PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA1508 [new locus tag: SA_RS08495 ]
• pan locus tag^?: SAUPAN004307000
• symbol: dnaB
• pan gene symbol^?: dnaB
• synonym:
• product: chromosome replication initiation/membrane attachment protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA1508 [new locus tag: SA_RS08495 ]
• symbol: dnaB
• product: chromosome replication initiation/membrane attachment protein
• replicon: chromosome
• strand: -
• coordinates: 1717293..1718693
• length: 1401
• essential: no DEG other strains

⊟Accession numbers

• Gene ID: 1124353 NCBI
• RefSeq: NP_374796 NCBI
• BioCyc: see SA_RS08495
• MicrobesOnline: 103822 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGGGACGACAAGCCTTCGAATTCGGCTTAAGACCAAAGGATCAATTCAAAGTTATGCAA
  CACTTTGATTTAAATACGAATCATTTAGAGGTATTGAATCGACTGTATACACCGCTAATT
  GGAACGCAAGCAGTGGGTTTATATCATTTTATGACTCAATTCGTTAAAGACAGTCATAAT
  GAAACATTGATTCTTTCTCATTATATTTTTATGAATGAACTTAAAATTAATTTACTAGAG
  TTTAGACAACAAATGGACTTACTTGAAGCGATTGGATTGTTAAAGGCGTTTGTCAAACAT
  GATGAACAAGAAACACAATTTGTTTACCAATTAATTCAACCGCCTTCAGCACATTTATTT
  TTTAATGATCCAATGTTGTCAATCTTTTTATATAGTGAAGTCGAGCATCGTCGATTTCAT
  GAGTTGAAGAAATACTTTGAGTATCAACAAATTGATTTGAGTGAATTTAAACAGGTAACA
  AGACAATTTACTGATGTTTTTAAAGTGCCGTCTACAAAAATAGATATCGATACAAGTGAT
  ATTCCGATTAATGAACCGTACCAAGGTATCGATTTATCAAATGAATCTTTTGACTTTGAA
  ATGTTGAGACAAATGTTAGGTAAACATTTTATTAGTCAAGACATTGTTACCAAAGATGCA
  AAGCGTTTAATTACACAACTCGCGACTTTATATGGTTTAACAGCAGATGGCATGAAGCAT
  GTTATTTTAAATTCAATTACAAGTGGACAACAGTTATCCTTTGAAGAAATGCGTAAACAA
  GCACGCTCGTATTATTTAATGGAACACGAAAATCAAATGCCTAAATTACAGGTGAAATCT
  CCTGCTACATCATCTTCGACGGGCAAATCTTCAGAAGTGAATCCAAAGCCACAAAGTGAT
  GAATGGTTTGAGTTACTTGAACAAACGAGTCCAATTGATATGTTAGCTTCTTGGTCTGAA
  TCAGAACCAACTATATCGCAAAAGACAATGGTTGAAGAATTAATTGAACGTGAAAAAATG
  TCTTTTGGCGTCATTAATATTTTGTTGCAATTTGTCATGTTAAAAGAAGATATGAAACTT
  CCTAAAGCGTATATTTTGGAAATAGCATCAAATTGGAAGAAAAAAGGTATTAAGACAGCT
  AAAGAAGCATATAATTACGCTAAAAAAGTTAATCAACCTAAAAATGAGGGTTCTAGCGGA
  AATTACCAAAAGCGTGGTTCATACTATGGGCAACGTAATCGTATTTCAAAAGAAAAAACA
  CCTAAATGGTTAGAAAATAGAGATAAACCTAGTGAAGAAGATTCGGCTAAAGATAATAGC
  GTAGATGATCAACAATTAGAGCAAGATCGACAAGCATTTCTAGATAAATTATCTAAAAAA
  TGGGAGGAGGACAGTCGATAA
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⊟Protein

⊟General

• locus tag: SA1508 [new locus tag: SA_RS08495 ]
• symbol: DnaB
• description: chromosome replication initiation/membrane attachment protein
• length: 466
• theoretical pI: 6.26131
• theoretical MW: 54576.7
• GRAVY: -0.66867

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DnaD domain protein (TIGR01446; HMM-score: 20.3)
  Genetic information processing Mobile and extrachromosomal element functions Prophage functions DnaD domain protein (TIGR01446; HMM-score: 20.3)
• TheSEED  :
  • Replication initiation and membrane attachment protein BsDnaB
• PFAM:
  no clan defined DnaB_2; Replication initiation and membrane attachment (PF07261; HMM-score: 39.2)
  and 2 more
  DUF4144; protein structure with unknown function (PF13642; HMM-score: 14.6)
  SUIM_assoc; Unstructured region C-term to UIM in Ataxin3 (PF16619; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.002075
  • TAT(Tat/SPI): 0.000222
  • LIPO(Sec/SPII): 0.000239
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15927263 NCBI
• RefSeq: NP_374796 NCBI
• UniProt: A0A0H3JUI0 UniProt

⊟Protein sequence

• MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQAVGLYHFMTQFVKDSHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDEQETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVTRQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTKDAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKLQVKSPATSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEELIEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQPKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDRQAFLDKLSKKWEEDSR

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: dnaI < dnaB < nrdR

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications