PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0058 [new locus tag: NWMN_RS00330 ]
• pan locus tag^?: SAUPAN000912000
• symbol: sirB
• pan gene symbol^?: sirB
• synonym:
• product: siderophore compound ABC transporter permease SirB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0058 [new locus tag: NWMN_RS00330 ]
• symbol: sirB
• product: siderophore compound ABC transporter permease SirB
• replicon: chromosome
• strand: -
• coordinates: 77491..78486
• length: 996
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332489 NCBI
• RefSeq: YP_001331093 NCBI
• BioCyc:
• MicrobesOnline: 3705589 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGCTACTTAAACCAAAATACCAAATCGTTATTGCTGGTTTATGTCTTGCAATAGTAGCT
  ATCTTAAGTTTAATGATTGGAAATACGCTTGTGTCACCAGGTACGGTGATACAGGCGTTA
  TTCAACTTTGATAGTGAAAACGATTTACATGATGTTGTCACTGGTGCACGGGCGTCGAGA
  ACAATCATTGCGTTATTGACTGGTGCTGCCCTTGCTGTCTCAGGTTTGTTGATGCAAGCA
  CTTACACGAAACCCAATAGCCTCACCAGGGCTTTTCGGTGTCAATGCAGGCGCAGTATTT
  TTTGTCATTTTTAGTATTACATTTATCCAAATTCAATCTTTTAAAATGATTGTAGTTATT
  GCATTTTTGGGGGCTATTGTTGTTACTGTATTAGTTGTTGCACTAGGTATGTTTAGACAA
  ACACTATTCTCACCTCACCGTGTCATTTTGGCAGGTGCTGCGATTGCGATGCTATTTACA
  GCCTTTACTCAAGGCATACTTATTATGAACGAAACAGACTTACAAGGCCTATTATTTTGG
  TTAAGTGGCTCCGTTTCATTACGTAATATTTGGGATATCCCATGGATTATTCCGCTTGTA
  TTGATACTTATTTTAATTGCATTTAGCATGGCTGCACACATCAACATCTTGATGACAAGT
  GACGACATTGCAACCGGCCTCGGTCAAAACATAAAATTAATCAAATGGATGATTATTATG
  CTCATCAGTATGTTAGCCGGTATTTCGGTAGCCGTAGCTGGATCAATCGTCTTTGTGGGT
  CTTATCGTACCGAATATTAGCAAACGATTATTACCACCAAACTATAAGTATTTAATTCCT
  TTTACTGCATTAGCTGGAGCAATCCTAATGATCATTTCAGACATTGTTGCTCGTATAATA
  ATTAAGCCACTAGAGTTGCCTATCGGTGTCGTTACCGCTGTCATTGGCGCTATTGTCTTA
  ATCTATATTATGAAGAAAGGACGTCAACGCTTATGA
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⊟Protein

⊟General

• locus tag: NWMN_0058 [new locus tag: NWMN_RS00330 ]
• symbol: SirB
• description: siderophore compound ABC transporter permease SirB
• length: 331
• theoretical pI: 10.5981
• theoretical MW: 35525.2
• GRAVY: 1.22991

⊟Function

• TIGRFAM:
  proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 153.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 298.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.24136
  • TAT(Tat/SPI): 0.001711
  • LIPO(Sec/SPII): 0.027406
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: 151220270 NCBI
• RefSeq: YP_001331093 NCBI
• UniProt: A0A0H3K5J6 UniProt

⊟Protein sequence

• MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFDSENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFVIFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFTAFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILMTSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYKYLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sirC < sirB < sirA

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)