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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2372 [new locus tag: SAUSA300_RS13105 ]
  • pan locus tag?: SAUPAN005966000
  • symbol: bioA
  • pan gene symbol?: bioA
  • synonym:
  • product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_2372 [new locus tag: SAUSA300_RS13105 ]
  • symbol: bioA
  • product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2548884..2550242
  • length: 1359
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATTACACACAACAACTTAAACAAAAAGACTCAGAATATGTTTGGCATCCATTTACA
    CAAATGGGTGTATATAGCAAAGAAGAAGCAATCATCATTGAAAAAGGAAAGGGTAGTTAC
    CTTTACGATACGAATGGCAATAAATATTTAGATGGTTATGCATCGTTGTGGGTCAATGTG
    CATGGTCATAATAACAAATACTTAAATAAGGTAATTAAAAAGCAACTCAATAAAATTGCC
    CATTCTACGCTGCTAGGATCATCAAATATTCCGTCAATAGAACTTGCGGAAAAATTAATC
    GAAATCACGCCAAGTAATCTAAGAAAAGTATTTTATTCTGATACAGGCAGTGCGTCTGTT
    GAAATCGCAATAAAGATGGCATATCAGTATTGGAAAAATATTGATAGAGAAAAATATGCC
    AAGAAAAACAAGTTTATAACGCTAAATCACGGTTATCATGGGGATACGATTGGTGCGGTA
    AGTGTTGGTGGTATCAAGACCTTTCATAAAATATTTAAAGACTTAATATTTGAGAATATT
    CAAGTAGAAAGCCCATCTTTCTATCGCAGTAATTACGATACTGAAAATGAAATGATGACA
    GCTATTTTAACGAATATAGAGCAAATTCTAATTGAAAGAAATGATGAAATCGCAGGGTTT
    ATATTGGAACCGTTGATTCAAGGTGCGACAGGCTTGTTTGTTCATCCTAAAGGCTTTTTG
    AAAGAAGTCGAGAAATTGTGCAAAAAATACGATGTCTTATTAATTTGTGATGAGGTAGCA
    GTTGGTTTTGGGAGAACTGGAAAGATGTTTGCATGCAATCATGAAGATGTTCAACCGGAT
    ATTATGTGTTTAGGTAAGGCGATTACTGGTGGCTACTTACCACTTGCAGCTACATTGACA
    TCTAAAAAAATATACAATGCATTTTTAAGTGATTCGCATGGTGTGAATACCTTTTTCCAT
    GGTCATACATACACCGGAAATCAAATCGTTTGTACGGTTGCATTAGAAAATATAAGACTT
    TATGAAAAACGTAAGTTATTGTCACATATTGAAACGACATCATCAACACTTGAGAAACAG
    TTACATGCGCTGAAGCGTCATCGAAATGTTGGTGATGTAAGAGGACGAGGCTTAATGTTT
    GGAGTTGAATTAGTTACAGATAAAGATAGTAAAACGCCGTTAGAAATTGAAAAAGTTGAA
    CGTATTGTACGTAATTGTAAAGAAAACGGGCTAATGATTAGAAATTTAGAAAATGTCATT
    ACGTTTGTGCCAGTGTTAAGTATGTCAAATAAAGAAGTGAAAACGATGGTACGTATTTTT
    AAAAAGGCAGTACATAACATTTTAGATAGGAAGTGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1359

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_2372 [new locus tag: SAUSA300_RS13105 ]
  • symbol: BioA
  • description: adenosylmethionine-8-amino-7-oxononanoate aminotransferase
  • length: 452
  • theoretical pI: 9.20767
  • theoretical MW: 51404
  • GRAVY: -0.287389

⊟Function[edit | edit source]

  • reaction:
    EC 2.6.1.62?  ExPASy
    Adenosylmethionine--8-amino-7-oxononanoate transaminase S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin adenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 550.6)
    and 11 more
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 269.6)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 226.6)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 202.2)
    Metabolism Energy metabolism Amino acids and amines succinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 201.7)
    Metabolism Central intermediary metabolism Other 2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 192.1)
    Metabolism Central intermediary metabolism Polyamine biosynthesis putrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 189.2)
    Cellular processes Cellular processes Adaptations to atypical conditions diaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 168.4)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 144.1)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin glutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 128.6)
    Metabolism Central intermediary metabolism Other 4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 99)
    Metabolism Energy metabolism Amino acids and amines glycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 12.5)
  • TheSEED  :
    • Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Biotin Biotin biosynthesis  Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 386.2)
    and 1 more
    no clan defined Pox_L5; Poxvirus L5 protein family (PF04872; HMM-score: 12.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 0
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.046625
    • TAT(Tat/SPI): 0.000523
    • LIPO(Sec/SPII): 0.00274
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDTNGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEITPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNHGYHGDTIGAVSVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIAGFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHEDVQPDIMCLGKAITGGYLPLAATLTSKKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVALENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKTPLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDRKC

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA (repression) regulon
    BirA(TF)important in Biotin biosynthesis; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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