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⊟Summary[edit | edit source]

  • pan ID?: SAUPAN003451000
  • symbol?: pbp1
  • synonym:
  • description?: penicillin-binding protein

      descriptions from strain specific annotations:

    • penicillin-binding protein
    • penicillin-binding protein 1
    • cell division protein FtsI
    • Cell division protein FtsI (Peptidoglycan synthetase)
    • penicillin-binding protein 2B (PBP-2B)
    • penicillin binding transpeptidase domain protein
  • strand?: +
  • coordinates?: 3793808..3796042
  • synteny block?: BlockID0025310
  • occurrence?: in 100% of 34 strains

pbpA (pbp1) : penicillin-binding protein 1 [1]

• A crystal structure is available : 8VBT

Staphylococci possess either four or five "penicillin-binding protein" peptidoglycan transpeptidases. PBP1 is a class B high molecular weight PBP with multiple C-terminal PASTA domains and an N-terminal transpeptidase domain. It localizes to the nascent septum of dividing cells where it coordinates formation of a mature cell divisome complex and establishment of the septal plate via its C-terminal PASTA domains. Its N-terminal transpeptidase activity is also required for proper crosslinking of septal peptidoglycan which, in the absence of PBP1 crosslinking, continues to grow resulting in thickened and aberrantly placed septa. Cloxacillin and carbapenems display more selective inhibition of PBP1 transpeptidation than of other staphylococcal PBPs. PBP1 specifically associates with its partner peptidoglycan transglycosylase FtsW to polymerize peptidoglycan disaccharides. Finally, inhibition of PBP1 transpeptidation can enhance daptomycin activity suggesting a functional role for daptomycin at the nascent cell septum.

⊟Orthologs[edit | edit source]

    COL:
    SACOL1194 (pbp1)
    N315:
    SA1024 (pbpA)
    NCTC8325:
    SAOUHSC_01145
    Newman:
    NWMN_1091 (pbpA)
    USA300_FPR3757:
    SAUSA300_1075 (pbpA)
    04-02981:
    SA2981_1139 (ftsI)
    08BA02176:
    C248_1210 (pbpA)
    11819-97:
    MS7_1138
    6850:
    RSAU_001062 (pbp1)
    71193:
    ST398NM01_1178
    ECT-R 2:
    ECTR2_1037
    ED133:
    SAOV_1182
    ED98:
    SAAV_1152 (pbp1)
    HO 5096 0412:
    SAEMRSA15_10140 (pbpA)
    JH1:
    SaurJH1_1265
    JH9:
    SaurJH9_1240
    JKD6008:
    SAA6008_01135 (pbpA)
    JKD6159:
    SAA6159_01032 (pbpA)
    JSNZ:
    JSNZ_001150
    LGA251:
    SARLGA251_10920 (pbpA)
    M013:
    M013TW_1115
    MRSA252:
    SAR1157 (pbpA)
    MSHR1132:
    SAMSHR1132_10230
    MSSA476:
    SAS1115
    Mu3:
    SAHV_1171 (pbpA)
    Mu50:
    SAV1181 (pbpA)
    MW2:
    MW1064 (pbpA)
    RF122:
    SAB1045 (pbpA)
    ST398:
    SAPIG1178
    T0131:
    SAT0131_01226 (rpsS)
    TCH60:
    HMPREF0772_12051 (pbp1)
    TW20:
    SATW20_11750 (pbpA)
    USA300_TCH1516:
    USA300HOU_1121 (pbpA)
    VC40:
    SAVC_05070

⊟Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757

⊟Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.307)


    COL             MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNA
    N315            MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNA
    NCTC8325        MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNA
    Newman          MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNA
    USA300_FPR3757  MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNGQDLVMKANEKYLVKNA
                    ************************************************************

    COL             QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMK
    N315            QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVIDMK
    NCTC8325        QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMK
    Newman          QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMK
    USA300_FPR3757  QQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRHVVDKKETAKKLSTVINMK
                    *********************************************************:**

    COL             PEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHL
    N315            PEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHL
    NCTC8325        PEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHL
    Newman          PEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHL
    USA300_FPR3757  PEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPGISLLPETERFYPNGNFASHL
                    ************************************************************

