PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0116 [new locus tag: SAUSA300_RS00600 ]
• pan locus tag^?: SAUPAN000912000
• symbol: sirB
• pan gene symbol^?: sirB
• synonym:
• product: iron compound ABC transporter permease SirB

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0116 [new locus tag: SAUSA300_RS00600 ]
• symbol: sirB
• product: iron compound ABC transporter permease SirB
• replicon: chromosome
• strand: -
• coordinates: 132230..133225
• length: 996
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914429 NCBI
• RefSeq: YP_492833 NCBI
• BioCyc: see SAUSA300_RS00600
• MicrobesOnline: 1291631 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGCTACTTAAACCAAAATACCAAATCGTTATTGCTGGTTTATGTCTTGCAATAGTAGCT
  ATCTTAAGTTTAATGATTGGAAATACGCTTGTGTCACCAGGTACGGTGATACAGGCGTTA
  TTCAACTTTGATAGTGAAAACGATTTACATGATGTTGTCACTGGTGCACGGGCGTCGAGA
  ACAATCATTGCGTTATTGACTGGTGCTGCCCTTGCTGTCTCAGGTTTGTTGATGCAAGCA
  CTTACACGAAACCCAATAGCCTCACCAGGGCTTTTCGGTGTCAATGCAGGCGCAGTATTT
  TTTGTCATTTTTAGTATTACATTTATCCAAATTCAATCTTTTAAAATGATTGTAGTTATT
  GCATTTTTGGGGGCTATTGTTGTTACTGTATTAGTTGTTGCACTAGGTATGTTTAGACAA
  ACACTATTCTCACCTCACCGTGTCATTTTGGCAGGTGCTGCGATTGCGATGCTATTTACA
  GCCTTTACTCAAGGCATACTTATTATGAACGAAACAGACTTACAAGGCCTATTATTTTGG
  TTAAGTGGCTCCGTTTCATTACGTAATATTTGGGATATCCCATGGATTATTCCGCTTGTA
  TTGATACTTATTTTAATTGCATTTAGCATGGCTGCACACATCAACATCTTGATGACAAGT
  GACGACATTGCAACCGGCCTCGGTCAAAACATAAAATTAATCAAATGGATGATTATTATG
  CTCATCAGTATGTTAGCCGGTATTTCGGTAGCCGTAGCTGGATCAATCGTCTTTGTGGGT
  CTTATCGTACCGAATATTAGCAAACGATTATTACCACCAAACTATAAGTATTTAATTCCT
  TTTACTGCATTAGCTGGAGCAATCCTAATGATCATTTCAGACATTGTTGCTCGTATAATA
  ATTAAGCCACTAGAGTTGCCTATCGGTGTCGTTACCGCTGTCATTGGCGCTATTGTCTTA
  ATCTATATTATGAAGAAAGGACGTCAACGCTTATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  996
  

⊟Protein

⊟General

• locus tag: SAUSA300_0116 [new locus tag: SAUSA300_RS00600 ]
• symbol: SirB
• description: iron compound ABC transporter permease SirB
• length: 331
• theoretical pI: 10.5981
• theoretical MW: 35525.2
• GRAVY: 1.22991

⊟Function

• TIGRFAM:
  proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 153.6)
• TheSEED  :
  • Siderophore staphylobactin ABC transporter, permease protein SirB
  Iron acquisition and metabolism Siderophores Siderophore Staphylobactin  Siderophore staphylobactin ABC transporter, permease protein SirB
• PFAM:
  Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 298.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 9
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.24136
  • TAT(Tat/SPI): 0.001711
  • LIPO(Sec/SPII): 0.027406
• predicted transmembrane helices (TMHMM): 9

⊟Accession numbers

• GI: 87160001 NCBI
• RefSeq: YP_492833 NCBI
• UniProt: A0A0H2XE68 UniProt

⊟Protein sequence

• MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFDSENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFVIFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFTAFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILMTSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYKYLIPFTALAGAILMIISDIVARIIIKPLELPIGVVTAVIGAIVLIYIMKKGRQRL

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: sirC < sirB < sirA

⊟Regulation

• regulator: Fur* (repression) regulon

  Fur*

  (TF)

  important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)