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NCBI: 26-AUG-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1024 [new locus tag: SA_RS05835 ]
  • pan locus tag?: SAUPAN003451000
  • symbol: pbpA
  • pan gene symbol?: pbp1
  • synonym:
  • product: penicillin-binding protein 1

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SA1024 [new locus tag: SA_RS05835 ]
  • symbol: pbpA
  • product: penicillin-binding protein 1
  • replicon: chromosome
  • strand: +
  • coordinates: 1158054..1160288
  • length: 2235
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGAAGCAAAAAATTAAAATTAAAAAAAATAAAATAGGGGCAGTCCTACTTGTTGGT
    TTATTCGGACTGCTCTTTTTTATATTGGTTTTAAGAATTTCATATATCATGATTACTGGA
    CATTCTAATGGTCAAGATTTAGTCATGAAGGCAAATGAAAAGTATTTAGTTAAGAATGCA
    CAACAACCAGAACGAGGAAAGATATATGATCGTAATGGTAAAGTGCTAGCAGAAGATGTA
    GAAAGATATAAACTTGTTGCAGTAATAGATAAAAAGGCGAGTGCCAATTCTAAAAAACCT
    AGGCATGTAGTTGATAAAAAAGAGACTGCAAAGAAATTATCTACAGTCATTGATATGAAG
    CCAGAGGAAATTGAAAAGAGACTTAGTCAAAAGAAAGCTTTCCAAATTGAATTTGGACGC
    AAAGGAACAAATTTAACGTATCAGGACAAATTGAAAATAGAGAAAATGAATTTGCCTGGT
    ATTTCTTTATTGCCTGAAACAGAACGCTTTTATCCAAATGGCAATTTTGCATCACACTTA
    ATTGGTAGAGCTCAGAAAAATCCGGATACTGGTGAACTTAAAGGTGCACTTGGAGTTGAA
    AAGATTTTTGATAGTTATTTAAGTGGATCTAAAGGATCATTGAGATATATTCATGATATT
    TGGGGATATATCGCACCAAATACTAAAAAAGAGAAGCAGCCTAAACGTGGTGATGATGTC
    CATTTAACAATCGATTCAAATATTCAAGTATTTGTTGAAGAAGCTTTAGATGGCATGGTT
    GAAAGATACCAGCCGAAAGATTTATTTGCGGTTGTCATGGATGCCAAAACTGGAGAAATT
    TTAGCATACAGTCAGCGACCAACATTTAATCCTGAAACTGGTAAAGACTTTGGTAAAAAG
    TGGGCAAATGACCTTTATCAAAACACATACGAGCCTGGATCAACATTTAAATCATATGGG
    TTAGCAGCTGCTATTCAAGAAGGTGCTTTTGATCCTGATAAGAAATATAAATCTGGACAT
    AGAGATATTATGGGTTCACGTATTTCAGACTGGAATAGAGTCGGTTGGGGTGAAATCCCA
    ATGTCACTCGGATTTACTTATTCATCTAATACATTGATGATGCATTTACAAGATTTAGTT
    GGTGCAGACAAAATGAAATCTTGGTATGAACGATTTGGATTTGGAAAATCAACTAAAGGT
    ATGTTTGATGGAGAAGCACCTGGTCAAATTGGATGGAGTAATGAGTTGCAACAAAAAACG
    TCATCATTTGGTCAATCGACAACAGTAACACCTGTTCAAATGTTACAGGCGCAATCAGCG
    TTCTTTAATGATGGTAATATGTTAAAACCATGGTTTGTGAATAGCGTTGAAAATCCTGTT
    AGTAAAAGACAATTTTATAAAGGTCAAAAACAAATCGCAGGCAAACCAATAACAAAAGAT
    ACTGCTGAAAAAGTTGAAAAGCAATTGGATTTAGTTGTGAATAGTAAGAAGAGTCACGCT
    GCAAACTATCGTATTGATGGTTATGAGGTCGAAGGTAAGACTGGTACAGCACAAGTCGCT
    GCACCTAATGGTGGTGGATACGTTAAAGGTCCAAACCCATATTTTGTAAGTTTTATGGGT
    GACGCACCGAAGAAAAATCCTAAAGTTATTGTATACGCTGGTATGAGCTTGGCACAAAAA
    AATGACCAAGAAGCTTATGAATTAGGTGTTAGTAAAGCGTTTAAACCAATAATGGAAAAT
    ACTTTGAAATATTTAAATGTAGGTAAATCAAAAGATGACACATCTAATGCAGAGTATAGT
    AAAGTGCCAGATGTTGAAGGTCAAGACAAACAAAAAGCTATTGATAATGTGAGTGCAAAA
    TCATTAGAACCAGTTACTATCGGTTCTGGCACACAAATAAAAGCACAATCTATAAAAGCA
    GGGAATAAAGTCTTACCTCATAGTAAAGTACTGTTATTAACAGACGGAGACTTAACTATG
    CCTGACATGTCAGGATGGACGAAAGAAGATGTCATTGCTTTTGAAAACCTAACAAATATT
    AAAGTAAATTTAAAAGGTAGCGGTTTTGTGTCCCACCAATCAATTAGTAAGGGACAAAAA
    CTTACTGAAAAAGATAAAATAGACGTAGAATTTTCATCAGAGAATGTAGACAGCAATTCG
    ACGAATAATTCTGATTCAAATTCAGATGATAAGAAGAAATCTGACAGTAAAACTGACAAG
    GATAAGTCGGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2235

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SA1024 [new locus tag: SA_RS05835 ]
  • symbol: PbpA
  • description: penicillin-binding protein 1
  • length: 744
  • theoretical pI: 9.83098
  • theoretical MW: 82709.4
  • GRAVY: -0.643011

⊟Function[edit | edit source]

  • TIGRFAM:
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan stage V sporulation protein D (TIGR02214; HMM-score: 375.8)
    Cellular processes Cellular processes Sporulation and germination stage V sporulation protein D (TIGR02214; HMM-score: 375.8)
    and 3 more
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 2 (TIGR03423; HMM-score: 260.5)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein, 1A family (TIGR02074; HMM-score: 84.8)
    Cell structure Cell envelope Biosynthesis and degradation of murein sacculus and peptidoglycan penicillin-binding protein 1C (TIGR02073; HMM-score: 18)
  • TheSEED  :
    • Cell division protein FtsI, peptidoglycan D,D-transpeptidase (EC 3.4.16.4)
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)
    and 1 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Peptidoglycan Biosynthesis  Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129)
  • PFAM:
    Beta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 277)
    and 3 more
    no clan defined PBP_dimer; Penicillin-binding Protein dimerisation domain (PF03717; HMM-score: 81.5)
    PASTA; PASTA domain (PF03793; HMM-score: 65)
    DdrB; DdrB-like protein (PF12747; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.17
    • Cytoplasmic Membrane Score: 9.51
    • Cellwall Score: 0.16
    • Extracellular Score: 0.15
    • Internal Helices: 2
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.07004
    • TAT(Tat/SPI): 0.000531
    • LIPO(Sec/SPII): 0.004824
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDKDKSD

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:
    SA1234(cspA)cold-shock protein CspA  [1] (data from MRSA252)
    SA0505(fus)elongation factor G  [1] (data from MRSA252)
    SA2204(gpmA)phosphoglyceromutase  [1] (data from MRSA252)
    SA1305(hu)DNA-binding protein II  [1] (data from MRSA252)
    SA2400(mqo2)malate:quinone oxidoreductase  [1] (data from MRSA252)
    SA2334(mvaS)3-hydroxy-3-methylglutaryl-CoA synthase  [1] (data from MRSA252)
    SA1520(pykA)pyruvate kinase  [1] (data from MRSA252)
    SA0496(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    SA2044(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    SA2047(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    SA2046(rplD)50S ribosomal protein L4  [1] (data from MRSA252)
    SA0498(rplL)50S ribosomal protein L7/L12  [1] (data from MRSA252)
    SA2029(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SA2040(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    SA1473(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    SA2042(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    SAS052(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    SA2031(rpsE)30S ribosomal protein S5  [1] (data from MRSA252)
    SA2038(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    SA2043(rpsS)30S ribosomal protein S19  [1] (data from MRSA252)
    SA1528hypothetical protein  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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