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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02964
  • pan locus tag?: SAUPAN006359000
  • symbol: SAOUHSC_02964
  • pan gene symbol?: arcR
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02964
  • symbol: SAOUHSC_02964
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2725951..2726655
  • length: 705
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGACAGAAAACTTTATTTTGGGTAGAAATAATAAATTAGAACATGAACTAAAGGCATTA
    GCAGATTACATTAATATACCATATAGTATATTACAACCATATCAAAGTGAATGTTTTGTC
    AGACATTATACGAAAGGCCAAGTTATTTATTTTTCGCCACAAGAAAGTAGCAATATTTAC
    TTTTTAATTGAAGGTAACATTATTAGAGAACATTACAATCAAAATGGAGATGTATATCGT
    TATTTTAATAAAGAGCAAGTATTATTTCCAATCAGTAACTTATTTCATCCGAAAGAGGTT
    AACGAATTGTGTACAGCATTAACCGATTGTACAGTTCTTGGATTGCCTAGAGAATTGATG
    GCCTTTTTGTGCAAAGCTAATGATGATATATTTTTGACACTTTTTGCATTAATAAATGAT
    AATGAGCAGCAACACATGAACTATAACATGGCATTAACAAGTAAATTTGCTAAAGATCGA
    ATTATCAAATTGATATGCCATCTATGTCAGACAGTAGGATACGATCAAGATGAATTTTAT
    GAAATCAAACAGTTTTTAACTATTCAACTCATGAGTGATATGGCTGGGATTTCCCGGGAA
    ACAGCTGGTCATATTATTCATGAACTTAAAGATGAAAAACTTGTTGTTAAAGATCATAAA
    AATTGGTTAGTAAGCAAACATTTATTCAATGATGTATGTGTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    705

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02964
  • symbol: SAOUHSC_02964
  • description: hypothetical protein
  • length: 234
  • theoretical pI: 5.97765
  • theoretical MW: 27428.3
  • GRAVY: -0.217949

⊟Function[edit | edit source]

  • TIGRFAM:
    Signal transduction Regulatory functions DNA interactions global nitrogen regulator NtcA (TIGR03697; HMM-score: 26.3)
  • TheSEED  :
    • Transcriptional regulator ArcR essential for anaerobic expression of the ADI pathway, Crp/Fnr family
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Transcriptional regulator ArcR essential for anaerobic expression of the ADI pathway, Crp/Fnr family
    and 1 more
    Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Transcriptional regulator ArcR essential for anaerobic expression of the ADI pathway, Crp/Fnr family
  • PFAM:
    HTH (CL0123) HTH_Crp_2; Crp-like helix-turn-helix domain (PF13545; HMM-score: 29.6)
    Cupin (CL0029) cNMP_binding; Cyclic nucleotide-binding domain (PF00027; HMM-score: 28.3)
    and 1 more
    no clan defined Catalase-rel; Catalase-related immune-responsive (PF06628; HMM-score: 13.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effector: Arginine
  • genes regulated by ArcR*, TF important in Arginine degradationRegPrecise
    activation
    arcR* < arcC* < arcD* < arcB* < arcA*
    transcription unit transferred from N315 data RegPrecise

⊟Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.004428
    • TAT(Tat/SPI): 0.000272
    • LIPO(Sec/SPII): 0.000783
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYTKGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNELCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDRIIKLICHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKDHKNWLVSKHLFNDVCV

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: ArgR/AhrC* (repression) regulon, CcpA* regulon, Rex* (repression) regulon, ArcR* (activation) regulon
    ArgR/AhrC*(TF)important in Arginine biosynthesis, Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    CcpA*(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    Rex*(TF)important in Energy metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    ArcR*(TF)important in Arginine degradation; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Julia Makhlin, Tzili Kofman, Ilya Borovok, Christian Kohler, Susanne Engelmann, Gerald Cohen, Yair Aharonowitz
Staphylococcus aureus ArcR controls expression of the arginine deiminase operon.
J Bacteriol: 2007, 189(16);5976-86
[PubMed:17557828] [WorldCat.org] [DOI] (P p)

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