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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002399
  • pan locus tag?: SAUPAN005966000
  • symbol: bioA
  • pan gene symbol?: bioA
  • synonym:
  • product: adenosylmethionine--8-amino-7-oxononanoate transaminase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002399
  • symbol: bioA
  • product: adenosylmethionine--8-amino-7-oxononanoate transaminase
  • replicon: chromosome
  • strand: -
  • coordinates: 2395815..2397173
  • length: 1359
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATTACACACAACAACTTAAACAAAAAGACTCAGAATATGTTTGGCATCCATTTACA
    CAAATGGGTGTATATAGCAAAGAAGAAGCAATCATCATTGAAAAAGGAAAGGGTAGTTAC
    CTTTACGATACGAATGGCAATAAATATTTAGATGGTTATGCATCGTTGTGGGTCAATGTG
    CATGGTCATAATAACAAATACTTAAATAAGGTAATTAAAAAGCAACTCAATAAAATTGCC
    CATTCTACGCTGCTAGGATCATCAAATATTCTGTCAATAGAACTTGCGGAAAAATTAATC
    GAAATCACGCCAAGTAATCTAAGAAAAGTATTTTATTCTGATACAGGCAGTGCGTCTGTT
    GAAATCGCAATAAAGATGGCATATCAGTATTGGAAAAATATTGATAGAGAAAAATATGCC
    AAGAAAAACAAGTTTATAACGCTAAATCACGGCTATCATGGGGATACGATTGGTGCGGTA
    AGCGTTGGTGGTATCAAGACCTTTCATAAAATATTTAAAGACTTAATATTTGAGAATATT
    CAAGTAGAAAGCCCATCTTTTTATCGCAGTAATTACGATACTGAAAATGAAATGATGACA
    GCTATTTTAACGAATATAGAGCAAATTTTAATTGAAAGAAATGATGAAATCGCAGGGTTT
    ATATTGGAACCGTTGATTCAAGGTGCGACAGGCTTGTTTGTTCATCCTAAAGGCTTTTTG
    AAAGAAGTCGAGAAATTGTGCAAAAAATACGATGTCTTATTAATTTGTGATGAGGTAGCA
    GTTGGTTTTGGGAGAACTGGAAAGATGTTTGCATGCAATCATGAAGATGTTCAACCGGAT
    ATTATGTGTTTAGGTAAGGCGATTACTGGTGGCTATTTACCACTTGCAGCTACATTGACA
    TCTCAAAAAATATACAATGCATTTTTAAGTGATTCGCATGGTGTCAATACCTTTTTCCAT
    GGTCATACATACACCGGAAATCAAATCGTTTGTACGGTTGCATTAGAAAATATAAGACTT
    TATGAAAAACGTAAGTTATTGTCACATATTGAAACGACATCATCAACACTTGAGAAACAG
    TTACATGCGCTGAAGCGTCATCGAAATGTTGGTGATGTAAGAGGACGAGGCTTAATGTTT
    GGAGTTGAATTAGTTACAGATAAAGATAGTAAAACGCCGTTAGAAATTGAAAAAGTTGAA
    CGTATTGTACGTAAATGTAAAGAAAACGGGCTAATGATTAGAAATTTAGAAAATGTCATT
    ACGTTTGTGCCAGTGTTAAGTATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTT
    AAAAAGGCAGTACATAACATTTTAGATAGGAAGTGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002399
  • symbol: BioA
  • description: adenosylmethionine--8-amino-7-oxononanoate transaminase
  • length: 452
  • theoretical pI: 9.20767
  • theoretical MW: 51434.1
  • GRAVY: -0.275442

⊟Function[edit | edit source]

  • reaction:
    EC 2.6.1.62?  ExPASy
    Adenosylmethionine--8-amino-7-oxononanoate transaminase S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 548.8)
    and 11 more
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 268)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 228.5)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 202.2)
    Metabolism Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 198.3)
    Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 193.8)
    Metabolism Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 191.1)
    Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 168.1)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 145.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 128.7)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 99.2)
    Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 12.4)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 363.1)
    and 1 more
    no clan defined Pox_L5; Poxvirus L5 protein family (PF04872; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.7508
    • Cytoplasmic Membrane Score: 0.0686
    • Cell wall & surface Score: 0.0025
    • Extracellular Score: 0.1782
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.046625
    • TAT(Tat/SPI): 0.000523
    • LIPO(Sec/SPII): 0.00274
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNILSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRKCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA* (repression) regulon
    BirA*(TF)important in Biotin biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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