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FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002398
  • pan locus tag?: SAUPAN005965000
  • symbol: bioB
  • pan gene symbol?: bioB
  • synonym:
  • product: biotin synthase BioB

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002398
  • symbol: bioB
  • product: biotin synthase BioB
  • replicon: chromosome
  • strand: -
  • coordinates: 2394806..2395813
  • length: 1008
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAATTTGGCTAAACGCATATTACAAGGGGAACAATTAACAAAAGAGACTGTATTGAAA
    ATTTATGAGGATACTAATATTGATACCTTAGATTTATTAAATGAGGCGTACATTTTAAGA
    AAACATTATTTTGGTAAAAAAGTAAAATTAAACATGATTTTAAATGCTAAAAGTGGTATA
    TGTCCTGAAAATTGTGGGTACTGTGGACAATCACGAGATATTAAACAAAAACAGCGATAT
    GCTTTAATTCCAGAGGAAAAAATTATCGATGGAGCAAAGGTGGCACATGATAATCATATT
    GGAACATATTGTATTGTTATGAGTGGTAGAGGACCGAGCGATAAAGAAGTTGATCATATT
    AGTAATACTGTAAGAACGATTAAATCTCAACACCCGCAACTAAAAATCTGTGCATGTTTA
    GGATTAACGAATGACGAACAAGCTAAGAAACTTAAGTCAGCTGGTGTAGACAGATATAAC
    CACAATATTAATACAAGTGAAAATTACCATGATAACGTCGTGACAACGCATAGTTATAAA
    GATAGAACAGATACGATAGAACTAATGAAAGCGAATAATATATCACCATGTTCTGGCGTG
    ATTTGTGGTATGGGAGAATCTAATCAAGATATTGTTGATATGGCATTTGCTTTAAAAGAA
    ATGGATGCCGACAGTATTCCGATTAATTTTTTGCATCCAATCAAAGGCACAAAGTTTGGA
    AGCATGGATGATTTAACACCAATGAAATGTTTAAGAATCGTAGCATTATTCCGATTAATC
    AATCCTACGAAAGAAATTCGTATTGCTGGAGGAAGAGAGGTCAACTTACGTTCGTTACAG
    CCATTAGCATTAAAAGCGGCGAATTCAATATTTGTCGGTGATTATCTAATTACTGGTGGG
    CAACCGAACCAATTAGACTACGATATGATTAATGATTTGGGCTTTGAAATTGATTATGAC
    ACTTGTGAAAATAAGGAAAACAAGAATGATGTTTCAAGAGCAAATTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1008

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002398
  • symbol: BioB
  • description: biotin synthase BioB
  • length: 335
  • theoretical pI: 6.97037
  • theoretical MW: 37544.7
  • GRAVY: -0.445373

⊟Function[edit | edit source]

  • reaction:
    EC 2.8.1.6?  ExPASy
    Biotin synthase Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Biotin biotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 377.7)
    and 17 more
    Genetic information processing Protein fate Protein modification and repair [FeFe] hydrogenase H-cluster radical SAM maturase HydE (TIGR03956; HMM-score: 84.7)
    Metabolism Amino acid biosynthesis Other pyrrolysine biosynthesis radical SAM protein (TIGR03910; HMM-score: 80.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiazole biosynthesis protein ThiH (TIGR02351; HMM-score: 54.5)
    Genetic information processing Protein fate Protein modification and repair 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50)
    Metabolism Energy metabolism Methanogenesis 5,10-methenyltetrahydromethanopterin hydrogenase cofactor biosynthesis protein HmdB (TIGR03957; HMM-score: 50)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Lipoate lipoyl synthase (TIGR00510; EC 2.8.1.8; HMM-score: 44.2)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone putative menaquinone biosynthesis radical SAM enzyme, SCO4494 family (TIGR03700; HMM-score: 40.7)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone dehypoxanthine futalosine cyclase (TIGR03699; HMM-score: 34.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 30.6)
    Hypothetical proteins Conserved radical SAM domain protein, CofH subfamily (TIGR00423; HMM-score: 28.7)
    Unknown function Enzymes of unknown specificity radical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 23.4)
    radical SAM/SPASM domain protein, ACGX system (TIGR04340; HMM-score: 19.8)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Molybdopterin probable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 18.6)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Other 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofG subunit (TIGR03550; EC 2.5.1.77; HMM-score: 18.4)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal kinase (TIGR00687; EC 2.7.1.35; HMM-score: 15.8)
    magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase (TIGR02026; HMM-score: 15.1)
    His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 12.6)
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    no clan defined BATS; Biotin and Thiamin Synthesis associated domain (PF06968; HMM-score: 91.2)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 82)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.8026
    • Cytoplasmic Membrane Score: 0.1284
    • Cell wall & surface Score: 0.001
    • Extracellular Score: 0.068
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.025518
    • TAT(Tat/SPI): 0.002236
    • LIPO(Sec/SPII): 0.001056
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYFGKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEKIIDGAKVAHDNHIGTYCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYNHNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFALKEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNLRSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRAN

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: BirA* (repression) regulon
    BirA*(TF)important in Biotin biosynthesis;  regulation predicted or transferred from N315 and NCTC 8325  [2]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Blanca Taboada, Karel Estrada, Ricardo Ciria, Enrique Merino
    Operon-mapper: a web server for precise operon identification in bacterial and archaeal genomes.
    Bioinformatics: 2018, 34(23);4118-4120
    [PubMed:29931111] [WorldCat.org] [DOI] (I p)
  2. ↑ Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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