PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0057 [new locus tag: NWMN_RS00325 ]
• pan locus tag^?: SAUPAN000911000
• symbol: sirC
• pan gene symbol^?: sirC
• synonym:
• product: siderophore compound ABC transporter permease SirC

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0057 [new locus tag: NWMN_RS00325 ]
• symbol: sirC
• product: siderophore compound ABC transporter permease SirC
• replicon: chromosome
• strand: -
• coordinates: 76496..77494
• length: 999
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332435 NCBI
• RefSeq: YP_001331092 NCBI
• BioCyc:
• MicrobesOnline: 3705588 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGACCGAAAAGATTAATAAAAAAGACAATTACCATCTCATCTTCGCGTTAATCTTTTTA
  GCCATCGTTTCAGTGGTAAGTATGATGATTGGTTCAAGCTTTATACCATTACAACGCGTA
  CTGATGTACTTTATAAATCCAAATGACAGTATGGATCAATTCACTTTAGAAGTATTACGC
  TTACCTCGCATTACACTTGCGATTTTAGCAGGTGCCGCACTAGGAATGAGTGGTTTAATG
  TTGCAAAATGTATTAAAAAATCCAATTGCCTCACCTGATATTATCGGTATCACAGGTGGT
  GCTAGCTTAAGTGCTGTTGTCTTTATTGCATTTTTCAGCCATTTAACAATACATTTACTT
  CCACTATTTGCAGTATTAGGTGGCGCAGTTGCAATGATGATACTATTAGTGTTTCAAACG
  AAAGGACAAATACGCCCGACAACACTCATAATCATCGGTATTTCGATGCAAACGTTGTTT
  ATTGCGCTTGTCCAAGGATTACTCATTACAACGAAGCAATTATCTGCTGCCAAAGCTTAT
  ACATGGCTAGTCGGAAGTCTTTACGGTGCTACGTTTAAAGATACAATCATTTTGGGTATG
  GTTATTTTAGCTGTTGTGCCGTTGTTATTTCTTGTTATACCAAAAATGAAAATATCTATA
  CTTGATGACCCTGTAGCGATTGGCTTAGGCTTACATGTACAACGTATGAAACTAATCCAA
  TTAATCACTTCTACTATACTCGTATCTATGGCAATCAGTTTAGTAGGTAACATTGGGTTT
  GTCGGTTTAATCGCACCACATATCGCGAAAACAATCGTTCGCGGAAGTTATGCTAAAAAG
  TTACTAATGTCAGCAATGATTGGTGCCATATCAATTGTTATTGCAGACTTAATTGGGCGT
  ACCTTATTCTTGCCTAAAGAAGTGCCAGCAGGTGTATTTATTGCTGCTTTTGGTGCCCCA
  TTCTTCATATACTTATTATTAACCGTGAAAAAGTTATAA
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⊟Protein

⊟General

• locus tag: NWMN_0057 [new locus tag: NWMN_RS00325 ]
• symbol: SirC
• description: siderophore compound ABC transporter permease SirC
• length: 332
• theoretical pI: 10.6652
• theoretical MW: 35729.5
• GRAVY: 1.11657

⊟Function

• TIGRFAM:
  proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 149.6)
  and 1 more
  Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 27.5)
• TheSEED  :
  • Siderophore staphylobactin ABC transporter, permease protein SirC
  Iron acquisition and metabolism Siderophores Siderophore Staphylobactin  Siderophore staphylobactin ABC transporter, permease protein SirC
• PFAM:
  Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 283.1)
  and 3 more
  ABC-3; ABC 3 transport family (PF00950; HMM-score: 20.6)
  NADP_Rossmann (CL0063) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 13.9)
  no clan defined DUF4516; Domain of unknown function (DUF4516) (PF14990; HMM-score: 8)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9999
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0001
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.029257
  • TAT(Tat/SPI): 0.000776
  • LIPO(Sec/SPII): 0.016007
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: 151220269 NCBI
• RefSeq: YP_001331092 NCBI
• UniProt: A0A0H3KDB0 UniProt

⊟Protein sequence

• MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYFINPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGASLSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLFIALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKISILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGSYAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sirC < sirB < sirA

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)