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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA0111 [new locus tag: SA_RS00710 ]
- pan locus tag?: SAUPAN000913000
- symbol: sirA
- pan gene symbol?: sirA
- synonym:
- product: lipoprotein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA0111 [new locus tag: SA_RS00710 ]
- symbol: sirA
- product: lipoprotein
- replicon: chromosome
- strand: -
- coordinates: 127549..128541
- length: 993
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1122885 NCBI
- RefSeq: NP_373352 NCBI
- BioCyc: see SA_RS00710
- MicrobesOnline: 102378 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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961ATGAATAAAGTAATTAAAATGCTTGTTGTTACGCTTGCTTTCCTACTTGTTTTAGCAGGA
TGTAGTGGGAATTCAAATAAACAATCATCTGATAACAAAGATAAGGAAACAACTTCAATT
AAACATGCAATGGGTACAACTGAAATTAAAGGGAAACCAAAGCGTGTTGTTACGCTATAT
CAAGGTGCCACTGACGTCGCTGTATCTTTAGGTGTTAAACCTGTAGGTGCTGTAGAATCA
TGGACACAAAAACCGAAATTCGAATACATAAAAAATGATTTAAAAGATACTAAGATTGTA
GGTCAAGAACCTGCACCTAACTTAGAGGAAATCTCTAAATTAAAACCGGACTTAATTGTC
GCGTCAAAAGTTAGAAATGAAAAAGTTTACGATCAATTATCTAAAATCGCACCAACAGTT
TCTACTGATACAGTTTTCAAATTCAAAGATACAACTAAGTTAATGGGGAAAGCTTTAGGG
AAAGAAAAAGAAGCTGAAGATTTACTTAAAAAGTACGATGATAAAGTAGCTGCATTCCAA
AAAGATGCAAAAGCAAAGTATAAAGATGCATGGCCATTGAAAGCTTCAGTTGTTAACTTC
CGTGCTGATCATACAAGAATTTATGCTGGTGGATATGCTGGTGAAATCTTAAATGATTTA
GGATTCAAACGTAATAAAGACTTACAAAAACAAGTTGATAATGGTAAAGATATTATCCAA
CTTACATCTAAAGAAAGCATTCCATTAATGAACGCTGATCATATTTTTGTAGTAAAATCA
GATCCAAATGCGAAAGATGCTGCATTAGTTAAAAAGACTGAAAGCGAATGGACTTCAAGT
AAAGAGTGGAAAAATTTAGACGCAGTTAAAAACAACCAAGTATCTGATGATTTAGATGAA
ATCACTTGGAACTTAGCTGGCGGATATAAATCTTCATTAAAACTTATTGACGATTTATAT
GAAAAGTTAAATATTGAAAAACAATCAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA0111 [new locus tag: SA_RS00710 ]
- symbol: SirA
- description: lipoprotein
- length: 330
- theoretical pI: 9.7617
- theoretical MW: 36743.9
- GRAVY: -0.563636
⊟Function[edit | edit source]
- TIGRFAM: heme ABC transporter, heme-binding protein isdE (TIGR03659; HMM-score: 61.7)and 5 moreputative ABC transporter PGF-CTERM-modified substrate-binding protein (TIGR04281; HMM-score: 30.5)Cellular processes Sporulation and germination sporulation lipoprotein, YhcN/YlaJ family (TIGR02898; HMM-score: 16)proposed F420-0 ABC transporter, periplasmic F420-0 binding protein (TIGR03868; HMM-score: 14.2)Central intermediary metabolism Sulfur metabolism phosphoadenosine phosphosulfate reductase (TIGR02057; EC 1.8.4.8; HMM-score: 14)Protein fate Protein and peptide secretion and trafficking outer membrane assembly lipoprotein YfiO (TIGR03302; HMM-score: 13.2)
- TheSEED :
- Siderophore staphylobactin ABC transporter, substrate-binding protein SirA
- PFAM: Chelatase (CL0043) Peripla_BP_2; Periplasmic binding protein (PF01497; HMM-score: 85.1)and 3 moreno clan defined DUF7537; Family of unknown function (DUF7537) (PF24381; HMM-score: 17.8)DUF6491; Family of unknown function (DUF6491) (PF20101; HMM-score: 12.4)ETRAMP; Malarial early transcribed membrane protein (ETRAMP) (PF09716; HMM-score: 10.5)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 9.68
- Cellwall Score: 0.17
- Extracellular Score: 0.16
- Internal Helix: 1
- DeepLocPro: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 0.9938
- Cell wall & surface Score: 0.0023
- Extracellular Score: 0.0039
- LocateP: Lipid anchored
- Prediction by SwissProt Classification: Extracellular
- Pathway Prediction: Sec-(SPII)
- Intracellular possibility: -0.33
- Signal peptide possibility: 1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: LVLAGCS
- SignalP: Signal peptide LIPO(Sec/SPII) length 20 aa
- SP(Sec/SPI): 0.000361
- TAT(Tat/SPI): 0.000102
- LIPO(Sec/SPII): 0.999306
- Cleavage Site: CS pos: 20-21. LAG-CS. Pr: 0.9999
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: sirC < sirB < sirA
⊟Regulation[edit | edit source]
- regulator: Fur* (repression) regulon
Fur* (TF) important in Iron homeostasis; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
⊟Relevant publications[edit | edit source]
Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)