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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00071
  • pan locus tag?: SAUPAN000911000
  • symbol: SAOUHSC_00071
  • pan gene symbol?: sirC
  • synonym:
  • product: lipoprotein SirC

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00071
  • symbol: SAOUHSC_00071
  • product: lipoprotein SirC
  • replicon: chromosome
  • strand: -
  • coordinates: 76521..77435
  • length: 915
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGATTGGTTCAAGCTTTATACCATTACAACGCGTACTGATGTACTTTATAAATCCAAAT
    GACAGTATGGATCAATTCACTTTAGAAGTATTACGCTTACCTCGCATTACACTTGCGATT
    TTAGCAGGTGCCGCACTAGGAATGAGTGGTTTAATGTTGCAAAATGTATTAAAAAATCCA
    ATTGCCTCACCTGATATTATCGGTATCACAGGTGGTGCTAGCTTAAGTGCTGTTGTCTTT
    ATTGCATTTTTCAGCCATTTAACAATACATTTACTTCCACTATTTGCAGTATTAGGTGGC
    GCAGTTGCAATGATGATACTATTAGTGTTTCAAACGAAAGGACAAATACGCCCGACAACA
    CTCATAATCATCGGTATTTCGATGCAAACGTTGTTTATTGCGCTTGTCCAAGGATTACTC
    ATTACAACGAAGCAATTATCTGCTGCCAAAGCTTATACATGGCTAGTCGGAAGTCTTTAC
    GGTGCTACGTTTAAAGATACAATCATTTTGGGTATGGTTATTTTAGCTGTTGTGCCGTTG
    TTATTTCTTGTTATACCAAAAATGAAAATATCTATACTTGATGACCCTGTAGCGATTGGC
    TTAGGCTTACATGTACAACGTATGAAACTAATCCAATTAATCACTTCTACTATACTCGTA
    TCTATGGCAATCAGTTTAGTAGGTAACATTGGGTTTGTCGGTTTAATCGCACCACATATC
    GCGAAAACAATCGTTCGCGGAAGTTATGCTAAAAAGTTACTAATGTCAGCAATGATTGGT
    GCCATATCAATTGTTATTGCAGACTTAATTGGGCGTACCTTATTCTTGCCTAAAGAAGTG
    CCAGCAGGTGTATTTATTGCTGCTTTTGGTGCCCCATTCTTCATATACTTATTATTAACC
    GTGAAAAAGTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00071
  • symbol: SAOUHSC_00071
  • description: lipoprotein SirC
  • length: 304
  • theoretical pI: 10.7701
  • theoretical MW: 32508.6
  • GRAVY: 1.14539

⊟Function[edit | edit source]

  • TIGRFAM:
    proposed F420-0 ABC transporter, permease protein (TIGR03869; HMM-score: 150.5)
    and 1 more
    Metabolism Transport and binding proteins Unknown substrate anchored repeat-type ABC transporter, permease subunit (TIGR03770; HMM-score: 30.3)
  • TheSEED  :
    • Siderophore staphylobactin ABC transporter, permease protein SirC
    Iron acquisition and metabolism Siderophores Siderophore Staphylobactin  Siderophore staphylobactin ABC transporter, permease protein SirC
  • PFAM:
    Membrane_trans (CL0142) FecCD; FecCD transport family (PF01032; HMM-score: 275.2)
    and 2 more
    ABC-3; ABC 3 transport family (PF00950; HMM-score: 21)
    ISOCOT_Fold (CL0246) Sugar-bind; Putative sugar-binding domain (PF04198; HMM-score: 12.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.009926
    • TAT(Tat/SPI): 0.001683
    • LIPO(Sec/SPII): 0.002536
  • predicted transmembrane helices (TMHMM): 8

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIGSSFIPLQRVLMYFINPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGASLSAVVFIAFFSHLTIHLLPLFAVLGGAVAMMILLVFQTKGQIRPTTLIIIGISMQTLFIALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKISILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGSYAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: Fur* (repression) regulon
    Fur*(TF)important in Iron homeostasis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Suzanne E Dale, M Tom Sebulsky, David E Heinrichs
Involvement of SirABC in iron-siderophore import in Staphylococcus aureus.
J Bacteriol: 2004, 186(24);8356-62
[PubMed:15576785] [WorldCat.org] [DOI] (P p)

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