    COL             IGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
    N315            IGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
    NCTC8325        IGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
    Newman          IGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
    USA300_FPR3757  IGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIHDIWGYIAPNTKKEKQPKRGDDV
                    ************************************************************

    COL             HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK
    N315            HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK
    NCTC8325        HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK
    Newman          HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK
    USA300_FPR3757  HLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAKTGEILAYSQRPTFNPETGKDFGKK
                    ************************************************************

    COL             WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIP
    N315            WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIP
    NCTC8325        WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIP
    Newman          WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIP
    USA300_FPR3757  WANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDKKYKSGHRDIMGSRISDWNRVGWGEIP
                    ************************************************************

    COL             MSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKT
    N315            MSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKT
    NCTC8325        MSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKT
    Newman          MSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKT
    USA300_FPR3757  MSLGFTYSSNTLMMHLQDLVGADKMKSWYERFGFGKSTKGMFDGEAPGQIGWSNELQQKT
                    ************************************************************

    COL             SSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
    N315            SSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
    NCTC8325        SSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
    Newman          SSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
    USA300_FPR3757  SSFGQSTTVTPVQMLQAQSAFFNDGNMLKPWFVNSVENPVSKRQFYKGQKQIAGKPITKD
                    ************************************************************

    COL             TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMG
    N315            TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMG
    NCTC8325        TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMG
    Newman          TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMG
    USA300_FPR3757  TAEKVEKQLDLVVNSKKSHAANYRIDGYEVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMG
                    ************************************************************

    COL             DAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYS
    N315            DAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYS
    NCTC8325        DAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYS
    Newman          DAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYS
    USA300_FPR3757  DAPKKNPKVIVYAGMSLAQKNDQEAYELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYS
                    ************************************************************

    COL             KVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTM
    N315            KVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTM
    NCTC8325        KVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTM
    Newman          KVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTM
    USA300_FPR3757  KVPDVEGQDKQKAIDNVSAKSLEPVTIGSGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTM
                    ************************************************************

    COL             PDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
    N315            PDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
    NCTC8325        PDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
    Newman          PDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
    USA300_FPR3757  PDMSGWTKEDVIAFENLTNIKVNLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNS
                    ************************************************************

    COL             TNNSDSNSDDKKKSDSKTDKDKSD
    N315            TNNSDSNSDDKKKSDSKTDKDKSD
    NCTC8325        TNNSDSNSDDKKKSDSKTDKDKSD
    Newman          TNNSDSNSDDKKKSDSKTDKDKSD
    USA300_FPR3757  TNNSDSNSDDKKKSDSKTDKDKSD
                    ************************

  1. ↑ S F F Pereira, A O Henriques, M G Pinho, H de Lencastre, A Tomasz
    Role of PBP1 in cell division of Staphylococcus aureus.
    J Bacteriol: 2007, 189(9);3525-31
    [PubMed:17307860] [WorldCat.org] [DOI] (P p)    Siseth Martínez-Caballero, Kiran V Mahasenan, Choon Kim, Rafael Molina, Rhona Feltzer, Mijoon Lee, Renee Bouley, Dusan Hesek, Jed F Fisher, Inés G Muñoz, Mayland Chang, Shahriar Mobashery, Juan A Hermoso
    Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery.
    Comput Struct Biotechnol J: 2021, 19;5392-5405
    [PubMed:34667534] [WorldCat.org] [DOI] (P e)    Katarzyna Wacnik, Vincenzo A Rao, Xinyue Chen, Lucia Lafage, Manuel Pazos, Simon Booth, Waldemar Vollmer, Jamie K Hobbs, Richard J Lewis, Simon J Foster
    Penicillin-Binding Protein 1 (PBP1) of Staphylococcus aureus Has Multiple Essential Functions in Cell Division.
    mBio: 2022, 13(4);e0066922
    [PubMed:35703435] [WorldCat.org] [DOI] (I p)    Maria D Barbuti, Ine S Myrbråten, Danae Morales Angeles, Morten Kjos
    The cell cycle of Staphylococcus aureus: An updated review.
    Microbiologyopen: 2023, 12(1);e1338
    [PubMed:36825883] [WorldCat.org] [DOI] (I p)
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