACCAGTTAGA
    TATCAACAACTTGAAGACATAACAAACAAGGTTGAATGGCAATTACGAGATGAAGGTCAT
    ACGGAAGTGTCTTCACGAGATATAGGTGAACACGTTATGAACTTGTTAATGCATGTTGAT
    CAAGTTTCATATGTTAGATTTGCATCAGTCTATAAAGAATTTAAAGATGTTGATCAATTG
    TTAGCATCGATGCAAGGGATTTTAAGCGAAAACAAACGGAGTGATGCTTAA
    60
    120
    180
    240
    300
    360
    420
    471

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1509 [new locus tag: SA_RS08500 ]
  • symbol: NrdR
  • description: transcriptional regulator NrdR
  • length: 156
  • theoretical pI: 7.89688
  • theoretical MW: 18203.5
  • GRAVY: -0.744872

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions transcriptional regulator NrdR (TIGR00244; HMM-score: 187.7)
    and 4 more
    Cys-rich peptide, TIGR04165 family (TIGR04165; HMM-score: 16.4)
    Signal transduction Regulatory functions DNA interactions putative regulatory protein, FmdB family (TIGR02605; HMM-score: 14.6)
    MJ0042 family finger-like domain (TIGR02098; HMM-score: 12)
    cxxc_20_cxxc protein (TIGR04104; HMM-score: 8.7)
  • TheSEED  :
    • Ribonucleotide reductase transcriptional regulator NrdR
    Nucleosides and Nucleotides Nucleosides and Nucleotides - no subcategory Ribonucleotide reduction  Ribonucleotide reductase transcriptional regulator NrdR
  • PFAM:
    no clan defined ATP-cone; ATP cone domain (PF03477; HMM-score: 65.1)
    and 11 more
    C2H2-zf (CL0361) zf-DBF; DBF zinc finger (PF07535; HMM-score: 19.1)
    Zn_Beta_Ribbon (CL0167) TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 16.3)
    Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 15.4)
    Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 14.6)
    zinc_ribbon_5; zinc-ribbon domain (PF13719; HMM-score: 14.1)
    zf-FYVE-PHD (CL0390) FYVE; FYVE zinc finger (PF01363; HMM-score: 14)
    Zn_Beta_Ribbon (CL0167) Elf1; Transcription elongation factor Elf1 like (PF05129; HMM-score: 13.8)
    TFIIS_C; Transcription factor S-II (TFIIS) (PF01096; HMM-score: 12.2)
    C2H2-zf (CL0361) zf-C2H2; Zinc finger, C2H2 type (PF00096; HMM-score: 11.1)
    Zn_Beta_Ribbon (CL0167) zinc_ribbon_2; zinc-ribbon domain (PF13240; HMM-score: 8.7)
    zinc_ribbon_4; zinc-ribbon domain (PF13717; HMM-score: 8.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effector: Deoxyribonucleotides
  • genes regulated by NrdR, TF important in Deoxyribonucleotide biosynthesisRegPrecise
    repression
    nrdI > nrdE > nrdF
    nrdG* < nrdD

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.016516
    • TAT(Tat/SPI): 0.00067
    • LIPO(Sec/SPII): 0.005624
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHIEVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTEVSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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