PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0059 [new locus tag: NWMN_RS00335 ]
• pan locus tag^?: SAUPAN000913000
• symbol: sirA
• pan gene symbol^?: sirA
• synonym:
• product: siderophore compound ABC transporter binding protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0059 [new locus tag: NWMN_RS00335 ]
• symbol: sirA
• product: siderophore compound ABC transporter binding protein
• replicon: chromosome
• strand: -
• coordinates: 78502..79494
• length: 993
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332488 NCBI
• RefSeq: YP_001331094 NCBI
• BioCyc:
• MicrobesOnline: 3705590 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATAAAGTAATTAAAATGCTTGTTGTTACGCTTGCTTTCCTACTTGTTTTAGCAGGA
  TGTAGTGGGAATTCAAATAAACAATCATCTGATAACAAAGATAAGGAAACAACTTCAATT
  AAACATGCAATGGGTACAACTGAAATTAAAGGGAAACCAAAGCGTGTTGTTACGCTATAT
  CAAGGTGCCACTGACGTCGCTGTATCTTTAGGTGTTAAACCTGTAGGTGCTGTAGAATCA
  TGGACACAAAAACCGAAATTCGAATACATAAAAAATGATTTAAAAGATACTAAGATTGTA
  GGTCAAGAACCTGCACCTAACTTAGAGGAAATCTCTAAATTAAAACCGGACTTAATTGTC
  GCGTCAAAAGTTAGAAATGAAAAAGTTTACGATCAATTATCTAAAATCGCACCAACAGTT
  TCTACTGATACAGTTTTCAAATTCAAAGATACAACTAAGTTAATGGGGAAAGCTTTAGGG
  AAAGAAAAAGAAGCTGAAGATTTACTTAAAAAGTACGATGATAAAGTAGCTGCATTCCAA
  AAAGATGCAAAAGCAAAGTATAAAGATGCATGGCCATTGAAAGCTTCAGTTGTTAACTTC
  CGTGCTGATCATACAAGAATTTATGCTGGTGGATATGCTGGTGAAATCTTAAATGATTTA
  GGATTCAAACGTAATAAAGACTTACAAAAACAAGTTGATAATGGTAAAGATATTATCCAA
  CTTACATCTAAAGAAAGCATTCCATTAATGAACGCTGATCATATTTTTGTAGTAAAATCA
  GATCCAAATGCGAAAGATGCTGCATTAGTTAAAAAGACTGAAAGCGAATGGACTTCAAGT
  AAAGAGTGGAAAAATTTAGACGCAGTTAAAAACAACCAAGTATCTGATGATTTAGATGAA
  ATCACTTGGAACTTAGCTGGCGGATATAAATCTTCATTAAAACTTATTGACGATTTATAT
  GAAAAGTTAAATATTGAAAAACAATCAAAATAA
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⊟Protein

• 3MWF
  PDB
• 3MWG
  PDB

Protein Data Bank: 3MWF

Protein Data Bank: 3MWG

⊟General

• locus tag: NWMN_0059 [new locus tag: NWMN_RS00335 ]
• symbol: SirA
• description: siderophore compound ABC transporter binding protein
• length: 330
• theoretical pI: 9.7617
• theoretical MW: 36743.9
• GRAVY: -0.563636

⊟Function

• TIGRFAM:
  heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 61.7)
  and 5 more
  putative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 30.5)
  Cellular processes Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 16)
  proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 14.2)
  Metabolism Central intermediary metabolism Sulfur metabolism phosphoadenosine phosphosulfate reductase (TIGR02057; EC 1.8.4.8; HMM-score: 14)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking outer membrane assembly lipoprotein YfiO (TIGR03302; HMM-score: 13.2)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 89.6)
  and 1 more
  no clan defined ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.68
  • Cellwall Score: 0.17
  • Extracellular Score: 0.16
  • Internal Helix: 1
• LocateP: Lipid anchored
  • Prediction by SwissProt Classification: Extracellular
  • Pathway Prediction: Sec-(SPII)
  • Intracellular possibility: -0.33
  • Signal peptide possibility: 1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: LVLAGCS
• SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
  • SP(Sec/SPI): 0.000361
  • TAT(Tat/SPI): 0.000102
  • LIPO(Sec/SPII): 0.999306
  • Cleavage Site: CS pos: 20-21. LAG-CS. Pr: 0.9999
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151220271 NCBI
• RefSeq: YP_001331094 NCBI
• UniProt: A0A0H3K629 UniProt

⊟Protein sequence

• MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sirC < sirB < sirA

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)