PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_2566 [new locus tag: SAUSA300_RS14275 ]
• pan locus tag^?: SAUPAN006359000
• symbol: arcR
• pan gene symbol^?: arcR
• synonym:
• product: Crp/FNR family transcriptional regulator

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_2566 [new locus tag: SAUSA300_RS14275 ]
• symbol: arcR
• product: Crp/FNR family transcriptional regulator
• replicon: chromosome
• strand: -
• coordinates: 2777389..2778093
• length: 705
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914440 NCBI
• RefSeq: YP_495200 NCBI
• BioCyc: see SAUSA300_RS14275
• MicrobesOnline: 1294081 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACAGAAAACTTTATTTTGGGTAGAAATAATAAATTAGAACATGAACTAAAGGCATTA
  GCAGATTACATTAATATACCATATAGTATATTACAACCATATCAAAGTGAATGTTTTGTC
  AGACATTATACGAAAGGCCAAGTTATTTATTTTTCGCCACAAGAAAGTAGCAATATTTAC
  TTTTTAATTGAAGGTAACATTATTAGAGAACATTACAATCAAAATGGAGATGTATATCGT
  TATTTTAATAAAGAGCAAGTATTATTTCCAATCAGTAACTTATTTCATCCGAAAGAGGTT
  AACGAATTGTGTACAGCATTAACCGATTGTACAGTTCTTGGATTGCCTAGAGAATTGATG
  GCCTTTTTGTGCAAAGCTAATGATGATATATTTTTGACACTTTTTGCATTAATAAATGAT
  AATGAGCAGCAACACATGAACTATAACATGGCATTAACAAGTAAATTTGCTAAAGATCGA
  ATTATCAAATTGATATGCCATCTATGTCAGACAGTAGGATACGATCAAGATGAATTTTAT
  GAAATCAAACAGTTTTTAACTATTCAACTCATGAGTGATATGGCTGGGATTTCCCGGGAA
  ACAGCTGGTCATATTATTCATGAACTTAAAGATGAAAAACTTGTTGTTAAAGATCATAAA
  AATTGGTTAGTAAGCAAACATTTATTCAATGATGTATGTGTTTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_2566 [new locus tag: SAUSA300_RS14275 ]
• symbol: ArcR
• description: Crp/FNR family transcriptional regulator
• length: 234
• theoretical pI: 5.97765
• theoretical MW: 27428.3
• GRAVY: -0.217949

⊟Function

• TIGRFAM:
  Signal transduction Regulatory functions DNA interactions global nitrogen regulator NtcA (TIGR03697; HMM-score: 26.3)
• TheSEED  :
  • Transcriptional regulator ArcR essential for anaerobic expression of the ADI pathway, Crp/Fnr family
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Transcriptional regulator ArcR essential for anaerobic expression of the ADI pathway, Crp/Fnr family
  and 1 more
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Transcriptional regulator ArcR essential for anaerobic expression of the ADI pathway, Crp/Fnr family
• PFAM:
  HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 29.6)
  Cupin (CL0029) cNMP_binding; Cyclic nucleotide-binding domain (PF00027; HMM-score: 28.3)
  and 1 more
  no clan defined Catalase-rel; Catalase-related immune-responsive (PF06628; HMM-score: 13.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effector: Arginine
• genes regulated by ArcR, TF important in Arginine degradationRegPrecise

  activation

  arcA

  arcR < arcC < arcD < arcB < arcA

  transcription units transferred from N315 data RegPrecise

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 2.5
  • Cytoplasmic Membrane Score: 2.5
  • Cellwall Score: 2.5
  • Extracellular Score: 2.5
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004428
  • TAT(Tat/SPI): 0.000272
  • LIPO(Sec/SPII): 0.000783
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161675 NCBI
• RefSeq: YP_495200 NCBI
• UniProt: Q2FDM8 UniProt

⊟Protein sequence

• MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYTKGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNELCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDRIIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKDHKNWLVSKHLFNDVCV

⊟Experimental data

• experimentally validated: data available for NCTC8325
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulators: ArcR (activation) regulon, ArgR (repression) regulon, CcpA regulon, Rex (repression) regulon

  ArcR	(TF)	important in Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise
  ArgR	(TF)	important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise
  CcpA	(TF)	important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise
  Rex	(TF)	important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications