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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_2530 [new locus tag: NWMN_RS14540 ]
  • pan locus tag?: SAUPAN006359000
  • symbol: NWMN_2530
  • pan gene symbol?: arcR
  • synonym:
  • product: transcriptional regulator Crp/Fnr family protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_2530 [new locus tag: NWMN_RS14540 ]
  • symbol: NWMN_2530
  • product: transcriptional regulator Crp/Fnr family protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2783544..2784248
  • length: 705
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACAGAAAACTTTATTTTGGGTAGAAATAATAAATTAGAACATGAACTAAAGGCATTA
    GCAGATTACATTAATATACCATATAGTATATTACAACCATATCAAAGTGAATGTTTTGTC
    AGACATTATACGAAAGGCCAAGTTATTTATTTTTCGCCACAAGAAAGTAGCAATATTTAC
    TTTTTAATTGAAGGTAACATTATTAGAGAACATTACAATCAAAATGGAGATGTATATCGT
    TATTTTAATAAAGAGCAAGTATTATTTCCAATCAGTAACTTATTTCATCCGAAAGAGGTT
    AACGAATTGTGTACAGCATTAACCGATTGTACAGTTCTTGGATTGCCTAGAGAATTGATG
    GCCTTTTTGTGCAAAGCTAATGATGATATATTTTTGACACTTTTTGCATTAATAAATGAT
    AATGAGCAGCAACACATGAACTATAACATGGCATTAACAAGTAAATTTGCTAAAGATCGA
    ATTATCAAATTGATATGCCATCTATGTCAGACAGTAGGATACGATCAAGATGAATTTTAT
    GAAATCAAACAGTTTTTAACTATTCAACTCATGAGTGATATGGCTGGGATTTCCCGGGAA
    ACAGCTGGTCATATTATTCATGAACTTAAAGATGAAAAACTTGTTGTTAAAGATCATAAA
    AATTGGTTAGTAAGCAAACATTTATTCAATGATGTATGTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_2530 [new locus tag: NWMN_RS14540 ]
  • symbol: NWMN_2530
  • description: transcriptional regulator Crp/Fnr family protein
  • length: 234
  • theoretical pI: 5.97765
  • theoretical MW: 27428.3
  • GRAVY: -0.217949

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions global nitrogen regulator NtcA (TIGR03697; HMM-score: 26.3)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 29.6)
    Cupin (CL0029) cNMP_binding; Cyclic nucleotide-binding domain (PF00027; HMM-score: 28.3)
    and 1 more
    no clan defined Catalase-rel; Catalase-related immune-responsive (PF06628; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Arginine
  • genes regulated by ArcR*, TF important in Arginine degradationRegPrecise
    activation
    arcR* < arcC < arcD < arcB < arcA
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004428
    • TAT(Tat/SPI): 0.000272
    • LIPO(Sec/SPII): 0.000783
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYTKGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNELCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDRIIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKDHKNWLVSKHLFNDVCV

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for NCTC8325
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, ArcR* (activation) regulon, ArgR* (repression) regulon, CcpA regulon
    Rex*(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    ArcR*(TF)important in Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    ArgR*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CcpA(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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